Protein Encapsulation Using Complex Coacervates: What Nature Has to Teach Us

Protein encapsulation is a growing area of interest, particularly in the fields of food science and medicine. The sequestration of protein cargoes is achieved using a variety of methods, each with benefits and drawbacks. One of the most significant challenges associated with protein encapsulation is achieving high loading while maintaining protein viability. This difficulty is exacerbated because many encapsulant systems require the use of organic solvents. By contrast, nature has optimized strategies to compartmentalize and protect proteins inside the cell—a purely aqueous environment. Although the mechanisms whereby aspects of the cytosol is able to stabilize proteins are unknown, the crowded nature of many newly discovered, liquid phase separated “membraneless organelles” that achieve protein compartmentalization suggests that the material environment surrounding the protein may be critical in determining stability. Here, encapsulation strategies based on liquid–liquid phase separation, and complex coacervation in particular, which has many of the key features of the cytoplasm as a material, are reviewed. The literature on protein encapsulation via coacervation is also reviewed and the parameters relevant to creating protein‐containing coacervate formulations are discussed. Additionally, potential opportunities associated with the creation of tailored materials to better facilitate protein encapsulation and stabilization are highlighted

Appraisal of Nuclear Energy as an Alternative Option in South Africa’s Energy Scenario: A Multicriteria Analysis

South Africa is being confronted with an irregular power supply, leading to persistent load shedding due to aged and unreliable coal-fired power plants. Connected with coal as a generating source for electricity from fossil fuels are environmental concerns such as emissions of greenhouse gases and climate change impacts. Nuclear energy can allay the country’s dependence on coal as a source of energy. This article, therefore, reviews the feasibility of nuclear energy using a multicriteria analysis technique. A combination of Strengths, weaknesses, Opportunities, and Threats (SWOT) analysis and Analytical Hierarchy Process (AHP) was used to evaluate the external and internal factors that could either positively or negatively affect the country’s nuclear energy expansion drive. From the analysis, the country’s enabling laws and regulatory framework recorded the highest score of 39.2% under the strengths for the sector. In the case of the weaknesses, the high cost of construction and long construction framework recorded the highest weight, of 50.47%. Energy export and demand under the opportunities recorded a weight of 52.09%, ranking it as the highest opportunity for the sector. Seismic events were identified as the biggest threat for nuclear power expansion in the country, and the experts assigned a weight of 42.5% to this factor. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.Acknowledgments: The authors acknowledge with thanks the Centre for Postgraduate Studies and the Postdoctoral Fellowship/Research Committee of the Cape Peninsula University of Technology, and the Directorate of Research and Innovations of the University of Venda