The RNA chaperon activity of the human La protein (LARP3): DOI: 10.14800/rd.872

Single-stranded RNA molecules fold intensively into secondary and tertiary structures and are often trapped in non-functional configurations. To adapt a functional configuration, structural changes have to be achieved. RNA helicases and RNA chaperones are proteins able to assist those structural rearrangements in an ATP-dependent or ATP-independent manner, respectively. The cancer-associated RNA-binding protein La (LARP3) is an RNA chaperone involved in various aspects of the RNA metabolism. Recently the RNA chaperone domain within the human La protein has been mapped and demonstrated that its activity is required to stimulate cyclin D1-internal ribosome entry site (IRES)-dependent protein synthesis. Furthermore, it has been shown that the La protein can be phosphorylated by serine/threonine kinase AKT in vitro. Taken together, we suggest a model in which the RNA chaperone La stimulates translation of specific target mRNAs by assisting structural changes in their translation start site surrounding RNA region

Results of experimental studies of geologic profile over hydrocarbon deposits

The article presents the results of experimental studies of geologic profile over hydrocarbon deposits in the Republic of Belarus. Experimental studies were carried out at the Marmovichi and the Geologicheskoye fields of hydrocarbon deposits in the Gomel region. Measurement of the phase of the surface impedance of the geological profile over hydrocarbon deposits under the influence of electromagnetic waves with amplitude-frequency modulation was carried out. It has been found that the use of two modes for measuring the phase of surface impedance increases the accuracy of delineation of hydrocarbon deposits. A method at carrier frequencies of 0.1...2 GHz for amplitude-frequency modulated signals has been developed. The above studies can be applied to the development of methods for exploration of hydrocarbon deposits

Mapping of UK Biobank clinical codes: Challenges and possible solutions.

ObjectiveThe UK Biobank provides a rich collection of longitudinal clinical data coming from different healthcare providers and sources in England, Wales, and Scotland. Although extremely valuable and available to a wide research community, the heterogeneous dataset contains inconsistent medical terminology that is either aligned to several ontologies within the same category or unprocessed. To make these data useful to a research community, data cleaning, curation, and standardization are needed. Significant efforts to perform data reformatting, mapping to any selected ontologies (such as SNOMED-CT) and harmonization are required from any data user to integrate UK Biobank hospital inpatient and self-reported data, data from various registers with primary care (GP) data. The integrated clinical data would provide a more comprehensive picture of one's medical history.Materials and methodsWe evaluated several approaches to map GP clinical Read codes to International Classification of Diseases (ICD) and Systematized Nomenclature of Medicine Clinical Terms (SNOMED CT) terminologies. The results were compared, mapping inconsistencies were flagged, a quality category was assigned to each mapping to evaluate overall mapping quality.ResultsWe propose a curation and data integration pipeline for harmonizing diagnosis. We also report challenges identified in mapping Read codes from UK Biobank GP tables to ICD and SNOMED CT.Discussion and conclusionSome of the challenges-the lack of precise one-to-one mapping between ontologies or the need for additional ontology to fully map terms-are general reflecting trade-offs to be made at different steps. Other challenges are due to automatic mapping and can be overcome by leveraging existing mappings, supplemented with automated and manual curation