703 research outputs found

    PROJECTED COSTS FOR SELECTED LOUISIANA VEGETABLE CROPS - 2001 SEASON

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    Cost budgets are reported for 20 vegetable crops, with a total of 38 combinations of crop, machinery size, and market channel.Farm Management,

    PROJECTED COSTS FOR SELECTED LOUISIANA VEGETABLE CROPS - 1997 SEASON

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    Information on cultural practices, machinery complements, etc. was obtained from a total of 48 commercial growers. Interviews were confined to growers with at least one acre of a particular vegetable crop. Individual acreage of growers interviewed ranged from one for some vegetable crops to several hundred in the case of sweet potatoes. Cost budgets are reported for 21 vegetable crops, with a total of 39 combinations of crop, machinery size, and market channel.Farm Management,

    Electron microscopic analysis of rotavirus assembly-replication intermediates

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    AbstractRotaviruses (RVs) replicate their segmented, double-stranded RNA genomes in tandem with early virion assembly. In this study, we sought to gain insight into the ultrastructure of RV assembly-replication intermediates (RIs) using transmission electron microscopy (EM). Specifically, we examined a replicase-competent, subcellular fraction that contains all known RV RIs. Three never-before-seen complexes were visualized in this fraction. Using in vitro reconstitution, we showed that ~15-nm doughnut-shaped proteins in strings were nonstructural protein 2 (NSP2) bound to viral RNA transcripts. Moreover, using immunoaffinity-capture EM, we revealed that ~20-nm pebble-shaped complexes contain the viral RNA polymerase (VP1) and RNA capping enzyme (VP3). Finally, using a gel purification method, we demonstrated that ~30–70-nm electron-dense, particle-shaped complexes represent replicase-competent core RIs, containing VP1, VP3, and NSP2 as well as capsid proteins VP2 and VP6. The results of this study raise new questions about the interactions among viral proteins and RNA during the concerted assembly–replicase process
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