1 research outputs found
Enzyme Recycling by Adsorption during Hydrolysis of Oxygen-Delignified Wheat Straw
Enzyme recycling
by adsorption from supernatant to fresh substrate
is a promising strategy to reduce enzyme expenses and the production
cost of lignocellulosic ethanol. The study was performed using oxygen-delignified
wheat straw, and the effect of lignin content, enzyme loading, and
hydrolysis time on recycling was determined. The percent of recycled
cellulases, 0β35% of initial cellulase loading, increased with
increasing enzyme loading and hydrolysis time but decreased with increasing
lignin content. Cellulose conversions of 10β71% were achieved
during the second hydrolysis round using only recycled cellulases
indicating the existence of a highly active subset of enzymes. To
achieve constant production of sugars during enzyme recycling, fresh
cellulases were loaded before the second hydrolysis round to match
the cellulase loading used in the first round. Subsequently, similar
glucose, xylose, and protein concentrations were obtained at the end
of the first and second rounds for all conditions. Recycling mass
balances were developed to support future techno-economic analyses
to determine the impact of enzyme recycling on the cost of ethanol