69,919 research outputs found
Model of Transcriptional Activation by MarA in Escherichia coli
We have developed a mathematical model of transcriptional activation by MarA
in Escherichia coli, and used the model to analyze measurements of
MarA-dependent activity of the marRAB, sodA, and micF promoters in mar-rob-
cells. The model rationalizes an unexpected poor correlation between the
mid-point of in vivo promoter activity profiles and in vitro equilibrium
constants for MarA binding to promoter sequences. Analysis of the promoter
activity data using the model yielded the following predictions regarding
activation mechanisms: (1) MarA activation of the marRAB, sodA, and micF
promoters involves a net acceleration of the kinetics of transitions after RNA
polymerase binding, up to and including promoter escape and message elongation;
(2) RNA polymerase binds to these promoters with nearly unit occupancy in the
absence of MarA, making recruitment of polymerase an insignificant factor in
activation of these promoters; and (3) instead of recruitment, activation of
the micF promoter might involve a repulsion of polymerase combined with a large
acceleration of the kinetics of polymerase activity. These predictions are
consistent with published chromatin immunoprecipitation assays of interactions
between polymerase and the E. coli chromosome. A lack of recruitment in
transcriptional activation represents an exception to the textbook description
of activation of bacterial sigma-70 promoters. However, use of accelerated
polymerase kinetics instead of recruitment might confer a competitive advantage
to E. coli by decreasing latency in gene regulation.Comment: 30 pages, 2 figure
Extending electron orbital precession to the molecular case: Can orbital alignment be used to observe wavepacket dynamics?
The complexity of ultrafast molecular photoionization presents an obstacle to
the modelling of pump-probe experiments. Here, a simple optimized model of
atomic rubidium is combined with a molecular dynamics model to predict
quantitatively the results of a pump-probe experiment in which long range
rubidium dimers are first excited, then ionized after a variable delay. The
method is illustrated by the outline of two proposed feasible experiments and
the calculation of their outcomes. Both of these proposals use Feshbach 87Rb2
molecules. We show that long-range molecular pump-probe experiments should
observe spin-orbit precession given a suitable pump-pulse, and that the
associated high-frequency beat signal in the ionization probability decays
after a few tens of picoseconds. If the molecule was to be excited to only a
single fine structure state state, then a low-frequency oscillation in the
internuclear separation would be detectable through the timedependent
ionization cross section, giving a mechanism that would enable observation of
coherent vibrational motion in this molecule.Comment: 9 pages, 10 figures, PRA submissio
Charge and Spin Density Waves observed through their spatial fluctuations by coherent and simultaneous X-ray diffraction
Spatial uctuations of spin density wave (SDW) and charge density wave (CDW)
in chromium have been compared by combining coherent and simultaneous X-ray
diffraction experiments. Despite their close relationship, spatial fluctuations
of the spin and of the charge density waves display a very different behavior:
the satellite reflection associated to the charge density displays speckles
while the spin one displays an impressive long-range order. This observation is
hardly compatible with the commonly accepted magneto-elastic origin of CDW in
chromium and is more consistent with a purely electronic scenario where CDW is
the second harmonic of SDW. A BCS model taking into account a second order
nesting predicts correctly the existence of a CDW and explains why the CDW is
more sensitive to punctual defects.Comment: 4 pages, 4 figures; Accepted in Phys. Rev.
Decoherence and entropy of primordial fluctuations II. The entropy budget
We calculate the entropy of adiabatic perturbations associated with a
truncation of the hierarchy of Green functions at the first non trivial level,
i.e. in a self-consistent Gaussian approximation. We give the equation
governing the entropy growth and discuss its phenomenology. It is parameterized
by two model-dependent kernels. We then examine two particular inflationary
models, one with isocurvature perturbations, the other with corrections due to
loops of matter fields. In the first model the entropy grows rapidely, while in
the second the state remains pure (at one loop).Comment: 28 page
Beef Cattle Production and Management Practices and Implications for Educators
Beef producers need to continually incorporate new information and adopt new technology to effectively manage production costs. Oklahoma State University began a Master Cattleman program with this need in mind. Understanding technology adoption by producers requires identifying current management practices. Data from a survey developed as part of the Master Cattleman program document current practices. Management practices were examined for two groups; producers with smaller herds who are less dependent on the beef enterprise for family income, and producers with larger herds who are more dependent on beef. Results clearly show that size and dependence on the beef enterprise matters when considering a broad spectrum of beef management practices.Livestock Production/Industries,
Higgs Boson Exempt No-Scale Supersymmetry and its Collider and Cosmology Implications
One of the most straightforward ways to address the flavor problem of
low-energy supersymmetry is to arrange for the scalar soft terms to vanish
simultaneously at a scale much larger than the electroweak scale. This
occurs naturally in a number of scenarios, such as no-scale models, gaugino
mediation, and several models with strong conformal dynamics. Unfortunately,
the most basic version of this approach that incorporates gaugino mass
unification and zero scalar masses at the grand unification scale is not
compatible with collider and dark matter constraints. However, experimental
constraints can be satisfied if we exempt the Higgs bosons from flowing to zero
mass value at the high scale. We survey the theoretical constructions that
allow this, and investigate the collider and dark matter consequences. A
generic feature is that the sleptons are relatively light. Because of this,
these models frequently give a significant contribution to the anomalous
magnetic moment of the muon, and neutralino-slepton coannihilation can play an
important role in obtaining an acceptable dark matter relic density.
Furthermore, the light sleptons give rise to a large multiplicity of lepton
events at colliders, including a potentially suggestive clean trilepton signal
at the Tevatron, and a substantial four lepton signature at the LHC.Comment: 36 pages, 16 figure
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