Uptake and translocation of 134Cs by maize roots as affected by heterogeneous distribution of 134Cs

Structure-induced non-uniform water flow induces a heterogeneous distribution of surface-applied radionuclides in the soil profile. This study was conducted to assess the amount of 134Cs which can be taken up by a single root growing in an area enriched in 134Cs relative to the total amount of 134Cs that can be taken up by the whole root system growing in an area homogeneously contaminated with 134Cs. A split-root experiment was used to simulate the heterogeneous distribution of 134Cs and roots. Seedlings of maize (Zea mays L. cv Corso) were grown for 14days in solution culture and then transferred to a two-compartment pot system, where a single root was grown in a 134Cs contaminated compartment while the rest of the root system was grown in an uncontaminated compartment. Plants with the whole root system growing in a solution contaminated with 134Cs were used as control. We tested the effect of the competition between Cs and K on the uptake and translocation of 134Cs by using two K concentrations, 0.2 and 1.05mM. At the K concentration of the nutrient solution of 0.2mM the single root representing 21% of the total root weight was able to take up 47% of the 134Cs taken up by the entire root system, while at 1.05mM the single root, representing 15% of the total root weight, took up 15% of the 134Cs taken up by the entire root system. The translocation of 134Cs from the root to the shoots did not depend on the external K concentration in the nutrient solution, but it was lower in the split root treatment than in the control treatment at both K concentration

Effect of compost and soil properties on the availability of compost phosphate for white clover ( Trifolium repens L.)

Wide variation in results exists in the literature on the effectiveness of composts to sustain the phosphorus (P) nutrition of crops. The aim of this work was to assess the importance of some soil and composts properties on the utilization of compost-P by white clover (Trifolium repens L.). This study was carried out with samples collected from four composts made from solid kitchen and garden wastes, and with two soil samples taken from the A horizon of a P-rich sandy acidic Dystrochrept and of a P-limited clayey calcareous Eutrochrept. Changes in the amount of inorganic P (Pi) isotopically exchangeable within 1 min (E1min) were measured during 32 weeks in incubated soil-composts or soil-KH2PO4mixtures where P sources had been added at the rate of 50 mg P kg−1 soil. Uptake of compost-P or KH2PO4-P by white clover was measured on the same amended soils during 16 weeks. In both soils, the application of composts resulted after 32 weeks of incubation in E1min values ranging between those observed in the control without P and those observed in the KH2PO4treatment, i.e., in values ranging between 4.2 and 5.9 mg P kg−1 in the sandy acidic soil and between from 1.6 to 4.3 mg P kg−1 in the clayey calcareous soil. The total coefficient of utilization of compost-P (CU-P) by white clover reached values in both soils for the four composts ranging between 6.5% and 11.6% of the added P while in the presence of KH2PO4 the CU-P reached values ranging between 14.5% in the clayey calcareous soil and 18.5% in the sandy acidic soil. Results obtained in the sandy acidic soil suggest, that white clover initially used a fraction of the rapidly exchangeable compost P, while at a latter stage plant roots enhanced the mineralisation of compost organic P and took up a fraction of the mineralized P. These relations were not observed in the clayey calcareous soil probably because of its high sorbing capacity for P. In the sandy acidic soil, composts application increased the uptake of soil P by the plant from 31.4 mg P kg−1 soil in the control without P to values ranging between 37.9 to 42.7 mg P kg−1 soil in the presence of composts. This indirect effect was related to a general improvement of plant growth conditions in this soil induced by compost addition (from 9.9 g DM kg−1 soil in the control without P to values ranging between 14.0 to 16.1 g DM kg−1 soil in the presence of composts) and/or to the release of Al- or Fe bound soil P to the solution due to soil pH increase following compost application. Finally the total coefficient of utilization of P (CU-P) derived from KH2PO4 and composts was related to the total amount of N exported by white clover in the P-limited clayey calcareous soil but not in the P-rich sandy acidic soil. This suggests that in a soil where N2 biological fixation is limited by low P availability, the CU-P of a compost by white clover is not only related to the forms of P present in the compost but also to its effect on N nutrition. However, it is not clear whether this improved N nutrition was due to compost mineralisation, or to an indirect compost effect on the N2 biological fixatio

Soil isotopically exchangeable phosphorus : a comparison between E and L values

This study was designed to explain the apparent discrepancies often reported in the literature between E and L values, two parameters obtained from isotopic exchange experiments and commonly used to quantify available soil phosphate. The E and L values of the surface horizons of 10 soils from tropical, mediterranean, and temperate regions were determined. The L value was measured from a 13-wk pot experiment with common bentgrass (#Agrostis capillaris$ L.) where the available soil P was labeled with 32PO4 ions in the presence of a carrier (25-50 mg 31P/kg soil). To determine the E value, the isotopic exchange kinetic experiment was carried out on each soil. Carrier-free 32PO4 was added to the soil-solution system at a steady state and the quantity of isotopically exchangeable soil phosphate at time t, E(t), was calculated from the kinetic equation describing the decrease of radioactivity in solution with time. Results showed that L values determined after 13 wk were not significantly different from E(t) values extrapolated to the same period (t = 131 040 min). It was concluded that the L value is a particular point of the kinetic equation and isotopically exchangeable phosphate is the available P for common bentgrass. A strict equality between E(13 wk) and L values was not, however, reached for all samples. Possible causes for the differences were : an overestimation of the water-soluble phosphate due to the presence of silica and disturbance of the steady state following a too large uptake of phosphate by the crop or the application of too large quantities of carrier compared with the initial quantity of exchangeable soil phosphate. (Résumé d'auteur

The effects of water regime on phosphorus responses of rainfed lowland rice cultivars

Background and Aims Soil phosphorus (P) solubility declines sharply when a flooded soil drains, and an important component of rice (Oryza sativa) adaptation to rainfed lowland environments is the ability to absorb and utilize P under such conditions. The aim of this study was to test the hypothesis that rice cultivars differ in their P responses between water regimes because P uptake mechanisms differ. Methods Six lowland rice cultivars (three considered tolerant of low P soils, three sensitive) were grown in a factorial experiment with three water regimes (flooded, moist and flooded-then-moist) and four soil P levels, and growth and P uptake were measured. Small volumes of soil were used to maximize inter-root competition and uptake per unit root surface. The results were compared with the predictions of a model allowing for the effects of water regime on P solubility and diffusion. Key Results The plants were P stressed but not water stressed in all the water regimes at all P levels except the higher P additions in the flooded soil. The cultivar rankings scarcely differed between the water regimes and P additions. In all the treatments, the soil P concentrations required to explain the measured uptake were several times the concentration of freely available P in the soil. Conclusions The cultivar rankings were driven more by differences in growth habit than specific P uptake mechanisms, so the hypothesis cannot be corroborated with these data. Evidently all the plants could tap sparingly soluble forms of P by releasing a solubilizing agent or producing a greater root length than measured, or both. However, any cultivar differences in this were not apparent in greater net P uptake, possibly because the restricted rooting volume meant that additional P uptake could not be converted into new root growth to explore new soil volume

A dual isotopic approach using radioactive phosphorus and the isotopic composition of oxygen associated to phosphorus to understand plant reaction to a change in P nutrition

Abstract Background Changing the phosphorus (P) nutrition leads to changes in plant metabolism. The aim of this study was to investigate how these changes are reflected in the distribution of 33P and the isotopic composition of oxygen associated to P (δ18OP) in different plant parts of soybean (Glycine max cv. Toliman). Two P pools were extracted sequentially with 0.3 M trichloroacetic acid (TCA P) and 10 M nitric acid (HNO3; residual P). Results The δ18OP of TCA P in the old leaves of the − P plants (23.8‰) significantly decreased compared to the + P plants (27.4‰). The 33P data point to an enhanced mobilisation of P from residual P in the old leaves of the − P plants compared to the + P plants. Conclusions Omitting P for 10 days lead to a translocation of P from source to sink organs in soybeans. This was accompanied by a significant lowering of the δ18OP of TCA P in the source organs due to the enzymatic hydrolysis of organic P. Combining 33P and δ18OP can provide useful insights in plant responses to P omission at an early stage

Phosphorus Transformations in an Oxisol under contrasting land-use systems: The role of the soil microbial biomass

It is generally assumed that phosphorus (P) availability for plant growth on highly weathered and P-deficient tropical soils may depend more on biologically mediated organic P (Po) turnover processes than on the release of adsorbed inorganic P (Pi). However, experimental evidence showing the linkages between Po, microbial activity, P cycling and soil P availability is scarce. To test whether land-use systems with higher soil Po are characterized by greater soil biological activity and increased P mineralization, we analyzed the partitioning of P among various organic and inorganic P fractions in soils of contrasting agricultural land-use systems and related it to biological soil properties. Isotopic labeling was used to obtain information on the turnover of P held in the microbial biomass. Soil samples were taken from grass-legume pasture (GL), continuous rice (CR) and native savanna (SAV) which served as reference. In agreement with estimated P budgets (+277, +70 and 0 kg P ha−1 for CR, GL and SAV, respectively), available P estimated using Bray-2 and resin extraction declined in the order CR > GL > SAV. Increases in Bray-2 and resin Pi were greater in CR than GL relative to total soil P increase. Organic P fractions were significantly less affected by P inputs than inorganic fractions, but were a more important sink in GL than CR soils. Extractable microbial P (Pchl) was slightly higher in GL (6.6 mg P kg−1) than SAV soils (5.4 mg P kg−1), and significantly lowest in CR (2.6 mg P kg−1). Two days after labeling the soil with carrier free 33P, 25, 10 and 2% of the added 33P were found in Pchl in GL, SAV and CR soils, respectively, suggesting a high and rapid microbial P turnover that was highest in GL soils. Indicators of P mineralization were higher in GL than CR soils, suggesting a greater transformation potential to render Po available. Legume-based pastures (GL) can be considered as an important land-use option as they stimulate P cycling. However, it remains to be investigated whether crops planted in pasture-crop rotations could benefit from the enhanced Po cycling in grass-legume soils. Furthermore, there is need to develop and test a direct method to quantify Po mineralization in these system

3D Face Recognition with Sparse Spherical Representations

This paper addresses the problem of 3D face recognition using simultaneous sparse approximations on the sphere. The 3D face point clouds are first aligned with a novel and fully automated registration process. They are then represented as signals on the 2D sphere in order to preserve depth and geometry information. Next, we implement a dimensionality reduction process with simultaneous sparse approximations and subspace projection. It permits to represent each 3D face by only a few spherical functions that are able to capture the salient facial characteristics, and hence to preserve the discriminant facial information. We eventually perform recognition by effective matching in the reduced space, where Linear Discriminant Analysis can be further activated for improved recognition performance. The 3D face recognition algorithm is evaluated on the FRGC v.1.0 data set, where it is shown to outperform classical state-of-the-art solutions that work with depth images