Isotropic magnetometry with simultaneous excitation of orientation and alignment CPT resonances

Atomic magnetometers have very high absolute precision and sensitivity to magnetic fields but suffer from a fundamental problem: the vectorial or tensorial interaction of light with atoms leads to "dead zones", certain orientations of magnetic field where the magnetometer loses its sensitivity. We demonstrate a simple polarization modulation scheme that simultaneously creates coherent population trapping (CPT) in orientation and alignment, thereby eliminating dead zones. Using $^{87}$Rb in a 10 Torr buffer gas cell we measure narrow, high-contrast CPT transparency peaks in all orientations and also show absence of systematic effects associated with non-linear Zeeman splitting.Comment: 4 pages, 4 figure

Glutamate Uptake into Synaptic Vesicles: Competitive Inhibition by Bromocriptine

The ATP-dependent uptake of l-glutamate into synaptic vesicles has been well characterized, implicating a key role for synaptic vesicles in glutamatergic neurotransmission. In the present study, we provide evidence that vesicular glutamate uptake is selectively inhibited by the pep-tide-containing halogenated ergot bromocriptine. It is the most potent inhibitor of the agents tested; the IC 5 o was de-termined to be 22 Μ M . The uptake was also inhibited by other ergopeptines such as ergotamine and ergocristine, but with less potency. Ergots devoid of the peptide moiety, however, such as ergonovine, lergotrile, and methysergide, had little or no effect. Although bromocriptine is known to elicit dopaminergic and serotonergic effects, its inhibitory effect on vesicular glutamate uptake was not mimicked by agents known to interact with dopamine and serotonin receptors. Kinetic data suggest that bromocriptine competes with glutamate for the glutamate binding site on the glutamate trans-locator. It is proposed that this inhibitor could be useful as a prototype probe in identifying and characterizing the vesicular glutamate translocator, as well as in developing a more specific inhibitor of the transport system.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/66274/1/j.1471-4159.1989.tb09258.x.pd

Theory of Dicke narrowing in coherent population trapping

The Doppler effect is one of the dominant broadening mechanisms in thermal vapor spectroscopy. For two-photon transitions one would naively expect the Doppler effect to cause a residual broadening, proportional to the wave-vector difference. In coherent population trapping (CPT), which is a narrow-band phenomenon, such broadening was not observed experimentally. This has been commonly attributed to frequent velocity-changing collisions, known to narrow Doppler-broadened one-photon absorption lines (Dicke narrowing). Here we show theoretically that such a narrowing mechanism indeed exists for CPT resonances. The narrowing factor is the ratio between the atom's mean free path and the wavelength associated with the wave-vector difference of the two radiation fields. A possible experiment to verify the theory is suggested.Comment: 6 pages, 2 figures; Introduction revise

Synaptic Vesicular Glutamate Uptake: Modulation by a Synaptosomal Cytosolic Factor

We have demonstrated previously that L-glutamate is taken up into isolated synaptic vesicles in an ATP-dependent manner, supporting the neurotransmitter role of this acidic amino acid. We now report that a nerve terminal cytosolic factor inhibits the ATP-dependent vesicular uptake of glutamate in a dose-dependent manner. This factor appears to be a protein with a molecular weight >100,000, as estimated by size exclusion chromatography, and is precipitated by ammonium sulfate (40% saturation). The inhibitory factor is inactivated by heating to 100°C. Proteolytic digestion of the ammonium sulfate fraction by trypsin or chymotrypsin did not reduce, but rather increased slightly, the inhibition of glutamate uptake. Unlike the native factor, the digest retained inhibitory activity after heating, suggesting that proteolytic digestion may generate active fragments. The inhibition of ATP-dependent vesicular glutamate uptake is not species-specific, as the factor obtained from both rat and bovine brains produced an equal degree of inhibition of glutamate uptake into vesicles of each species. These observations raise the possibility that vesicular uptake of glutamate may be regulated by an endogenous factor in vivo.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/66195/1/j.1471-4159.1990.tb01212.x.pd

Automated Scalable Heat Shock Modification for Standard Aquatic Housing Systems

Heat shock is a common technique for inducible gene expression system in a variety of organisms. Heat shock treatment of adult zebrafish is more involved and generally consists of manually transferring fish between housing rack tanks and preheated water tanks or the use of timed heaters in stand-alone aquaria. To avoid excessive fish handling and to take advantage of the continuous flow of a standard housing rack, proposed modifications consisted of installing an aquarium heater inside each tank, manually setting the heater to reach heat shocking temperatures (>37°C) and, after that, testing that every tank responded equally. To address the limitations in the existing systems, we developed a novel modification of standard zebrafish housing racks to perform heat shock treatment in conditions of continuous water flow. By adding an extra manifold to the housing rack and connecting it to a recirculating bath to create a parallel water flow system, we can increase the temperature from standard conditions (28.5°C) to heat shock conditions with high precision (38.0?38.3°C, mean±SD=38.1°C±0.14°C) and minimal variation among experimental tanks (coefficient of variation [CV]=0.04%). This means that there is virtually no need for laborious pretreatment calibrations or continuous adjustments to minimize intertank variation. To test the effectiveness of our design, we utilized this system to induce enhanced green fluorescent protein (EGFP) expression in hsp70-EGFP fish and performed a fin regeneration experiment with hsp70l:dnfgfr1-EGFP fish to confirm that heat-induced gene expression reached physiological levels. In summary, our newly described aquatic heat shock system minimizes effort during heat shock experiments, while ensuring the best water quality and fish welfare and facilitating large heat shock settings or the use of multiple transgenic lines for both research and teaching experiments.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/140304/1/zeb.2015.1087.pd

ATP-Dependent Glutamate Uptake into Synaptic Vesicles from Cerebellar Mutant Mice

The ATP-dependent glutamate uptake system in synaptic vesicles prepared from mouse cerebellum was characterized, and the levels of glutamate uptake were investigated in the cerebellar mutant mice, staggerer and weaver, whose main defect is the loss of cerebellar granule cells, and the nervous mutant, whose main defect is the loss of Purkinje cells. The ATP-dependent glutamate uptake is stimulated by low concentrations of chloride, is insensitive to aspartate, and is inhibited by agents known to dissipate the electrochemical proton gradient. These properties are similar to those of the glutamate uptake system observed in the highly purified synaptic vesicles prepared from bovine cortex. The ATP-dependent glutamate uptake system is reduced by 68% in the staggerer and 57–67% in the weaver mutant; these reductions parallel the substantial loss of granule cells in those mutants. In contrast, the cerebellar levels of glutamate uptake are not altered significantly in the nervous mutant, which has lost Purkinje cells, but not granule cells. In view of evidence that granule cells are glutamatergic neurons and Purkinje cells are GABAergic neurons, these observations support the notion that the ATP-dependent glutamate uptake system is present in synaptic vesicles of glutamatergic neurons.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/66449/1/j.1471-4159.1988.tb03068.x.pd

Ramsey-like measurement of the decoherence rate between Zeeman sub-levels

Two-photon processes that involve different sub-levels of the ground state of an atom, are highly sensitive to depopulation and decoherence within the ground state. For example, the spectral width of electromagnetically induced transparency resonances in $\Lambda-$type system, are strongly affected by the ground state depopulation and decoherence rates. We present a direct measurement of decay rates between hyperfine and Zeeman sub-levels in the ground state of $^{87}$Rb vapor. Similar to the relaxation-in-the-dark technique, pumping lasers are used to pre-align the atomic vapor in a well defined quantum state. The free propagation of the atomic state is monitored using a Ramsey-like method. Coherence times in the range 1-10 ms were measured for room temperature atomic vapor. In the range of the experimental parameters used in this study, the dominant process inducing Zeeman decoherence is the spin-exchange collisions between rubidium atoms.Comment: 7 pages, 7 figure

Phosphoglycerates and Protein Phosphorylation: Identification of a Protein Substrate as Glucose-1,6-Bisphosphate Synthetase

We have previously reported the occurrence of two endogenous protein phosphorylation systems in mammalian brain that are enhanced in the presence of 3-phosphoglycerate (3PG) and ATP. We present here a study of one of these systems, the phosphorylation of the 72-kDa protein (3PG-PP 72 ). This system was separated into the substrate, 3PG-PP 72 , and a kinase by ammonium sulfate fractionation, hydroxyapatite chromatography, and hydrophobic interaction HPLC. The substrate protein was shown to be directly phosphorylated with [1- 32 P]1,3-bisphosphoglycerate ([1- 32 P]1,3BPG) with an apparent K m of 1.1 n M . Nonradioactive 1,3BPG inhibited 32 P incorporation in the presence of [Γ- 32 P]ATP and 3PG. Phosphopeptide mapping and phosphoamino acid analyses indicated that the site of phosphorylation of 3PG-PP 72 observed in the presence of 3PG and ATP is a serine residue identical to that observed with [1- 32 P]1,3BPG. Moreover, [ 32 P]phosphate incorporated into 3PG-PP 72 in the presence of 3PG and ATP was removed by subsequent incubation with glucose-1-phosphate or glucose-6-phosphate. Finally, 3PG-PP 72 showed chromatographic behaviors identical to those of glucose-1,6-bisphosphate (G1,6P 2 ) synthetase. Based upon these observations, we conclude that 3PG-PP 72 is G1,6P 2 synthetase and that it is phosphorylated directly by 1,3BPG, which is formed from 3PG and ATP by 3PG kinase present in a crude 3PG-PP 72 preparation.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/66052/1/j.1471-4159.1991.tb02028.x.pd