Reposicionamento horizontal mandibular com próteses provisórias removíveis tipo overlay – relato de caso Mandibular horizontal repositioning with removable temporary overlay prostheses – case report

Introduction: Rehabilitation with the use of overlay-type removable prostheses is indicated in cases of more severe occlusal dysfunction. This type of treatment meets the need for more immediate results, providing aesthetic improvement while the rehabilitation work is being done, while promoting the gradual rehabilitation of occlusal function, re-adjusting the intermaxillary relationship. In addition, the patient can anticipate the final result of the treatment beforethe definitive prosthesis is made. Objective: To show a clinical case of planning and execution of removable overlays. Case report: JCF patient had occlusal dysfunction and presented as main complaint the aesthetic and functional deficiency. He used a removable partial denture (PPR) only on the upper arch. The existing prosthesis did not present stability due to the loss of some dental elements, pillars of the PPR. Two overlays (upper and lower) were indicated because the patient had good periodontal conditions in the remaining dentalelements that were in a favorable position. Necessary exodontia, adequacy of the buccal environment with sub and supragingival scaling and the fabrication of overlays were performed. Conclusion: The use of temporary overlays can provide the necessary time for the patient to adapt to the new occlusal conformation in the period in which the restorations and definitive prostheses are expected to be performed. The use of overlays presented a satisfactoryresult for the proposed treatment, since it promoted the functional rehabilitation, restored aesthetic, even temporarily, and motivated the patient to continue the treatment. According to the patient, there were significant improvements in chewing quality and self-esteem.Introduction: Rehabilitation with the use of overlay-type removable prostheses is indicated in cases of more severe occlusal dysfunction. This type of treatment meets the need for more immediate results, providing aesthetic improvement while the rehabilitation work is being done, while promoting the gradual rehabilitation of occlusal function, re-adjusting the intermaxillary relationship. In addition, the patient can anticipate the final result of the treatment beforethe definitive prosthesis is made. Objective: To show a clinical case of planning and execution of removable overlays. Case report: JCF patient had occlusal dysfunction and presented as main complaint the aesthetic and functional deficiency. He used a removable partial denture (PPR) only on the upper arch. The existing prosthesis did not present stability due to the loss of some dental elements, pillars of the PPR. Two overlays (upper and lower) were indicated because the patient had good periodontal conditions in the remaining dentalelements that were in a favorable position. Necessary exodontia, adequacy of the buccal environment with sub and supragingival scaling and the fabrication of overlays were performed. Conclusion: The use of temporary overlays can provide the necessary time for the patient to adapt to the new occlusal conformation in the period in which the restorations and definitive prostheses are expected to be performed. The use of overlays presented a satisfactoryresult for the proposed treatment, since it promoted the functional rehabilitation, restored aesthetic, even temporarily, and motivated the patient to continue the treatment. According to the patient, there were significant improvements in chewing quality and self-esteem

Effect of hydrogen peroxide application on color and surface roughness of two restorative materials

is study investigated the effect of an in-office bleaching technique on lightness, color and surface roughness of two commercially available materials: a resin-modified glass-ionomer cement and a nanohybrid resin composite. Methods: Twelve disk-shaped specimens were prepared with both materials. The samples were bleached with 35% hydrogen peroxide. Bleaching was tested initially onto a smooth surface and later onto a polished one of the same specimens. The effect of the treatments on lightness and color was verified with a spectrophotometer. Surface roughness was measured with a digital surface roughness tester. The data were statistically analyzed by repeated measures ANOVA and post hoc Tukeys test (alpha = 0.05). Results: Significant variation in lightness and color was observed on the resin-modified glass-ionomer cement after the first bleaching procedure. Roughness increased significantly only after polishing the resin-modified glass-ionomer cement surface. Composite color variation was evident in the last observation period, but roughness and lightness variation due to bleaching and polishing was not significant. Conclusion: The bleaching treatment caused significant color alterations on the materials tested. This study observed that the application of in-office bleaching onto the glass-ionomer cement promoted clinically observable color alteration, and polishing after bleaching is contraindicated for this material

Natural Membrane Differentiates Human Adipose-Derived Mesenchymal Stem Cells to Neurospheres by Mechanotransduction Related to YAP and AMOT Proteins

Adipose tissue-derived mesenchymal stem cells (ADMSCs) are promising candidates for regenerative medicine, as they have good cell yield and can differentiate into several cell lines. When induced to the neuronal differentiation, they form neurospheres composed of neural precursors (NPs) that can be an alternative in treating neurodegenerative diseases. This study aimed to characterize NPs from neurospheres obtained after seeding ADMSCs on a natural polyisoprene-based membrane. The ADMSCs were isolated from adipose tissue by enzymatic dissociation, were subjected to trilineage differentiation, and were characterized by flow cytometry for specific ADMSC surface markers. For neuronal differentiation, the cells were seeded on polystyrene flasks coated with the membrane and were characterized by immunocytochemistry and RT-PCR. The results demonstrated that the isolated cells showed characteristics of ADMSCs. At 15 to 25 days, ADMSCs seeded on the natural membrane developed neurospheres. Then, after dissociation, the cells demonstrated characteristic neuronal markers expressed on NPs: nestin, ß-III tubulin, GFAP, NeuN, and the YAP1/AMOT in the cytoplasm. In conclusion, it was demonstrated that this membrane differentiates the ADMSCs to NPs without any induction factors, and suggests that their differentiation mechanisms are related to mechanotransduction regulated by the YAP and AMOT proteins

Laser Ablated Albumin Functionalized Spherical Gold Nanoparticles Indicated for Stem Cell Tracking

Cell tracking in cell-based therapy applications helps distinguish cell participation among paracrine effect, neovascularization, and matrix deposition. This preliminary study examined the cellular uptake of gold nanoparticles (AuNPs), observing cytotoxicity and uptake of different sizes and AuNPs concentrations in Adipose-derived stromal cells (ASCs). ASCs were incubated for 24 h with Laser ablated Albumin functionalized spherical AuNPs (LA-AuNPs), with average sizes of 2 nm and 53 nm in diameter, in four concentrations, 127 µM, 84 µM, 42 µM, and 23 µM. Cytotoxicity was examined by Live/Dead assay, and erythrocyte hemolysis, and the effect on the cytoskeleton was investigated by immunocytochemistry for β-actin. The LA-AuNPs were internalized by the ASCs in a size and concentration-dependent manner. Clusters were observed as dispersed small ones in the cytosol, and as a sizeable perinuclear cluster, without significant harmful effects on the cells for up to 2 weeks. The Live/Dead and hemolysis percentage results complemented the observations that the larger 53 nm LA-AuNPs in the highest concentrated solution significantly lowered cell viability. The demonstrated safety, cellular uptake, and labelling persistency with LA-AuNPs, synthesized without the combination of chemical solutions, support their use for cell tracking in tissue engineering applications

Adipose-Derived Stromal Cells and Mineralized Extracellular Matrix Delivery by a Human Decellularized Amniotic Membrane in Periodontal Tissue Engineering

Periodontitis is a prevalent disease characterized by the loss of periodontal supporting tissues, bone, periodontal ligament, and cementum. The application of a bone tissue engineering strategy with Decellularized Human Amniotic Membrane (DAM) with adipose-derived stromal cells (ASCs) has shown to be convenient and valuable. This study aims to investigate the treatments of a rat periodontal furcation defect model with DAM, ASCs, and a mineralized extracellular matrix (ECM). Rat ASCs were expanded, cultivated on DAM, and with a bone differentiation medium for four weeks, deposited ECM on DAM. Periodontal healing for four weeks was evaluated by micro-computed tomography and histological analysis after treatments with DAM, ASCs, and ECM and compared to untreated defects on five consecutive horizontal levels, from gingival to apical. The results demonstrate that DAM preserves its structure during cultivation and healing periods, supporting cell attachment, permeation, bone deposition on DAM, and periodontal regeneration. DAM and DAM+ASCs enhance bone healing compared to the control on the gingival level. In conclusion, DAM with ASC or without cells and the ECM ensures bone tissue healing. The membrane supported neovascularization and promoted osteoconduction

The Neural Multilineage Differentiation Capacity of Human Neural Precursors from the Umbilical Cord—Ready to Bench for Clinical Trials

Mesenchymal stem cells (MSC) are promising for regenerative medicine as they have a vast differentiation capacity, immunomodulatory properties and can be isolated from different tissues. Among them, the umbilical cord is considered a good source of MSC, as its collection poses no risk to donors and is unrelated to ethical issues. Furthermore, umbilical cord mesenchymal stem cells (UC-MSC) can differentiate into several cell lines, including neural lineages that, in the future, may become an alternative in the treatment of neurodegenerative diseases. This study used a natural functional biopolymer matrix (NFBX) as a membrane to differentiate UC-MSC into neurospheres and their Neural precursors without using neurogenic growth factors or gene transfection. Through the characterization of Neural precursors and differentiated cells, it was possible to demonstrate the broad potential for the differentiation of cells obtained through cultivation on this membrane. To demonstrate these Neural precursors’ potential for future studies in neurodegenerative diseases, the Neural precursors from Wharton’s jelly were differentiated into Schwann cells, oligodendrocytes, cholinergic-, dopaminergic- and GABAergic-like neurons

Demyelination Lesions Do Not Correlate with Clinical Manifestations by Bordetella pertussis Toxin Concentrations

Multiple sclerosis (MS) is an autoimmune disease of the central nervous system, characterized as an inflammatory demyelinating disease. Given the need for improvements in MS treatment, many studies are mainly conducted through preclinical models such as experimental allergic encephalomyelitis (EAE). This study analyzes the relationships between histopathological and clinical score findings at EAE. Twenty-three female Rattus norvegicus Lewis rats from 6 to 8 weeks were induced to EAE. Nineteen rats underwent EAE induction distributed in six groups to establish the evolution of clinical signs, and four animals were in the control group. Bordetella pertussis toxin (PTX) doses were 200, 250, 300, 350 and 400 ng. The clinical scores of the animals were analyzed daily, from seven to 24 days after induction. The brains and spinal cords were collected for histopathological analyses. The results demonstrated that the dose of 250 ng of PTX induced a higher clinical score and reduction in weight. All induced groups demonstrated leukocyte infiltration, activation of microglia and astrocytes, and demyelinated plaques in the brains in histopathology. It was concluded that the dose of 250 ng and 350 ng of PTX were the best choices to trigger the brain and spinal cord demyelination lesions and did not correlate with clinical scores

State of the Art of microRNAs Signatures as Biomarkers and Therapeutic Targets in Parkinson’s and Alzheimer’s Diseases: A Systematic Review and Meta-Analysis

Identifying target microRNAs (miRNAs) might serve as a basis for developing advanced therapies for Parkinson’s disease (PD) and Alzheimer’s disease. This review aims to identify the main therapeutic targets of miRNAs that can potentially act in Parkinson’s and Alzheimer’s diseases. The publication research was conducted from May 2021 to March 2022, selected from Scopus, PubMed, Embase, OVID, Science Direct, LILACS, and EBSCO. A total of 25 studies were selected from 1549 studies evaluated. The total number of miRNAs as therapeutic targets evidenced was 90 for AD and 54 for PD. An average detection accuracy of above 84% for the miRNAs was observed in the selected studies of AD and PD. The major signatures were miR-26b-5p, miR-615-3p, miR-4722-5p, miR23a-3p, and miR-27b-3p for AD and miR-374a-5p for PD. Six miRNAs of intersection were found between AD and PD. This article identified the main microRNAs as selective biomarkers for diagnosing PD and AD and therapeutic targets through a systematic review and meta-analysis. This article can act as a microRNA guideline for laboratory research and pharmaceutical industries for treating Alzheimer’s and Parkinson’s diseases and offers the opportunity to evaluate therapeutic interventions earlier in the disease process

Biodegradable Nanoparticles Loaded with Levodopa and Curcumin for Treatment of Parkinson’s Disease

International audienceBackground: Parkinson’s disease (PD) is the second most common age-related neurodegenerative disorder. Levodopa (L-DOPA) remains the gold-standard drug available for treating PD. Curcumin has many pharmacological activities, including antioxidant, anti-inflammatory, antimicrobial, anti-amyloid, and antitumor properties. Copolymers composed of Poly (ethylene oxide) (PEO) and biodegradable polyesters such as Poly (ε-caprolactone) (PCL) can self-assemble into nanoparticles (NPs). This study describes the development of NH2–PEO–PCL diblock copolymer positively charged and modified by adding glutathione (GSH) on the outer surface, resulting in a synergistic delivery of L-DOPA curcumin that would be able to pass the blood–brain barrier. Methods: The NH2–PEO–PCL NPs suspensions were prepared by using a nanoprecipitation and solvent displacement method and coated with GSH. NPs were submitted to characterization assays. In order to ensure the bioavailability, Vero and PC12 cells were treated with various concentrations of the loaded and unloaded NPs to observe cytotoxicity. Results: NPs have successfully loaded L-DOPA and curcumin and were stable after freeze-drying, indicating advancing into in vitro toxicity testing. Vero and PC12 cells that were treated up to 72 h with various concentrations of L-DOPA and curcumin-loaded NP maintained high viability percentage, indicating that the NPs are biocompatible. Conclusions: NPs consisting of NH2–PEO–PCL were characterized as potential formulations for brain delivery of L-DOPA and curcumin. The results also indicate that the developed biodegradable nanomicelles that were blood compatible presented low cytotoxicity

Fluorescence properties of curcumin-loaded nanoparticles for cell tracking

Background: Posttransplant cell tracking, via stem cell labeling, is a crucial strategy for monitoring and maximizing benefits of cell-based therapies. The structures and functionalities of polysaccharides, proteins, and lipids allow their utilization in nanotechnology systems. Materials and methods: In the present study, we analyzed the potential benefit of curcumin-loaded nanoparticles (NPC) using Vero cells (in vitro) and NPC-labeled adipose-derived mesenchymal stem cells (NPC-ADMSCs) (in vivo) in myocardial infarction and sciatic nerve crush preclinical models. Thereafter, transplantation, histological examination, real time imaging, and assessment of tissue regeneration were done. Results: Transplanted NPC-ADMSCs were clearly identified and revealed potential benefit when used in cell tracking. Conclusion: This approach may have broad applications in modeling labeled transplanted cells and in developing improved stem cell therapeutic strategies.Fil: Mogharbel, Bassam Felipe. Pelé Pequeno Príncipe Institute; BrasilFil: Francisco, Julio Cesar. Pelé Pequeno Príncipe Institute; BrasilFil: Irioda, Ana Carolina. Pelé Pequeno Príncipe Institute; BrasilFil: Dziedzic, Dilcele Silva Moreira. Pelé Pequeno Príncipe Institute; BrasilFil: Ferreira, Priscila Elias. Pelé Pequeno Príncipe Institute; BrasilFil: de Souza, Daiany. Pelé Pequeno Príncipe Institute; BrasilFil: de Souza, Carolina Maria Costa Oliveira. Pelé Pequeno Príncipe Institute; BrasilFil: Neto, Nelson Bergonse. Pelé Pequeno Príncipe Institute; BrasilFil: Guarita Souza, Luiz Cesar. Universidade Federal do Paraná; BrasilFil: Franco, Celia Regina Cavichiolo. Universidade Federal do Paraná; BrasilFil: Nakamura, Celso Vataru. Universidade Estadual de Maringá; BrasilFil: Kaplum, Vanessa. Universidade Estadual de Maringá; BrasilFil: Mazzarino, Letícia. Universidade Federal de Santa Catarina; BrasilFil: Lemos Senna, Elenara. Universidade Federal de Santa Catarina; BrasilFil: Borsali, Redouan. Universite Grenoble Alpes; FranciaFil: Soto, Paula Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaFil: Setton, Clara Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaFil: Abdelwahid, Eltyeb. Northwestern University; Estados UnidosFil: Athayde Teixeira de Carvalho, Katherine. Pelé Pequeno Príncipe Institute; Brasi