The after-effect of paclobutrazol on morphological characteristics of in vitro Narcissus poeticus ssp. radiiflorus plants

After different pre-culturing period (12, 23 or 34 days) on ½ MS medium with 1 mg l-1 paclobutrazol, 1 mg l-1 N6-benzyladenine and 0.1 mg l-1 1- naphthaleneacetic acid , 3 groups of Narcissus poeticus ssp. radiiflorus bulb scales were kept on the same medium without hormones. The results were evaluated monthly and the final one happened after 7 month. The best results were achieved due to the shortest pre-culturing period (12 days;  Group 1), with 4.9 bulblets and 4.54% hyperhydricity. The result of the second treatment (pre-culturing period of 23 days; Group 2) was not different significantly but the number of bigger bulblet were higher (4.54 bulblets). After the longest pre-culturing period (34 days; Group 3), the number of bulblets was low (3.68) and more hyperhydricity (18.18%) was detected. The highest number of roots (13.91) was observed in this groupvery likely due to the strong after-effect of paclobutrazol

Flowering performance of some Modern Rose Varieties in Hungary

A variety trial has been accomplished to study the flowering performance of some modern roses. Blooming time and blooming intensity were studied in Hungarian and Western European varieties. 120 Floribundas, polyanthas and climbings were observed. Our work shows that valuable Hungarian varieties can be found in all the three studied classes. The best Hungarian climbing roses at blooming intensity were `Futótűz', `Rozalia', ‘Sarolt and 'Szent Erzsébet emléke'; best floribundas were ‘Báthory István emléke', 'Munkács', 'Szent Margit emléke; and the best Hungarian polyanthas were `Csinszka', `Domokos PáI Péter emléke'. Some really good flowering Western-European rose varieties have also been found, the best ones were 'Clg. Gertrude Westphal' climbing, 'La Sevillana' floribunda and 'Beauty of New South Wells', 'Happy' polyanthus. 'La Sevillana' and 'Picasso' were in strong bloom for the longest time. In Hungary, the floribunda and polyantha classes had good flowering intensity to the almost the same extent, floribundas had stronger, and polyanthas had longer flowering waves, but the ever-blooming ability of the climbing roses was moderate in the dry midsummer

Flowering performance of some Modern Rose Varieties in Hungary

A variety trial has been accomplished to study the flowering performance of some modern roses. Blooming time and blooming intensity were studied in Hungarian and Western European varieties. 120 Floribundas, polyanthas and climbings were observed. Our work shows that valuable Hungarian varieties can be found in all the three studied classes. The best Hungarian climbing roses at blooming intensity were `Futótűz', `Rozalia', ‘Sarolt and 'Szent Erzsébet emléke'; best floribundas were ‘Báthory István emléke', 'Munkács', 'Szent Margit emléke; and the best Hungarian polyanthas were `Csinszka', `Domokos PáI Péter emléke'. Some really good flowering Western-European rose varieties have also been found, the best ones were 'Clg. Gertrude Westphal' climbing, 'La Sevillana' floribunda and 'Beauty of New South Wells', 'Happy' polyanthus. 'La Sevillana' and 'Picasso' were in strong bloom for the longest time. In Hungary, the floribunda and polyantha classes had good flowering intensity to the almost the same extent, floribundas had stronger, and polyanthas had longer flowering waves, but the ever-blooming ability of the climbing roses was moderate in the dry midsummer

Colour stability of the flowers in some modern rose varieties in Hungary

A variety trial has been carried out to study the colour changing — colour stability of the flowers of some modern roses, especially of Hungarian varieties. 100 floribundas and polyanthas were observed. Colours of the petal surface were examined at three fenological stages of the flowers: at the bud, at the young open flowers and at the aged open flower stages. The colour difference was described in HLS standard. Our work shows that valuable Hungarian varieties can be found in both studied classes, but more floribundas bred in Hungary had good colour stability than polyanthas. The best Hungarian floribundas were 'Pest' and 'Remenyik Sándor emléke', although 'Regen', `Szent Lász1ó em­léke' and 'Szabó Dezső emléke' were quite good. The best Western-European floribunda rose was 'Perneille Poulsen'. Their colour stability was reliable in different situations. Colour changing of the two best varieties was almost unperceivable from the bud to the young petals and from the young to the aged petals. In the polyantha class there was only one perfect variety: the Czech `Cs1 Cerveny Kriz'. The 'Fairy Damsel' was almost excellent as well. The best Hungarian polyanthas were `Domokos Pál Péter emléke' and 'Savaria'

In vitro propagation of 'Echo' cultivars of Eustoma grandiflorum (Raf.) Shinn.

Eustoma grandiflorum (Raf.) Shinn. 'Echo' Fl cultivars ('Echo White', 'Echo Rose', 'Echo Blue', 'Echo Blue Picotee') were used and multiplication of shoots was evaluated on Murashige and Skoog (1962) basal medium with 11 g/1 agar-agar and 20 g/1 sucrose. To test the effect of BA different concentrations were added: 0.10, 0.25 mg/1 and a culture medium without BA. Differentiation of roots was examined on Jámbor-Benczúr and Marta (1990) basal medium with the same concentration of agar-agar and sucrose. To examine the effect on rooting, various concentrations of NAA were used: 0.5, 1.0, 2.0, 3.0 mg/l. The pH was adjusted to 5.6 in every case using KOH. We studied the after-effect of different concentrations of BA during the acclimatisation. During the multiplication, the cultivar 'Echo White' formed the most shoots and the smallest leaves on the medium with 0.10 mg/1 BA. Fortunately, in the case of this cultivar, the number of shoots was reduced and the length of leaves was increased succesfully on the medium without BA. The other three cultivars developed the longest leaves on the medium containing 0.10 mg/1 BA. Sometimes not only shoot regeneration but spontaneous rooting was observed during the multiplication. Examining the rooting, the highest percent of roots was found on the medium with 1.0 mg/1 NAA, and the cultivar 'Echo Rose' formed the most roots on this medium. Higher concentration (2.0 and 3.0 mg/1) of NAA already reduced the number of roots in all of the cultivars. During the acclimatisation, the percentage of survival was 76.3% and the tallest plants with the longest leaves were found on the multiplication medium with 0.25 mg/1 BA. 'Echo Blue Picotee' gave the best results with the tallest pieces and longest leaves on this medium

Results with the establishment of in vitro culture of Leucojum aestivum

Leucojum aestivum is a native, protected ornamental and medicinal plant in Hungary and in Ukraine too. The aim of our work was to establish in vitro cultures of this bulbous plant. Prior to surface sterilisation the old leaves and roots were dissected from the bulbs and they were stored in a refrigerator (2-3°C) for different periods (1 week for the first starting experiment and 5 weeks for the second one). After sterilisation, bulbs, bulb scales and leaves of the bulbs were placed on Murashige and Skoog's (1962) medium with 1 mg/1 benzyl-adenine (BA) and 0,1 mg/1 naphthalene acetic acid (NAA). At the first starting experiment 81,3%, and at the second one 92,3% of the explants turned to be sterile. Bulblets and roots were developed on the explants in the case of using bulb plates together with bulb scales and leaves as inoculua. The best result was achieved after 5 weeks chilling and it was possible to gain little bulbs from the bulb leaves too

In vitro propagation of 'Echo' cultivars of Eustoma grandiflorum (Raf.) Shinn.

Eustoma grandiflorum (Raf.) Shinn. 'Echo' Fl cultivars ('Echo White', 'Echo Rose', 'Echo Blue', 'Echo Blue Picotee') were used and multiplication of shoots was evaluated on Murashige and Skoog (1962) basal medium with 11 g/1 agar-agar and 20 g/1 sucrose. To test the effect of BA different concentrations were added: 0.10, 0.25 mg/1 and a culture medium without BA. Differentiation of roots was examined on Jámbor-Benczúr and Marta (1990) basal medium with the same concentration of agar-agar and sucrose. To examine the effect on rooting, various concentrations of NAA were used: 0.5, 1.0, 2.0, 3.0 mg/l. The pH was adjusted to 5.6 in every case using KOH. We studied the after-effect of different concentrations of BA during the acclimatisation. During the multiplication, the cultivar 'Echo White' formed the most shoots and the smallest leaves on the medium with 0.10 mg/1 BA. Fortunately, in the case of this cultivar, the number of shoots was reduced and the length of leaves was increased succesfully on the medium without BA. The other three cultivars developed the longest leaves on the medium containing 0.10 mg/1 BA. Sometimes not only shoot regeneration but spontaneous rooting was observed during the multiplication. Examining the rooting, the highest percent of roots was found on the medium with 1.0 mg/1 NAA, and the cultivar 'Echo Rose' formed the most roots on this medium. Higher concentration (2.0 and 3.0 mg/1) of NAA already reduced the number of roots in all of the cultivars. During the acclimatisation, the percentage of survival was 76.3% and the tallest plants with the longest leaves were found on the multiplication medium with 0.25 mg/1 BA. 'Echo Blue Picotee' gave the best results with the tallest pieces and longest leaves on this medium

Results with the establishment of in vitro culture of Leucojum aestivum

Leucojum aestivum is a native, protected ornamental and medicinal plant in Hungary and in Ukraine too. The aim of our work was to establish in vitro cultures of this bulbous plant. Prior to surface sterilisation the old leaves and roots were dissected from the bulbs and they were stored in a refrigerator (2-3°C) for different periods (1 week for the first starting experiment and 5 weeks for the second one). After sterilisation, bulbs, bulb scales and leaves of the bulbs were placed on Murashige and Skoog's (1962) medium with 1 mg/1 benzyl-adenine (BA) and 0,1 mg/1 naphthalene acetic acid (NAA). At the first starting experiment 81,3%, and at the second one 92,3% of the explants turned to be sterile. Bulblets and roots were developed on the explants in the case of using bulb plates together with bulb scales and leaves as inoculua. The best result was achieved after 5 weeks chilling and it was possible to gain little bulbs from the bulb leaves too

Knot formation by Pseudomonas syringae subsp. savastanoi on the in vitro shoots of Sorbus redliana

Two strains of Pseudomonas syringae subsp. savastanoi were isolated from Forsythia sp. and Nerium oleander in Hungary in 1997. The effects of growth regulators produced by the bacteria were studied in different experiments. The strains were co-cultured with Sorbus redliana in vitro shoots without being in contact with the plant on solid media. Further culture filtrates in different concentrations were added to the culture medium. The growth regulators presented in the agar caused knot formation on the shoots and on the leaves in both kinds of culture. There were significant differences in the cultural and physiological characters, auxin and cytokinin activity of the strains of different origin. &nbsp

The effects of growth regulators in proliferation of Sorbus redliana 'Burokvölgy'

The Hungarian cultivar Sorbus redliana 'Burokvölgy' was proliferated on Murashige and Skoog (MS, 1962) medium with half-strength macroelements and 100 mg/1 meso-inositol, 20 g/1 sucrose, 11 g/1 agar-agar. Different combinations of kinetin (KIN), metatopolin (mT), benzyladenine (BA), benzyladenine-ribosid (BAR) and indolebutiric acid (IBA) were tested, and pH was adjusted to 5.6 every case using KOH. The cultures were incubated at 20-24 °C in 8/16 hours dark/light photoperiod for 50-52 days. The main aim of our research was to find the optimal growth regulator and its optimum concentration. Purthermore, to determine the chlorophyll contents of the in vitro propagated plants' leaves. During the proliferation, the highest number of shoots were observed in the case of using BA + IBA, and on the medium containing 0.75 mg/I BA + 0.05 mg/1 IBA 8.93 shoots were found. The addition of KIN + IBA decreased the number of shoots and increased the sizes of leaves — the widest (11.2 mm) and longest (17.8 mm) leaves were obtained on the medium containing 1.00 mg/I KIN + 0.05 mg/1 IBA. The longest shoots (36.46 mm) were found in the case of applying 0.75 mg/1 BAR + 0.05 mg/I IBA. The BA + KIN + IBA combination resulted the shortest shoots. Sometimes not only shoot regeneration but spontaneous rooting was observed during the multiplication. The highest chlorophyll content (1.569 mg/g total chlorophyll, 1.132 mg/g chlorophyll-a, 0.437 mg/g chlorophyll-b) was obtained in the presence of 1.0 mg/I KIN + 0.05 mg/1 IBA