ラット カイバ ウチガワ ゼントウゼン ヒシツ オヨビ センジョウタイ ニオケル タイシャ プロファイル ノ カレイ ヘンカ

学位授与機関：　滋賀医科大学　博士（医学）甲第796号　平成30年3月9日Biochemical and Biophysical Research Communications, 2017, 493(3), p.1356-1363

Modulation of Proteome Profile in AβPP/PS1 Mice Hippocampus, Medial Prefrontal Cortex, and Striatum by Palm Oil Derived Tocotrienol-Rich Fraction.

Tocotrienol-rich fraction (TRF) is a mixture of vitamin E analogs derived from palm oil. We previouslydemonstrated that supplementation with TRF improved cognitive function and modulated amyloid pathology in AβPP/PS1 mice brains. The current study was designed to examine proteomic profiles underlying the therapeutic effect of TRF in the brain. Proteomic analyses were performed on samples of hippocampus, medial prefrontal cortex (mPFC), and striatum using liquid chromatography coupled to Q Exactive HF Orbitrap mass spectrometry. From these analyses, we profiled a total of 5,847 proteins of which 155 proteins were differentially expressed between AβPP/PS1 and wild-type mice. TRF supplementation of these mice altered the expression of 255 proteins in the hippocampus, mPFC, and striatum. TRF also negatively modulated the expression of amyloid beta A4 protein and receptor-type tyrosine-protein phosphatase alpha protein in the hippocampus. The expression of proteins in metabolicpathways, oxidative phosphorylation, and those involved in Alzheimer\u27s disease were altered in the brains of AβPP/PS1 mice that received TRF supplementation