18 research outputs found
Decellularised skeletal muscles allow functional muscle regeneration by promoting host cell migration
Pathological conditions affecting skeletal muscle function may lead to irreversible volumetric
muscle loss (VML). Therapeutic approaches involving acellular matrices represent an
emerging and promising strategy to promote regeneration of skeletal muscle following injury.
Here we investigated the ability of three different decellularised skeletal muscle scaffolds to
support muscle regeneration in a xenogeneic immune-competent model of VML, in which
the EDL muscle was surgically resected. All implanted acellular matrices, used to replace
the resected muscles, were able to generate functional artificial muscles by promoting host
myogenic cell migration and differentiation, as well as nervous fibres, vascular networks, and
satellite cell (SC) homing. However, acellular tissue mainly composed of extracellular matrix
(ECM) allowed better myofibre three-dimensional (3D) organization and the restoration of
SC pool, when compared to scaffolds which also preserved muscular cytoskeletal
structures. Finally, we showed that fibroblasts are indispensable to promote efficient
migration and myogenesis by muscle stem cells across the scaffolds in vitro. This data strongly support the use of xenogeneic acellular muscles as device to treat VML conditions in absence of donor cell implementation, as well as in vitro model for studying cell interplay during myogenesis
Inhibiting adenosine deaminase modulates the systemic inflammatory response syndrome in endotoxemia and sepsis
A drug-laden elastomer for surgical treatment of anal fistula
10.1007/s13346-011-0044-0Drug Delivery and Translational Research16439-44
Long-term quality of life after endoscopic dilation of strictured colorectal or colocolonic anastomoses
Rehabilitative exercise and spatially patterned nanofibrillar scaffolds enhance vascularization and innervation following volumetric muscle loss
NIR Spectroscopy Applications in the Development of a Compacted Multiparticulate System for Modified Release
The purpose of this study was to utilize near-infrared spectroscopy and chemical imaging to characterize extrusion-spheronized drug beads, lipid-based placebo beads, and modified release tablets prepared from blends of these beads. The tablet drug load (10.5–19.5 mg) of theophylline (2.25 mg increments) and cimetidine (3 mg increments) could easily be differentiated using univariate analyses. To evaluate other tablet attributes (i.e., compression force, crushing force, content uniformity), multivariate analyses were used. Partial least squares (PLS) models were used for prediction and principal component analysis (PCA) was used for classification. The PLS prediction models (R2 > 0.98) for content uniformity of uncoated compacted theophylline and cimetidine beads produced the most robust models. Content uniformity data for tablets with drug content ranging between 10.5 and 19.5 mg showed standard error of calibration (SEC), standard error of cross-validation, and standard error of prediction (SEP) values as 0.31, 0.43, and 0.37 mg, and 0.47, 0.59, and 0.49 mg, for theophylline and cimetidine, respectively, with SEP/SEC ratios less than 1.3. PCA could detect blend segregation during tableting for preparations using different ratios of uncoated cimetidine beads to placebo beads (20:80, 50:50, and 80:20). Using NIR chemical imaging, the 80:20 formulations showed the most pronounced blend segregation during the tableting process. Furthermore, imaging was capable of quantitating the cimetidine bead content among the different blend ratios. Segregation testing (ASTM D6940-04 method) indicated that blends of coated cimetidine beads and placebo beads (50:50 ratio) also tended to segregate