Near-source passive sampling for monitoring viral outbreaks within a university residential setting

\ua9 2024 Cambridge University Press. All rights reserved. Wastewater based epidemiology (WBE) has proven to be a powerful tool for the population-level monitoring of pathogens, particularly SARS-CoV-2. For accurate and timely assessment, several wastewater sampling regimes and methods of viral concentration have been investigated, mainly targeting SARS-CoV-2. However, the use of passive samplers in near-source environments for a range of viruses in wastewater is yet under-investigated. To address this, near-source passive samples were taken at four locations targeting student halls of residence. These were chosen as an exemplar due to their high population density and perceived risk of disease transmission. Viruses investigated were SARS-CoV-2 and its variants of concern (VOCs), influenza-A and B viruses and enteroviruses. Sampling was conducted either in the morning, where passive samplers were in place overnight (17 h) and during the day, where samplers remained in the sewer for 7 h. We demonstrated the usefulness of near-source passive sampling for the detection of VOCs using qPCR and Next Generation Sequencing. Furthermore, several outbreaks of influenza-A and sporadic outbreaks of enteroviruses (some associated with enterovirus D68 and coxsackieviruses) were identified amongst the resident student population, providing evidence of the usefulness of near-source, in-sewer sampling for monitoring the health of high population density communities