45 research outputs found
Perception of nonnative tonal contrasts by Mandarin-English and English-Mandarin sequential bilinguals
This study examined the role of acquisition order and crosslinguistic similarity in influencing transfer at the initial stage of perceptually acquiring a tonal third language (L3). Perception of tones in Yoruba and Thai was tested in adult sequential bilinguals representing three different first (L1) and second language (L2) backgrounds: L1 Mandarin-L2 English (MEBs), L1 English-L2 Mandarin (EMBs), and L1 English-L2 intonational/non-tonal (EIBs). MEBs outperformed EMBs and EIBs in discriminating L3 tonal contrasts in both languages, while EMBs showed a small advantage over EIBs on Yoruba. All groups showed better overall discrimination in Thai than Yoruba, but group differences were more robust in Yoruba. MEBsā and EMBsā poor discrimination of certain L3 contrasts was further reflected in the L3 tones being perceived as similar to the same Mandarin tone; however, EIBs, with no knowledge of Mandarin, showed many of the same similarity judgments. These findings thus suggest that L1 tonal experience has a particularly facilitative effect in L3 tone perception, but there is also a facilitative effect of L2 tonal experience. Further, crosslinguistic perceptual similarity between L1/L2 and L3 tones, as well as acoustic similarity between different L3 tones, play a significant role at this early stage of L3 tone acquisition.Published versio
ParĆ¢metros genĆ©ticos de caracterĆsticas reprodutivas de touros e vacas Gir leiteiro
Dependence of the optical cross section of interface states on the photon energy at Si-SiO2 structures
Ex vivo differentiation of human bone marrow-derived stem cells into neuronal cell-like lineages
Adeeb Al-Zoubi,1,2 Feras Altwal,3 Farah Khalifeh,2 Jamil Hermas,4 Ziad Al-Zoubi,5 Emad Jafar,5 Mohammed El-Khateeb,6,7 1Department of Surgery, University of Illinois College of Medicine at Peoria, Peoria, IL, USA; 2Stem Cells of Arabia, Amman, Jordan; 3Department of Neuroscience, School of Graduate and Postdoctoral Studies, Rosalind Franklin University of Medicine and Science, North Chicago, IL, USA; 4Stem Cell Division, Al-Yamama Company, 5Jordan Orthopedic and Spinal Center, 6National Center for Diabetes, Endocrinology and Genetics, 7Department of Pathology, Faculty of Medicine, University of Jordan, Amman, Jordan Background: Methods to obtain safe and practical populations of stem cells (SCs) at a clinical grade that are able to differentiate into neuronal cell lineages are yet to be developed. In a previous study, we showed that mouse bone marrow-derived SCs (BM-SCs) differentiated into neuronal cell-like lineages when put in a neuronal-like environment, which is a special media supplemented with the necessary growth factors needed for the differentiation of SCs into neuronal cell-like lineages. Aim: In this study, we aim to assess the potentials of adult human CD34+ and CD133+ SCs to differentiate into neuronal cell-like lineages ex vivo when placed in a neuronal-like microenvironment. Methods: The neuronal-like microenvironment was created by culturing cells in nonhematopoietic expansion media (NHEM) supplemented with growth factors that favor differentiation into neuronal cell lineages (low-affinity nerve growth factor [LNGF], mouse spinal cord extract [mSpE], or both). Cultured cells were assessed for neuronal differentiation by cell morphologies and by expression of GFAP. Results: Our results show that culturing unpurified human BM-derived mononuclear cells (hBM-MNCs) in NHEM+LNGF+mSpE did not lead to neuronal differentiation. In contrast, culturing of purified CD34+ hBM-SCs in NHEM+LNGF+mSpE favored their differentiation into astrocyte-like cells, whereas culturing of purified CD133+ hBM-SCs in the same media favored their differentiation into neuronal-like cells. Interestingly, coculturing of CD34+ and CD133+ hBM-SCs in the same media enhanced the differentiation into astrocyte-like cells and neuronal-like cells, in addition to oligodendrocyte-like cells. Conclusion: These results suggest that a mixture of purified CD34+ and CD133+ cells may enhance the differentiation into neuronal cell-like lineages and give broader neuronal cell lineages than when each of these cell types is cultured alone. This method opens the window for the utilization of specific populations of hBM-SCs to be delivered in a purified form for the potential treatment of neurodegenerative diseases in the future. Keywords: cell therapy, neurodegenerative disease, neurons, astrocyes, CD34+, CD133+, MAC
Source(s) of syntactic cross-linguistic influence (CLI): The case of L3 acquisition of English possessives by MazandaraniāPersian bilinguals
Iron(II) Complexes with Scorpiand-Like Macrocyclic Polyamines: Kinetico-Mechanistic Aspects of Complex Formation and Oxidative Dehydrogenation of Coordinated Amines
The
FeĀ(II) coordination chemistry of a pyridinophane <i>tren</i>-derived scorpiand type ligand containing a pyridine ring in the
pendant arm is explored by potentiometry, X-ray, NMR, and kinetics
methods. Equilibrium studies in water show the formation of a stable
[FeL]<sup>2+</sup> complex that converts to monoprotonated and monohydroxylated
species when the pH is changed. A [FeĀ(H<sub>ā2</sub>L)]<sup>2+</sup> complex containing an hexacoordinated dehydrogenated ligand
has been isolated, and its crystal structure shows the formation of
an imine bond involving the aliphatic nitrogen of the pendant arm.
This complex is low spin FeĀ(II) both in the solid state and in solution,
as revealed by the FeāN bond lengths and by the NMR spectra,
respectively. The formation rate of [FeĀ(H<sub>ā2</sub>L)]<sup>2+</sup> in aqueous solutions containing Fe<sup>2+</sup> and L (1:1
molar ratio) is strongly dependent on the pH, the process being completed
in times that range from months in acid solutions to hours in basic
conditions. However, detailed kinetic studies show that those differences
are caused, at least in part, by the effect of pH on the rate of formation
of the unoxidized [FeL]<sup>2+</sup> complex. In this sense, the protonation
of the donor atoms in the pendant arm of the scorpiand ligand leads
to the formation of protonated species resistant to oxidative dehydrogenation.
Complementary studies in acetonitrile solution indicate that the initial
stage in the oxidative dehydrogenation process is the oxidation of
the starting complex to form a [FeL]<sup>3+</sup> complex, which then
undergoes disproportionation into [FeĀ(H<sub>ā2</sub>L)]<sup>2+</sup> and [FeL]<sup>2+</sup>. Experiments starting with FeĀ(III)
have allowed us to determine that disproportionation occurs with first
order kinetics both in water and acetonitrile solutions. However,
whereas a significant acceleration is observed in water when the pH
is increased, no effect of the addition of acid or base on the rate
of disproportionation is observed in acetonitrile. Oxidative dehydrogenation
of the FeĀ(II) complex formed in experiments starting with an FeĀ(III)
salt is slower than that occurring when an FeĀ(II) salt is used, an
observation that can be explained in terms of the formation of two
different FeĀ(III) complexes, one of them with a structure unable to
evolve directly toward the product of oxidative dehydrogenation