MORPHOLOGY AND SKELETAL DEVELOPMENT OF RAT (Rattus norvegicus) FETUS AFTER FED DIETS CONTAINING Calliandra calothyrsus LEAF DURING GESTATION PERIOD

This study aims to determine fetus morphology and skeleton development of rats after feddiet containingCalliandra leaf flour. This study used a completely randomized design with 16 pregnant rats that were dividedinto four treatment groups i.e. 0, 10, 17.5 and 25% of Calliandra leaf flour. The diets were given 20 g/rat/dayeach, twice a day, for 30 days (7 days of diet adaptation, 21 days of gestation period, and one day after birth).One day after the birth, all fetuses were separated from their mother for further preparation and analysis. Thedata were analyzed statistically by using SPSS 22 version. If they were distributed normally, the data would beanalyzed usingOneway ANOVA and Duncan Multiple Range Test (DMRT) to see the difference among the groups.Diets containing Calliandra leaf flour fed on pregnant rats decreased weight and length of fetuses and increasedfetus hemorrhage. However, it did not cause dead fetus or decreased the number of life fetuses. Diets containingCalliandra leaf flourfed to pregnant rats did not affect the ossification of vertebrae, costae and sternebrae. However,it inhibited ossification of metacarpus, metatarsus and vertebrae caudalis

PENENTUAN JENIS DAN KADAR ZAT PEWARNA MERAH PADA MAKANAN YANG BEREDAR DI SEKOLAH DASAR DI KELURAHAN JIMBARAN, KECAMATAN KUTA SELATAN, KABUPATEN BADUNG

Penelitian bertujuan untuk mengetahui jenis zat pewarna merah yang dominan dan kadar zat pewarna merah pada makanan yang beredar di Sekolah Dasar di Jimbaran. Penelitian dilakukan dari bulan Agustus-September 2012. Total jumlah sampel yang diambil adalah 16 sampel makanan yang berasal dari 8 sekolah yang mana 2 sampel diambil dari masing-masing makanan. Analisa dilakukan dengan 2 metode uji yaitu uji kualitatif untuk menentukan jenis pewarna dengan kertas kromatografi dan uji kuantitatif untuk menentukan kadar zat pewarna sintetis menggunakan metode spektrofotometri. Hasil penelitian menunjukkan bahwa terdapat 3 jenis zat pewarna makanan yang digunakan yaitu Rhodamin B, Allura Red dan Ponceau 4R. Kadar rata-rata pewarna tertinggi terdapat pada Ponceau 4R (10,046 ppm) dan terendah terdapat pada Allura Red (6,4456 ppm). Konsentrasi tersebut masih berada dibawah batas maksimum penggunaan pewarna makanan yang ditetapkan oleh pemerintah yaitu 70-300 ppm

STRUKTUR HISTOLOGI TRAKEA TIKUS PUTIH (Rattus sp.) YANG TERPAPAR ASAP ROKOK SETELAH DIBERI EKSTRAK BUAH JUWET (Syzygium cumini L.)

This study aimed to determine the effect of juwet fruit extract on histological structure of rat(Rattus sp)  trachea which exposed to cigarette smoke. This research used Completely Randomized Design (CRD), with four treatments: the control group (K0) treated with 0.5 % CMC–Na, (K1) group is exposed to cigarette smoke, (K2) group were given juwetfruit extract, and (K3) group is exposed to cigarette smoke and juwet fruit extracts. Each treatment consisted of 6 rats as replication. The exposure to cigarette smoke is given from  an aerator pump lit cigarettes. Juwet fruit extract and 0.5 % CMC - Na was orally administered (gavage method) for 48 days. The existence of comperative descriptive observed cilia. And goblet number, high epithelium and lumen diameter Data were analyzed with ANOVA and If they were 5 % significantly different would be followed by Duncan test. Results showed that the extract of the fruit juwet significant effect on the histological structure of the trachea mice that had been exposed to smoke

Kualitas Spermatozoa Mencit yang Terpapar Radiasi Sinar-X Secara Berulang (SPERMATOZOA QUALITY OF MICE EXPOSED TO X-RAYS RADIATION IN REPEATED)

In radiology, X-ray has been used to diagnose disease and therapy. However, behind the technologybenefits provided by the radiation, the negative effects are often debated. The purpose of this study was toinvestigate the effects of repeated radiation on sperm quality mice (Mus musculus L). Thirty- two adultmale mice aged three months were divided into groups P1 (1x 200 rad), P2 (2x200 rad), P3 (3x200 rad) andcontrol irradiated with x-rays according to the experimental design . Spermatozoa quality parametersobserved were : number of spermatozoa, motility, viability and morphology of spermatozoa. The results ofthe Post Hoc LSD tests for significant differences (P>0.05) between the control and treatment showed thatthe X-ray radiation exposure to 1x200 rad, 2x200 rad, and 3x200 rad decreases the motility, viability,normal morphology and number spermatozoa produced compared with controls. This is caused by exposureto X-ray radiation causes the formation of free radicals in the body that damage sperm cells mice. Exposureto X-ray radiation repeatedly lowered the quality of spermatozoa of mice