3,105 research outputs found

    De novo Development and Characterization of Tetranucleotide Microsatellite Loci Markers from a Southeastern Population of the House Finch (Haemorhous mexicanus)

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    Microsatellites are short tandem repeats (e.g. TAGATAGA) of base pairs in a species’ genome. High mutation rates in these regions produce variation in the number of repeats across individuals that can be utilized to study patterns of population- and landscape-level genetics and to determine parentage genetically. In this project our objective was to develop microsatellite markers for the House Finch, Haemorhous mexicanus. This species has become one of the most well-studied species of songbirds due to its unique geographical, evolutionary, and epidemiological history. Using mist-nets we captured birds on the Arkansas Tech University campus and collected blood samples to obtain genomic DNA. Samples were processed in The Field Museum’s Pritzker Laboratory for Molecular Systematics and Evolution, where we fragmented genomic DNA and isolated fragments that contained potential microsatellites using specially designed biotin labelled probes. These DNA fragments were transformed into competent E. coli cells which were then PCR-amplified and Sanger sequenced. After sequencing DNA fragments from approximately 500 E. coli colonies, we designed and characterized a set of 13 tetranucleotide microsatellite loci. The average number of alleles and heterozygosity found in 12 individuals from Arkansas was 8.69 and 0.80, respectively. This finalized set of microsatellites can be utilized by researchers to determine parentage and characterize genetic differences across House Finch populations

    A Laboratory Infection of Alfalfa Weevil, \u3ci\u3eHypera Postica\u3c/i\u3e (Coleoptera: Curculionidae), Larvae With the Fungal Pathogen \u3ci\u3eZoophthora Phytonomi\u3c/i\u3e (Zygomycetes: Entomophthoraceae)

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    Larvae of the alfalfa weevil, Hypera postica, were infected by an in vitro colony of Zoophthora phytonomi. Two spore types (infective conidia, and resting spores) were produced from infection trials. The spore type produced may be influenced by the physiological state of the larvae. Trials using field collected larvae which would produce diapausing adults formed both conidia and resting spores. Trials using larvae from a nondiapausing colony, however, formed only resting spores

    The local space density of dwarf galaxies

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    We estimate the luminosity function of field galaxies over a range of ten magnitudes (-22 < M_{B_J} < -12 for H_0 = 100 km/s/Mpc) by counting the number of faint APM galaxies around Stromlo-APM redshift survey galaxies at known distance. The faint end of the luminosity function rises steeply at M_{B_J} \approx -15, implying that the space density of dwarf galaxies is at least two times larger than predicted by a Schechter function with flat faint-end slope. Such a high abundance of dwarf galaxies at low redshift can help explain the observed number counts and redshift distributions of faint galaxies without invoking exotic models for galaxy evolution.Comment: 20 pages, 5 included postscript figures, uses AAS LaTex macros. Accepted for publication in the Astrophysical Journal. Two figures and associated discussion added; results and conclusions unchange

    Disease as a Larval Mortality Factor in Alfalfa Weevil, \u3ci\u3eHypera Postica\u3c/i\u3e (Coleoptera: Curculionidae) Populations in Illinois

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    During the 1974 growing season, larvae of the alfalfa weevil, Hypera postica (Gyllenhal), were examined for pathogens. Three larvae out of 715 examined were infected with a microsporidium. This infection was present in both Washington and Mason counties in Illinois
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