80 research outputs found
SMSLib - biblioteca C++ do Sting Millennium Suite.
Organização lógica do SMS. Descrição da SMSLib. Leitura de arquivos PDB. Leitura de arquivos HSSP. Leitura de arquivos com parùmetros simples. Cålculo e leitura de contatos. Cålculo e leitura de Dihedral Angels.bitstream/CNPTIA/9898/1/comuntec39.pdfAcesso em: 30 maio 2008
Sequence diversity within the HA-1 gene as detected by melting temperature assay without oligonucleotide probes
BACKGROUND: The minor histocompatibility antigens (mHags) are self-peptides derived from common cellular proteins and presented by MHC class I and II molecules. Disparities in mHags are a potential risk for the development of graft-versus-host disease (GvHD) in the recipients of bone marrow from HLA-identical donors. Two alleles have been identified in the mHag HA-1. The correlation between mismatches of the mHag HA-1 and GvHD has been suggested and methods to facilitate large-scale testing were afterwards developed. METHODS: We used sequence specific primer (SSP) PCR and direct sequencing to detect HA-1 gene polymorphisms in a sample of 131 unrelated Italian subjects. We then set up a novel melting temperature (Tm) assay that may help identification of HA-1 alleles without oligonucleotide probes. RESULTS: We report the frequencies of HA-1 alleles in the Italian population and the presence of an intronic 5 base-pair deletion associated with the immunogeneic allele HA-1(H). We also detected novel variable sites with respect to the consensus sequence of HA-1 locus. Even though recombination/gene conversion events are documented, there is considerable linkage disequilibrium in the data. The gametic associations between HA-1(R/H )alleles and the intronic 5-bp ins/del polymorphism prompted us to try the Tm analysis with SYBR(Âź )Green I. We show that the addition of dimethylsulfoxide (DMSO) during the assay yields distinct patterns when amplicons from HA-1(H )homozygotes, HA-1(R )homozygotes, and heterozygotes are analysed. CONCLUSION: The possibility to use SYBR(Âź )Green I to detect Tm differences between allelic variants is attractive but requires great caution. We succeeded in allele discrimination of the HA-1 locus using a relatively short (101 bp) amplicon, only in the presence of DMSO. We believe that, at least in certain assets, Tm assays may benefit by the addition of DMSO or other agents affecting DNA strand conformation and stability
STING Millennium: a web-based suite of programs for comprehensive and simultaneous analysis of protein structure and sequence.
Sting Millennium suite intrinsics. SMS organization. Sting Millennium Modes. Sting Millennium Modules. Millennium Features. Example of Sting Millennium Application.Na publicação: Paula R. Kuser
HLA-class I markers and multiple sclerosis susceptibility in the Italian population
Previous studies reported an association with multiple sclerosis (MS) of distinct HLA-class I markers, namely HLA-A*02, HLA-Cw*05 and MOG-142L. In this work, we tested the association with MS of A*02 and Cw*05 in 1273 Italian MS patients and 1075 matched controls, which were previously analyzed for MOG-142, and explored the relationship among these three markers in modulating MS risk. HLA-A*02 conferred a statistically robust MS protection (odds ratio, OR=0.61; 95% confidence intervals, CI=0.51â0.72, P<10â9), which was independent of DRB1*15 and of any other DRB1* allele and remained similar after accounting for the other two analyzed class I markers. Conversely, the protective effect we previously observed for MOG-142L was secondary to its linkage disequilibrium with A*02. Cw*05 was not associated considering the whole sample, but its presence significantly enhanced the protection in the HLA-A*02-positive group, independently of DRB1: the OR conferred by A*02 in Cw*05-positive individuals (0.22, 95% CI=0.13â0.38) was significantly lower than in Cw*05-negative individuals (0.69, 95% CI=0.58â0.83) with a significant (P=4.94 Ă 10â5) multiplicative interaction between the two markers. In the absence of A*02, Cw*05 behaved as a risk factor, particularly in combination with DRB1*03 (OR=3.89, P=0.0006), indicating that Cw*05 might be a marker of protective or risk haplotypes, respectively
Inibidor da ação do etileno na conservação pós-colheita de Chrysanthemum morifolium Ramat cv. Dragon
Qualidade de maçãs 'Royal gala' submetidas ao dano mecùnico por impacto e aplicação de 1-metilciclopropeno em dois sistemas comerciais de armazenamento
Efeito do ĂĄcido indolbutĂrico no enraizamento de estacas de ramos semilenhosos de pessegueiro
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