47 research outputs found
Expression of human growth hormone in the milk of transgenic rabbits with transgene mapped to the telomere region of chromosome 7q
Full text linkSequence of the rabbit beta-casein cDNA: comparison with other casein cDNA sequences
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A distal region, hypersensitive to DNase I, plays a key role in regulating rabbit whey acidic protein gene expression.
No full textThe aim of the present study was to identify the functional domains of the upstream region of the rabbit whey acidic protein (WAP) gene, which has been used with considerable efficacy to target the expression of several foreign genes to the mammary gland. We have shown that this region exhibits three sites hypersensitive to DNase I digestion in the lactating mammary gland, and that all three sites harbour elements which can bind to Stat5 in vitro in bandshift assays. However, not all hypersensitive regions are detected at all stages from pregnancy to weaning, and the level of activated Stat5 detected in the rabbit mammary gland is low except during lactation. We have studied the role of the distal site, which is only detected during lactation, in further detail. It is located within a 849 bp region that is required to induce a strong expression of the chloramphenicol acetyltransferase reporter gene in transfected mammary cells. Taken together, these results suggest that this region, centred around a Stat5-binding site and surrounded by a variable chromatin structure during the pregnancy-lactation cycle, may play a key role in regulating the expression of this gene in vivo. Furthermore, this distal region exhibits sequence similarity with a region located around 3 kb upstream of the mouse WAP gene. The existence of such a distal region in the mouse WAP gene may explain the differences in expression between 4.1 and 2.1 kb mouse WAP constructs
Association between litter size and the k-casein genotype in the INRA rabbit lines
Full text link[EN] The reproductive traits of 276 rabbit does belonging to an intercross generation of two synthetic INRAlines, which differed by their genotypes at the k-casein locus being either AA or AB, were recorded at birth andat weaning for a total of 743 litters. They were the daughters of 77 dams whose k-casein genotype was ABand 18 sires whose genotype was AA. A significant association between the k-casein genotype andreproductive traits at birth was found in favour of the AB females, with an increase in litter size (+0.56;P<0.009) as well as in litter weight (+27 g; P<0.023). After standardisation of the litter size at birth, thegenotype of the dam was found to have no effect on weight gain and the viability of the young between birthand weaning or on the litter weight at weaning. Work is now in progress to explain this association.This work was financially supported by Balaton F33/98 and by OTKA T34767 to B. Zs. We acknowledge W. Brand-Williams for the English revision of the manuscript.Bolet, G.; Devinoy, E.; Viràg, G.; Harsányi, I.; Bosze, Z. (2007). Association between litter size and the k-casein genotype in the INRA rabbit lines. World Rabbit Science. 15(3). doi:10.4995/wrs.2007.595SWORD15
