5 research outputs found
ΠΠΠΠΠΠ ΠΠΠ― ΠΠΠΠ ΠΠΠΠΠ’Π Π ΠΠΠΠΠΠΠΠΠΠΠ― ΠΠΠΠ§ΠΠ«Π₯ ΠΠ£Π’ΠΠ
Traditionally, the biliary tract has been considered to be normally sterile, and the presence of microorganisms in bile is a marker of a pathological process. This assumption was confirmed by failure in allocation of bacterial strains from the normal bile duct. The paper provides rationale for a phenomenon of the normal biliary microbiota as a separate functional layer which protects a biliary tract from colonization by exogenous microorganisms. We revealed the potential of metagenomic data for prevention of infectious diseases, post-operative complications of reconstructive interventions including bile duct stenting and implantation the tissue-engineered structures exposed to the risks of colonization with pathogenic / exogenous microorganisms. The methods based on preserving homeostasis of normal biliary microbiota ecosystem can be used for prevention of hepatobiliary diseases and treatment of biliary tract inflammatory diseases.Π’ΡΠ°Π΄ΠΈΡΠΈΠΎΠ½Π½ΠΎ ΡΡΠΈΡΠ°Π»ΠΎΡΡ, ΡΡΠΎ ΠΆΠ΅Π»ΡΠ½ΡΠΉ ΠΏΡΠΎΡΠΎΠΊ ΡΡΠ΅ΡΠΈΠ»Π΅Π½, Π° ΠΏΡΠΈΡΡΡΡΡΠ²ΠΈΠ΅ Π² ΠΆΠ΅Π»ΡΠΈ ΠΌΠΈΠΊΡΠΎΠΎΡΠ³Π°Π½ΠΈΠ·ΠΌΠΎΠ² ΡΠ²Π»ΡΠ΅ΡΡΡ ΠΌΠ°ΡΠΊΠ΅ΡΠΎΠΌ ΠΏΠ°ΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΏΡΠΎΡΠ΅ΡΡΠ°. ΠΠΎΠ΄ΠΎΠ±Π½ΠΎΠ΅ ΠΏΡΠ΅Π΄ΠΏΠΎΠ»ΠΎΠΆΠ΅Π½ΠΈΠ΅ ΠΏΠΎΠ΄ΡΠ²Π΅ΡΠΆΠ΄Π°Π»ΠΎΡΡ Π±Π΅Π·ΡΡΠΏΠ΅ΡΠ½ΠΎΡΡΡΡ Π²ΡΠ΄Π΅Π»Π΅Π½ΠΈΡ Π±Π°ΠΊΡΠ΅ΡΠΈΠ°Π»ΡΠ½ΡΡ
ΡΡΠ°ΠΌΠΌΠΎΠ² ΠΈΠ· Π½ΠΎΡΠΌΠ°Π»ΡΠ½ΠΎΠ³ΠΎ ΠΆΠ΅Π»ΡΠ½ΠΎΠ³ΠΎ ΠΏΡΠΎΡΠΎΠΊΠ°. Π Π½Π°ΡΡΠΎΡΡΠ΅ΠΉ ΡΠ°Π±ΠΎΡΠ΅ ΠΏΡΠΈΠ²ΠΎΠ΄ΠΈΡΡΡ ΠΎΠ±ΠΎΡΠ½ΠΎΠ²Π°Π½ΠΈΠ΅ ΡΠ΅Π½ΠΎΠΌΠ΅Π½Π° Π½ΠΎΡΠΌΠ°Π»ΡΠ½ΠΎΠΉ ΠΌΠΈΠΊΡΠΎΠ±ΠΈΠΎΡΡ ΠΆΠ΅Π»ΡΠ΅Π²ΡΠ²ΠΎΠ΄ΡΡΠΈΡ
ΠΏΡΡΠ΅ΠΉ ΠΊΠ°ΠΊ ΠΎΡΠ΄Π΅Π»ΡΠ½ΠΎΠ³ΠΎ ΡΠ»ΠΎΡ, ΠΊΠΎΡΠΎΡΡΠΉ Π·Π°ΡΠΈΡΠ°Π΅Ρ ΠΆΠ΅Π»ΡΠ½ΡΠ΅ ΠΏΡΡΠΈ ΠΎΡ ΠΊΠΎΠ»ΠΎΠ½ΠΈΠ·Π°ΡΠΈΠΈ ΡΠΊΠ·ΠΎΠ³Π΅Π½Π½ΡΠΌΠΈ ΠΌΠΈΠΊΡΠΎΠΎΡΠ³Π°Π½ΠΈΠ·ΠΌΠ°ΠΌΠΈ. Π Π°ΡΠΊΡΡΠ²Π°Π΅ΡΡΡ Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΠ΅ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ ΠΌΠ΅ΡΠ°Π³Π΅Π½ΠΎΠΌΠ½ΡΡ
Π΄Π°Π½Π½ΡΡ
Π΄Π»Ρ ΠΏΡΠΎΡΠΈΠ»Π°ΠΊΡΠΈΠΊΠΈ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠΎΠ½Π½ΡΡ
Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΠΉ ΠΈ ΠΏΠΎΡΠ»Π΅ΠΎΠΏΠ΅ΡΠ°ΡΠΈΠΎΠ½Π½ΡΡ
ΠΎΡΠ»ΠΎΠΆΠ½Π΅Π½ΠΈΠΉ ΠΏΡΠΈ ΡΠ΅ΠΊΠΎΠ½ΡΡΡΡΠΊΡΠΈΠ²Π½ΡΡ
Π²ΠΌΠ΅ΡΠ°ΡΠ΅Π»ΡΡΡΠ²Π°Ρ
. ΠΠ΅ΡΠΎΠ΄Ρ ΡΠΎΡ
ΡΠ°Π½Π΅Π½ΠΈΡ Π³ΠΎΠΌΠ΅ΠΎΡΡΠ°Π·Π° ΡΠΊΠΎΡΠΈΡΡΠ΅ΠΌΡ Π½ΠΎΡΠΌΠ°Π»ΡΠ½ΠΎΠΉ Π±ΠΈΠ»ΠΈΠ°ΡΠ½ΠΎΠΉ ΠΌΠΈΠΊΡΠΎΠ±ΠΈΠΎΡΡ ΠΌΠΎΠ³ΡΡ Π±ΡΡΡ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½Ρ Π΄Π»Ρ ΠΏΡΠ΅Π΄ΠΎΡΠ²ΡΠ°ΡΠ΅Π½ΠΈΡ Π³Π΅ΠΏΠ°ΡΠΎΠ±ΠΈΠ»ΠΈΠ°ΡΠ½ΡΡ
Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΠΉ ΠΈ Π»Π΅ΡΠ΅Π½ΠΈΡ Π²ΠΎΡΠΏΠ°Π»ΠΈΡΠ΅Π»ΡΠ½ΡΡ
Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΠΉ ΠΆΠ΅Π»ΡΠ΅Π²ΡΠ²ΠΎΠ΄ΡΡΠΈΡ
ΠΏΡΡΠ΅ΠΉ
Co-growth of Stem Cells With Target Tissue Culture as an Easy and Effective Method of Directed Differentiation
The long-term co-culture of mouse embryonic stem cells (mESC) with rat endothelial cells (EC) was tested for contact differentiation into the endothelial lineage. Serial passaging of rat ECs mixed with mESC in ratio 10:1 resulted in the emergence of a homogeneous cell population expressing mouse endothelial surface markers CD102, CD29, CD31. Rat endothelial surface marker RECA-1 completely disappeared from the co-cultured population after 2 months of weekly passaging. Co-incubation of mESC with rat ECs without cell-to-cell contact did not result in the conversion of mESC into ECs. After co-cultivation of adult mesenchymal stem cells from human endometrium (eMSC) with pre-hepatocyte-like cells of human hepatocarcinoma Huh7 the resulting co-culture expressed mature liver markers (oval cell antigen and cytokeratin 7), none of which were expressed by any of co-cultivated cultures, thus proving that even an immature (proliferating) pre-hepatocyte-like line can induce hepatic differentiation of stem cells. In conclusion, we have developed conditions where long-term co-proliferation of embryonic or adult SC with fully or partially differentiated cells results in stem cell progeny expressing markers of target tissue. In the case of endothelial differentiation, the template population quickly disappeared from the resulted culture and the pure endothelial population of stem cell progeny emerged. This approach demonstrates the expected fate of stem cells during various in vivo SC-therapies and also might be used as an effective in vitro differentiation method to develop the pure endothelium and, potentially, other tissue types of desirable genetic background. Β© Copyright Β© 2021 Kovina, Dyuzheva, Krasheninnikov, Yakovenko and Khodarovich
Misdiagnosis of diabetic foot ulcer in patients with undiagnosed skin malignancies
A growing number of studies report dermal malignancies mimicking diabetic foot ulcers (DFUs). We reviewed clinical cases reporting malignant tumours misdiagnosed to be DFU aiming to identify factors contributing to misdiagnosis. We systematically searched in PubMed for clinical cases reporting on misdiagnosis of DFU in patients with cancer. A chi-square analysis was conducted to show the link between the incidence of initial DFU misdiagnosis and patient age, gender and wound duration. Lesions misdiagnosed to be DFU were subsequently diagnosed as melanoma (68% of the cases), Kaposi's sarcoma (14%), squamous cell carcinoma (11%), mantle cell lymphoma, and diffuse B-cell lymphoma (both by 4%). Older age (β₯65 years) was associated with a significantly increased risk of malignancy masked as DFU (OR: 2.452; 95% CI: 1.132 to 5.312; P valueΒ =.019). The risk of such suspicion in older patients (age β₯ 65 years) was 145% higher than in younger patients (age < 65 years). Clinicians should maintain a high level of awareness towards potentially malignant foot lesions in elderly patients with diabetes (age β₯ 65). Β© 2021 The Authors. International Wound Journal published by Medicalhelplines.com Inc (3M) and John Wiley & Sons Ltd
Bioengineered Bile Duct: the project resume and state of the art in 2018
Aim. Development of the physiologically relevant tissue-engineered graft for repair of bile duct injures (Dyuzheva et al., 2016). <br><br>Conclusions. Designed scaffold showed no cytotoxicity, both BM-MSCs and cholangiocytes migrated into the depth of fibrous material. The constructs was biodegradable in various model mediums: deionized water, phosphate buffer, bile, full culture medium. The next step is pre-clinical trials on rabbits and minipigs for assessment of implantation safety and efficacy. We suppose that this tubular multilayered tissue-engineered construct will be capable to integration in native tissues after implantation and may be used to injured bile duct reparation
Russian consensus on exo-and endocrine pancreatic insufficiency after surgical treatment
The Russian consensus on exo- and endocrine pancreatic insufficiency after surgical treatment was prepared on the initiative of the Russian Pancreatic Club using the Delphi method. Its goal was to consolidate the opinions of national experts on the most relevant issues of diagnosis and treatment of exo- and endocrine insufficiency after surgical interventions on the pancreas. An interdisciplinary approach is ensured by the participation of leading gastroenterologists and surgeons