Transcription termination factor Rho prefers catalytically active elongation complexes for releasing RNA

RNA polymerase pauses at different DNA sequences during transcription elongation, and this pausing is associated with distinct conformational state(s) of the Elongation Complex (EC). Transcription termination by the termination factor Rho, an RNA-dependent molecular motor, requires pausing of the EC in the termination zone of Rho-dependent terminators. We hypothesized that the conformational state(s) of the EC associated with this pausing would influence the action of Rho. Analyses of the pausing behavior of the EC at the termination points of two well known Rho-dependent terminators revealed that Rho prefers actively transcribing complexes for termination. RNA release kinetics from stalled ECs showed that the rate of RNA release by Rho was reduced if the EC was irreversibly backtracked, if its RNA exit channel was modified by an RNA hairpin, or the bridge helix/trigger loop movement in its active site was perturbed. These defects were overcome significantly by enhancing the rate of ATP hydrolysis either by increasing the concentration of ATP or by using a Rho mutant with higher ATPase activity. We propose that the force generated from ATP hydrolysis of Rho is the key factor in dislodging the EC through its molecular motor action, and this process is facilitated when the EC is in a catalytically competent state, undergoing rapid “Brownian ratchet” motion

Jesu li kolebanja piona u relativističkim i ultrarelativističkim nuklearnim sudarima ovisna o ljestvici?

Fluctuation pattern of pions is investigated in a wide range of projectile energy from 4.5 AGeV (24Mg-AgBr interactions) to 200 AGeV (32S-AgBr interactions). Two-dimensional analysis is performed. To obtain the correct phase-space partition condition considering anisotropy of phase space, we use the concept of Hurst exponent H. The analysis is performed in a rigorous way by fitting one-dimensional factorial moment saturation curves. The effective fluctuation strength aeff is calculated. The study reveals that the fluctuation pattern is scale-dependent at both relativistic and ultrarelativistic energies.Proučavamo kolebanje piona nastalih u sudarima 24Mg-AgBr na 4.5 AGeV i 32SAgBr na 200 AGeV za široko područje energije. Načinili smo dvodimenzijske analize. Zbog anizotropije faznog prostora, primijenili smo Hurstov eksponent H radi postizanja ispravne razdjele faznog prostora. Proveli smo strog račun prilagodbe jednodimenzijskih krivulja zasićenja faktorijalnih momenata. Izračunali smo efektivnu jakost kolebanja αeff. Našli smo ovisnost slike kolebanja o ljestvici za relativističke i ultrarelativističke energije

Conserved number fluctuations under global rotation in a hadron resonance gas model

Net-charge, net-strangeness and net-baryon number fluctuations measured in ultra-relativistic heavy-ion collisions may reveal details and insights into the quark-hadron transition, hadrochemical freeze-out and possibly aid in the search of the QCD critical point. By controlling the collision energy, some current and upcoming heavy-ion facilities aim to study high energy nucleus-nucleus collisions in the finite net-baryon density regime where the effects of rapid global rotation are also expected to be strong for the peripheral collisions. We discuss the ratios of conserved number susceptibilities that are experimentally measurable via products of the moments of the corresponding distributions and compute the relevant theoretical results in the framework of a rotating hadron resonance gas (rHRG) model.Comment: 7 pages, 5 figure

Mnogofraktalnost i tvorba sporih čestica u sudarima 24Mg–AgBr na energiji 4.5 AGeV

We investigated the multifractality of target fragments of 24Mg-AgBr interaction at low energy (4.5 AGeV) in emission angle phase space, using a new method as proposed by Takagi. The analysis involves the step of measuring the generalised dimension Dq, which in turn deducts the multifractal behaviour of target fragments. We also determine the multifractal specific heat.Istražujemo mnogofraktalnost izlaznih sporih čestica u sudarima 24Mg–AgBr na niskoj energiji (4.5 AGeV) u faznom prostoru kuta emisije, primjenom nove metode koju je predložio Takagi. U analizi se određuje poopćena dimenzija Dq iz koje se izvode mnogofraktalna svojstva izlaznih čestica. Također izvodimo mnogofraktalnu specifičnu toplinu

Multifractality and multifractal specific heat in fragmentation process in

We have investigated the multifractality of target fragments of 24Mg-AgBr interaction at low energy (4.5AGeV) using a new method as proposed by Takagi. The analysis involves the step of measuring the generalised dimension Dq, which in turn, deduce the multifractal behaviour of target fragments. Ultimately we determine multifractal specific heat. A comparison with other data is also done.Author Affiliation: Dipak Ghosh, Argha Deb, Keya Dutta (Chattopadhyay), Rinku Sarkar, Ishita Dutta and Mitali Mondal 1.Nuclear and Particle Physics Research Centre, Department of Physics, Jadavpur University, Kolkata-700 032, India E-mail : [email protected] and Particle Physics Research Centre, Department of Physics, Jadavpur University, Kolkata-700 032, Indi

N7-(carboxymethyl)guanine-lithium crystalline complex: a bioinspired solid electrolyte

Electrochemical device with components having direct significance to biological life processes is a potent futuristic strategy for the realization of all-round green and sustainable development. We present here synthesis design, structural analysis and ion transport of a novel solid organic electrolyte (G7Li), a compound reminiscent of ion channels, derived from regioisomeric N7-guanine-carboxylate conjugate and Li-ions. G7Li, with it\u27s in-built supply of Li(+)-ions, exhibited remarkably high lithium-ion transference number (= 0.75) and tunable room temperature ionic conductivity spanning three decades (≈10(-7) to 10(-3) Ω(-1) cm(-1)) as a function of moisture content. The ionic conductivity show a distinct reversible transition around 80-100 °C, from a dual Li(+) and H(+) (<100 °C) to a pure Li(+) conductor (>100 °C). Systematic studies reveal a transition from water-assisted Li-ion transport to Li hopping-like mechanism involving guanine-Li coordination. While as-synthesized G7Li has potential in humidity sensors, the anhydrous G7Li is attractive for rechargeable batteries