Mutational analysis of the potential catalytic residues of the VV G1L metalloproteinase

The vaccinia virus G1L open-reading frame is predicted to be a metalloproteinase based upon the presence of a conserved zinc-binding motif. Western blot analysis demonstrates G1L undergoes proteolytic processing during the course of infection, although the significance of this event is unknown. In order to determine which amino acid residues are important for G1L activity, a plasmid-borne library of G1L constructs containing mutations in and about the active site was created. Transient expression analysis coupled with a trans complementation assay of a conditionally-lethal mutant virus suggest that, of the mutants, only glutamic acid 120 is non-essential for G1L processing to occur

Oral misoprostol solution in comparison to vaginal misoprostol for induction of labour in a randomized controlled trial

Background: With more than 15% of all gravid women requiring prostaglandins in cervical ripening and labour induction. However, evidence is not clear about the preferred route or dose of the drug. So, this study was designed with objectives to compare the induction delivery interval and safety of titrated oral misoprostol solution with vaginal misoprostol for labour induction in term primigravida women.Methods: This randomized controlled clinical trial was conducted on a total of 100 patients randomly selected among primigravida at term women undergoing induction of labour for obstetric or medical indication for labour induction in Ain Shams University Maternity Hospital. They were divided into two Groups: Group I: patients undergoing induction of labour using misoprostol oral solution and Group II: patients undergoing induction of labour using vaginal misoprostol.Results: Oral misoprostol solution has less induction delivery duration and less side effects than vaginal misoprostol. The induction-delivery time with the oral route compared to the vaginal one (15.2 versus 20.3 hours respectively) with significant p-value (<0.001).Conclusions: Titrated oral misoprostol is safe and effective for labour induction in primigravida patients with unfavorable cervix

The Relationship of Metabolic Syndrome and Aerobic Exercise in Adult Male Fire Fighters

Previous studies have concluded that incidence of Metabolic Syndrome may be greater in male firefighters than in the US male population. A recent study reported that approximately 31% of firefighters were positive for Metabolic Syndrome; compared to 24% for the U.S. male population (NCEPIII). Participation in aerobic exercise may decrease the risk of Metabolic Syndrome as cardiac risk factors are improved. This study aims to determine the effect of aerobic exercise participation on the prevalence of metabolic syndrome in a sample of male firefighters. Purpose: To examine the effect of aerobic exercise participation on the prevalence of Metabolic Syndrome in a sample of adult male firefighters. Methods: As a part of an annual physical exam, 177 male firefighters (average age=36) participated in an evaluation of risk factors associated with Metabolic Syndrome as defined by NCEP III. Metabolic Syndrome is defined as having the presence of three or more of the following: Waist circumference \u3e 40”, HDL Cholesterol \u3c 40 mg/dl, Triglycerides \u3e 150 mg/dl, Blood Glucose \u3e 110 mg/dl and resting blood pressure \u3e 130/85 mm Hg. Aerobic exercise participation was determined by self reported activity from a standard health history questionnaire. Subjects were classified for aerobic exercise participation as: aerobic exercise participation of less than or equal to 1 day per week (LOW), 2 to 3 days per week (MOD), and aerobic exercise participation greater than or equal to 4 days per week (HIGH). Results: The subjects were divided into groups based on the level of weekly aerobic activity. All data were analyzed using a Chi Square test (p \u3c 0.05). Of the 177 firefighters tested 46.3% (82) reported LOW, 48% (85) reported MOD, and 5.6% (10) reported HIGH weekly aerobic activity. The prevalence of Metabolic Syndrome for LOW was 25.6% (21) while the prevalence of Metabolic Syndrome for MOD was 15.3% (13). No one in the HIGH group met the criteria for Metabolic Syndrome. Conclusion: Participation in aerobic exercise appears to affect the incidence of metabolic syndrome in adult male fire fighters

EFFECT OF DIFFERENT ALKALI CONCENTRATIONS DURING THE REFINING PROCESS ON THE FATTY ACID COMPOSITION OF SUNFLOWER AND SOYBEAN OILS

The effect of NaOH and KOH at concentrations of 12, 14 and 16 Bé° during the refining of sunflower and soybean oils on the fatty acid composition was studied using GLC technique. The quantity of linolenic, linoleic, oleic, palmitic and stearic acid as well as the USFA (unsaturated fatty acids): SFA (saturated fatty acids) ratio were measured. Only slight changes in the quantity of individual fatty acids were found particularly at higher alkali concentrations. The changes in the USFA : SFA ratio compared with crude or semirefined oil were not significant

Transmission Studies of Left-handed Materials

Left-handed materials are studied numerically using an improved version of the transfer-matrix method. The transmission, reflection, the phase of the reflection and the absorption are calculated and compared with experiments for both single split-ring resonators (SRR) with negative permeability and left-handed materials (LHMs) which have both the permittivity and permeability negative. Our results suggest ways of positively identifying materials that have both permittivity and permeability negative, from materials that have either permeability or permittivity negative

A review of size and geometrical factors influencing resonant frequencies in metamaterials

Although metamaterials and so-called left-handed media have originated from theoretical considerations, it is only by their practical fabrication and the measurement of their properties that they have gained credibility and can fulfil the potential of their predicted properties. In this review we consider some of the more generally applicable fabrication methods and changes in geometry as they have progressed, exhibiting resonant frequencies ranging from radio waves to the visible optical region