7 research outputs found
Analysis of Emergency Situations When Working with Pathogenic Biological Agents within the Framework of Risk-Oriented Approach
The aim β based on the analysis of accidents when working with pathogenic biological agents (PBA) of pathogenicity groups IβII, draw conclusions about the causes of their occurrence and formulate recommendations for improving biological safety measures to reduce the risk of accidents.Materials and methods. The subject of the study was the data on accidents that happened during the work with PBA, stated in protocols of the commission for monitoring compliance with biological safety requirements of the Volgograd Research Anti-Plague Institute over the period of 1986β2020. Assessed were the type of emergency, their number, main causes and prerequisites for occurrence, professional category of a worker who participated in an accident.Results and discussion. During the specified period 3 types of accidents were recorded: accidents with spraying, accidents with skin lesion, accidents without spraying. There were no accidents with damage to the insulating suit and the pneumatic suit during the entire period under investigation. Of the total number of accidents, 42.85 % of cases were associated with skin lesion due to the bite of an experimental animal due to its incorrect fixation during infection, feeding, care, or due to autopsy of animals. Spillage accidents were recorded in 42.85 %; accidents without spraying amounted to 14.2 %. The categories of employees who made the greatest number of accidents have been identified: laboratory assistants β 39.2 % of cases, researcher officers β 14.2 %, disinfectors β 14.2 %. The causes of accidents and the prerequisites contributing to their realization have been pinpointed. The main ways and measures to reduce the risks of emergency situations for personnel when working with pathogens of particularly dangerous infectious diseases are put forward
Study of Protective Properties of Gentamicin in Liposome-Entrapped and Free Forms in Case of Aerogenic Melioidosis Infection
Presented are data on investigation of protective properties of free and liposome-entrapped forms of gentamicin against aerogenic infection caused by melioidosis agent. Comparative study identified that immobilization of gentamicin into liposomes increased substantially the ED50 of the preparation. This, probably, can be related to higher sorbtion of liposomal preparation by parenchymal organs including lungs
EMERGENCY PROPHYLAXIS OF EXPERIMENTAL MELIOIDOSIS USING SYNTHETIC IMMUNOMODULATORS AND HETEROLOGOUS VACCINES
Melioidosis is a particularly dangerous infection caused by Burkholderia pseudomallei , against which a vaccine has not yet been created. In this regard, the development of effective treatment regimens and emergency prevention of melioidosis is very relevant. To improve the effectiveness of emergency prophylaxis of melioidosis, synthetic peptides (bestim, imunofan) and thiopoietin preparation glutoxim were used when combined with the antibiotic doxycycline. In addition, in experiments on white mice, the ability of heterologous vaccines (plague and tularemia), used in the emergency prevention mode, to increase the resistance of animals to melioidosis infection was assessed. It was shown that the most effective was imunofan, which, when combined with doxycycline, increased by 20% the survival rate of 5LD50 Burkholderia pseudomallei infection and significantly increased the average life span of mice infected with 5-12 LD50 (p < 0.05). The efficiency of use for stimulation of non-specific resistance to melioidosis of a heterologous plague vaccine EV, once administered 1 day prior to infection, protected 90% of mice from 6 LD50 Burkholderia pseudomallei and 60% - with an increase in the infectious dose of the pathogen of melioidosis up to 15 LD50. The same level of protection from melioidosis was provided by a 3 day course of antibiotic therapy with doxycycline. It was concluded that the tularemia vaccine is not suitable for immunostimulation in melioidosis due to its high residual virulence and reactogenicity
Application of Cytokines and Synthetic Peptides for Increase in Immunogenicity of Melioidosis Antigens
B. pseudomallei 100 resistance (61.5 % of survived animals). It was concluded that it is a good practice to include bestim in the schedule of complex immunization against melioidosis
ΠΠ‘ΠΠΠΠΠΠΠ‘Π’Π ΠΠΠΠ€ΠΠΠΠΠΠ¦ΠΠ ΠΠΠΠΠΠΠΠΠ‘ΠΠΠΠΠ¬ΠΠΠΠ ΠΠ ΠΠΠΠ ΠΠ’Π
The conditions of lyophilization of nanoliposomes with encapsulated gentamicin (liposomal gentamicin) were studied. A mode of its safe lyophilization was suggested. A program for eliminating adverse factors was developed. It includes smooth increase of temperature from -70Β°C (freezing temperature) to room temperature (22Β±2)Β°C and gradual reduction of vacuum from 30 to 10 Pa without jumps and drops. The characteristics of the labile nanoliposomal drugs were not lost. The suggested program consisted of 10 steps allowing computer control to attain a slow and uniform increase in the temperature of the frozen product. The precise control of pressure in the chamber was provided by a system of automatic regulation of vacuum supplied with the equipment. After the rehydration the lyophilized preparations completely restored their original shape and properties.ΠΠ·ΡΡΠ΅Π½Ρ ΡΡΠ»ΠΎΠ²ΠΈΡ Π»ΠΈΠΎΡΠΈΠ»ΠΈΠ·Π°ΡΠΈΠΈ Π½Π°Π½ΠΎΠ»ΠΈΠΏΠΎΡΠΎΠΌ Ρ ΠΈΠ½ΠΊΠ°ΠΏΡΡΠ»ΠΈΡΠΎΠ²Π°Π½Π½ΡΠΌ Π³Π΅Π½ΡΠ°ΠΌΠΈΡΠΈΠ½ΠΎΠΌ (Π»ΠΈΠΏΠΎΡΠΎΠΌΠ°Π»ΡΠ½ΡΠΉ Π³Π΅Π½ΡΠ°ΠΌΠΈΡΠΈΠ½). ΠΡΠ΅Π΄Π»ΠΎΠΆΠ΅Π½ ΠΌΡΠ³ΠΊΠΈΠΉ ΡΠ΅Ρ
Π½ΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΠΉ ΡΠ΅ΠΆΠΈΠΌ Π»ΠΈΠΎΡΠΈΠ»ΠΈΠ·Π°ΡΠΈΠΈ ΠΏΠΎ ΡΠ°Π·ΡΠ°Π±ΠΎΡΠ°Π½Π½ΠΎΠΉ ΠΏΡΠΎΠ³ΡΠ°ΠΌΠΌΠ΅ Π°Π²ΡΠΎΠΌΠ°ΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΡΠΏΡΠ°Π²Π»Π΅Π½ΠΈΡ ΠΏΠ°ΡΠ°ΠΌΠ΅ΡΡΠ°ΠΌΠΈ. ΠΡΠ΅Π΄Π»Π°Π³Π°Π΅ΠΌΠ°Ρ ΠΏΡΠΎΠ³ΡΠ°ΠΌΠΌΠ° ΡΠΎΡΡΠΎΠΈΡ ΠΈΠ· 10 ΡΠ°Π³ΠΎΠ² Π΄Π»Ρ Π΄ΠΎΡΡΠΈΠΆΠ΅Π½ΠΈΡ ΠΌΠ΅Π΄Π»Π΅Π½Π½ΠΎΠ³ΠΎ ΠΈ ΡΠ°Π²Π½ΠΎΠΌΠ΅ΡΠ½ΠΎΠ³ΠΎ ΠΏΠΎΠ²ΡΡΠ΅Π½ΠΈΡ ΡΠ΅ΠΌΠΏΠ΅ΡΠ°ΡΡΡΡ Π·Π°ΠΌΠΎΡΠΎΠΆΠ΅Π½Π½ΠΎΠ³ΠΎ ΠΏΡΠΎΠ΄ΡΠΊΡΠ°. ΠΠ»Ρ ΡΡΡΡΠ°Π½Π΅Π½ΠΈΡ Π½Π΅Π±Π»Π°Π³ΠΎΠΏΡΠΈΡΡΠ½ΡΡ
ΡΠ°ΠΊΡΠΎΡΠΎΠ² Π² ΠΏΡΠΎΠ³ΡΠ°ΠΌΠΌΠ΅, ΠΏΡΠ΅Π΄ΡΡΠΌΠΎΡΡΠ΅Π½ΠΎ ΠΏΠ»Π°Π²Π½ΠΎΠ΅ ΡΠ²Π΅Π»ΠΈΡΠ΅Π½ΠΈΠ΅ ΡΠ΅ΠΌΠΏΠ΅ΡΠ°ΡΡΡΡ ΠΎΡ -70Β°Π‘ (ΡΠ΅ΠΌΠΏΠ΅ΡΠ°ΡΡΡΠ° Π·Π°ΠΌΠΎΡΠ°ΠΆΠΈΠ²Π°Π½ΠΈΡ) Π΄ΠΎ ΠΊΠΎΠΌΠ½Π°ΡΠ½ΠΎΠΉ ΡΠ΅ΠΌΠΏΠ΅ΡΠ°ΡΡΡΡ (22Β±2)Β°Π‘ ΠΈ ΠΏΠΎΡΡΠ΅ΠΏΠ΅Π½Π½ΠΎΠ΅ ΡΠ½ΠΈΠΆΠ΅Π½ΠΈΠ΅ Π²Π°ΠΊΡΡΠΌΠ° ΠΎΡ 30 Π΄ΠΎ 10 ΠΠ° Π±Π΅Π· ΡΠΊΠ°ΡΠΊΠΎΠ² ΠΈ ΠΏΠ΅ΡΠ΅ΠΏΠ°Π΄ΠΎΠ². Π’ΠΎΡΠ½ΡΠΉ ΠΊΠΎΠ½ΡΡΠΎΠ»Ρ Π΄Π°Π²Π»Π΅Π½ΠΈΡ Π² ΠΊΠ°ΠΌΠ΅ΡΠ΅ ΠΎΠ±Π΅ΡΠΏΠ΅ΡΠΈΠ²Π°Π΅ΡΡΡ ΡΠΈΡΡΠ΅ΠΌΠΎΠΉ Π°Π²ΡΠΎΠΌΠ°ΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΡΠ΅Π³ΡΠ»ΠΈΡΠΎΠ²Π°Π½ΠΈΡ Π²Π°ΠΊΡΡΠΌΠ°, Π²Ρ
ΠΎΠ΄ΡΡΠ΅Π³ΠΎ Π² ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡ ΠΏΠΎΡΡΠ°Π²ΠΊΠΈ ΠΎΠ±ΠΎΡΡΠ΄ΠΎΠ²Π°Π½ΠΈΡ. ΠΠΎΡΠ»Π΅ ΡΠ΅Π³ΠΈΠ΄ΡΠ°ΡΠ°ΡΠΈΠΈ Π»ΠΈΠΎΡΠΈΠ»ΠΈΠ·ΠΈΡΠΎΠ²Π°Π½Π½ΡΠ΅ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΡ ΠΏΠΎΠ»Π½ΠΎΡΡΡΡ Π²ΠΎΡΡΡΠ°Π½ΠΎΠ²ΠΈΠ»ΠΈ ΡΠ²ΠΎΡ ΠΏΠ΅ΡΠ²ΠΎΠ½Π°ΡΠ°Π»ΡΠ½ΡΡ ΡΠΎΡΠΌΡ ΠΈ ΡΠ²ΠΎΠΉΡΡΠ²Π°
Peculiarities of Epidemiological Situation on the West Nile Fever in 2012 in the Territory of the Russian Federation
During the epidemic season of 2012 453 cases of West Nile fever (WNF) were registered in the territory of the 21 constituent entities of the Russian Federation. Epidemic process is spreading itself towards the northern regions of Russia. The presence of markers of West Nile fever virus in carriers and vectors of the disease as well as acquired immunity among the population against the West Nile virus (WNV) have been identified in the territory of 53 constituent entities of the Russian Federation. The epidemic process is characterized by a number of peculiarities concerning both its epidemiological and clinical aspects. With the intensification of diagnostic works among the patients with clinical symptomatology similar to WNF, first and foremost in the territories where WNV markers have been detected, prognosticated is the registration of WNF incidence among the population extending over a large area of the Russian Federation
Features of lyophilization of a nanoliposomal drug
The conditions of lyophilization of nanoliposomes with encapsulated gentamicin (liposomal gentamicin) were studied. A mode of its safe lyophilization was suggested. A program for eliminating adverse factors was developed. It includes smooth increase of temperature from -70Β°C (freezing temperature) to room temperature (22Β±2)Β°C and gradual reduction of vacuum from 30 to 10 Pa without jumps and drops. The characteristics of the labile nanoliposomal drugs were not lost. The suggested program consisted of 10 steps allowing computer control to attain a slow and uniform increase in the temperature of the frozen product. The precise control of pressure in the chamber was provided by a system of automatic regulation of vacuum supplied with the equipment. After the rehydration the lyophilized preparations completely restored their original shape and properties