Those Doggone Police: Insufficient Training, Canine Companion Seizures, and Colorado’s Solution

This Comment advocates that California adopt Colorado’s Dog Protection Act or a similar measure mandating police training for dog interactions and implementing specific procedures by law enforcement agencies to reduce dog shootings by police. With the increasing occurrence and coverage of police dog shootings, the need for more adequate law enforcement training on animal encounters is ever present. Additional training, as recently mandated by the Colorado legislature, would minimize police dog shootings. It would also help clarify the applicability of 42 U.S.C. section 1983, the federal statute permitting recovery for the loss of a dog due to a police shooting. Part II focuses on recent developments in animal law illustrating society’s highly empathetic view of dogs. It also outlines the legal classification of dogs as personal property and the constitutional amendment implications when police shoot a dog, highlighting the disparity between the treatment of canine companions and police K9s. Part III discusses 42 U.S.C. section 1983 as an avenue for recovery after a law enforcement officer seizes a canine companion, providing a thorough breakdown of the elements and defenses applicable to an owner’s claim and analyzing Rosby’s potential for success in such a claim. Part IV provides a detailed overview of current developments, summarizes the U.S. Department of Justice’s publication regarding animal encounters, explains Colorado’s Dog Protection Act while evaluating its overall purpose and goals as well as its implications for section 1983 liability, and reviews existing California animal welfare laws. Part V advocates for California to adopt a measure increasing police training and protecting canine companions

Effects of Formulated Feed on water Quality in Fingerling Waleye Production Ponds

Six 0.04-ha plastic-lined ponds were used at the Iowa Department of Natural Resources’ Rathbun Fish Culture and Research Facility to evaluate the use of supplemental fish food on walleye (Sander vitreus) fingerling growth and survival, and on the benthic invertebrate community. Walleye were stocked 3-4 days post hatch on 2 May 2003, and harvested 5-6 June 2003. Organic fertilizer (alfalfa pellets, 112kg/ha/week) was used to increase primary production and inorganic fertilizers were added periodically to maintain a target nutrient ratio of 7:1 nitratenitrogen to total phosphorus (NO3-N: TP). Additional nutrients in the form of Lansy CW fish feed were added to three of the six ponds. The objective of this project was to determine the effect of a commercial fish diet on water quality. At the end of the culture season, there were significant differences between water chemistry parameters in the ponds; the feed treatments had higher levels of nitrogenous compounds and total phosphorus

Residual Stresses in Tungsten Thin Films for Single Photon Detectors

The residual stress in 20 nm thick tungsten films deposited on silicon substrate by dc magnetron sputtering is investigated. The sample was held in a continuous flow cryostat, which was capable of achieving temperatures as low as 8 K. The cryostat was mounted on a goniometer to enable the angle-dispersive x-ray diffraction measurements. X-ray diffraction was used to monitor the shift of the α-W {110} Bragg reflection at room temperature and 8 K. From the shift of the {110} Bragg reflection, the total residual stress was estimated at about 6.0 GPa. After applying corrections for the thermal stress in the film, the residual intrinsic stress is estimated at 5.8 GPa

Evaluation of Ceftaroline Activity against Heteroresistant Vancomycin-Intermediate Staphylococcus aureus and Vancomycin-Intermediate Methicillin-Resistant S. aureus Strains in an In Vitro Pharmacokinetic/Pharmacodynamic Model: Exploring the “Seesaw Effect”

A “seesaw effect” in methicillin-resistant Staphylococcus aureus (MRSA) has been demonstrated, whereby susceptibility to β-lactam antimicrobials increases as glyco- and lipopeptide susceptibility decreases. We investigated this effect by evaluating the activity of the anti-MRSA cephalosporin ceftaroline against isogenic pairs of MRSA strains with various susceptibilities to vancomycin in an in vitro pharmacokinetic/pharmacodynamic (PK/PD) model. The activities of ceftaroline at 600 mg every 12 h (q12h) (targeted free maximum concentration of drug in serum [fCmax], 15.2 μg/ml; half-life [t1/2], 2.3 h) and vancomycin at 1 g q12h (targeted fCmax, 18 μg/ml; t1/2, 6 h) were evaluated against 3 pairs of isogenic clinical strains of MRSA that developed increased MICs to vancomycin in patients while on therapy using a two-compartment hollow-fiber PK/PD model with a starting inoculum of ∼107 CFU/ml over a 96-h period. Bacterial killing and development of resistance were evaluated. Expression of penicillin-binding proteins (PBPs) 2 and 4 was evaluated by reverse transcription (RT)-PCR. The achieved pharmacokinetic parameters were 98 to 119% of the targeted values. Ceftaroline and vancomycin were bactericidal against 5/6 and 1/6 strains, respectively, at 96 h. Ceftaroline was more active against the mutant strains than the parent strains, with this difference being statistically significant for 2/3 strain pairs at 96 h. The level of PBP2 expression was 4.4× higher in the vancomycin-intermediate S. aureus (VISA) strain in 1/3 pairs. The levels of PBP2 and PBP4 expression were otherwise similar between the parent and mutant strains. These data support the seesaw hypothesis that ceftaroline, like traditional β-lactams, is more active against strains that are less susceptible to vancomycin even when the ceftaroline MICs are identical. Further research to explore these unique findings is warranted.This work was funded by an investigator-initiated grant from Forest Laboratories. M.J.R. is funded in part by NIH R21A1092055-01. We thank Abbott Laboratories for the use of the fluorescence polarization immunoassay analyzer for determination of vancomycin concentrations. We also thank Alexander Tomasz (The Rockefeller University, New York, NY) for providing strains JH-1 and JH-9. M.J.R. has received grant support, consulted for, or provided lectures for Astellas, Cubist, Forest, Pfizer, Novartis, and Rib-X. B.J.W., M.E.S., and G.W.K. have no potential conflicts of interest to declare

Hypoglycemia After Administration of Somatostatin Analog (SMS 201-995) in Metastatic Carcinoid

SMS 201-995 (Sandoz Pharmaceuticals. East Hanover NJ) is a synthetic peptide analog of native somatostatin that has been used to relieve .symptoms caused by neuroendocrine tumors. Reports have described an insulin suppressive effect of SMS 201-995 that results in elevations of blood glucose. We report a patient with a metastatic small bowel carcinoid and renal failure in whom mild symptomatic hypoglycemia occurred 30 to 60 minutes after SMS 201-995 administration. No increase in insulin or decreases in glucagon. Cortisol, or catecholamines were observed during these hypoglycemic episodes. Elevated levels of growth hormone fell gradually following SMS 201-995 administration and did not temporally correspond to the 30- to 60-minute nadir of blood glucose. However SMS 201-995 levels peaked during this 30- to 60-minute period. As clinical experience with this drug broadens, patients whose glucose control is dependent on counter-regulatory hormones should be monitored for the possibility of hypoglycemia

Reversing factor Xa inhibitors - clinical utility of andexanet alfa

Approximately half of patients started on an oral anticoagulant in the USA now receive one of the newer direct oral anticoagulants (DOACs). Although there is an approved reversal agent for the direct thrombin inhibitor dabigatran, a specific reversal agent for the anti-factor Xa (FXa) DOACs has yet to be licensed. Unlike the strategy to reverse the only oral direct thrombin inhibitor with idarucizumab, which is a humanized monoclonal antibody fragment, a different approach is necessary to design a single agent that can reverse multiple anti-FXa medications. Andexanet alfa is a FXa decoy designed to reverse all anticoagulants that act through this part of the coagulation cascade including anti-FXa DOACs, such as apixaban, edoxaban and rivaroxaban, and indirect FXa inhibitors such as low-molecular-weight heparins. This narrative reviews the development of andexanet alfa and explores its basic science, pharmacokinetics/pharmacodynamics, animal models, and human studies

COVID-19 and venous thromboembolism: A narrative review

COVID-19 (severe acute respiratory syndrome coronavirus 2 [SARS-CoV-2]) is associated with coagulopathy through numerous mechanisms. The reported incidence of venous thromboembolism (VTE) in hospitalized patients with COVID-19 has varied widely, and several meta-analyses have been performed to assess the overall prevalence of VTE. The novelty of this coronavirus strain along with its unique mechanisms for microvascular and macrovascular thrombosis has led to uncertainty as to how to diagnose, prevent, and treat thrombosis in patients affected by this virus. This review discusses the epidemiology and pathophysiology of thrombosis in the setting of SARS-CoV-2 infection along with an updated review on the preventative and treatment strategies for VTE associated with SARS-CoV-2 infection

Low agreement among reviewers evaluating the same NIH grant applications

Obtaining grant funding from the National Institutes of Health (NIH) is increasingly competitive, as funding success rates have declined over the past decade. To allocate relatively scarce funds, scientific peer reviewers must differentiate the very best applications from comparatively weaker ones. Despite the importance of this determination, little research has explored how reviewers assign ratings to the applications they review and whether there is consistency in the reviewers’ evaluation of the same application. Replicating all aspects of the NIH peer-review process, we examined 43 individual reviewers’ ratings and written critiques of the same group of 25 NIH grant applications. Results showed no agreement among reviewers regarding the quality of the applications in either their qualitative or quantitative evaluations. Although all reviewers received the same instructions on how to rate applications and format their written critiques, we also found no agreement in how reviewers “translated” a given number of strengths and weaknesses into a numeric rating. It appeared that the outcome of the grant review depended more on the reviewer to whom the grant was assigned than the research proposed in the grant. This research replicates the NIH peer-review process to examine in detail the qualitative and quantitative judgments of different reviewers examining the same application, and our results have broad relevance for scientific grant peer review