Effect of Swine Manure Pit Additives and Facility Disinfectants on the Fate of Antibiotics and Manure Composition During Simulated Swine Manure Slurry Storage

This thesis investigates the effect of time and of swine manure slurry treatment on the physical properties, nutrient content, and the concentrations of antibiotics chlortetracycline, lincomycin, and tiamulin in simulated storage of swine manure. In one experiment the slurry was treated with six additive products. In a second experiment a set of four disinfectant products were used. Control consisted of unamended slurry. Manure was stored in 60 liter stainless steel bioreactors to simulate deep pit storage and was sampled 7 times over a 40 day incubation. From an ANOVA of the results, it was concluded that evaporation may be contributing a significant effect in concentration change of the manure constituents. With this in mind, each time series was normalized by its final time point and a second ANOVA was performed along with a growth curve analysis on the means and slopes over time of the normalized data. This further analysis resulted in a large decrease in treatment effects in the additives experiment but demonstrated no reduction in treatment effects in the disinfectants experiment. With the additives experiment the Coban 90 treatment produced an increase in mean total suspended solids relative to the control. Five of the additive products produced a slower decrease in electrical conductivity relative to the control. Only one additive treatment was found to produce a greater mean tiamulin concentration relative to the control over the course of the experiment. With the disinfectants experiment, two treatments increased mean concentrations of total nitrogen. Another two treatments caused an increase in mean phosphorus concentration. Once again, only one treatment produced an increase in mean tiamulin concentrations. The antibiotics concentrations reported in both experiments were highly variable and could not be fit accurately to decay models. Advisors: Shannon Bartelt-Hunt and Amy Schmid

Antibiotic resistance genes in swine manure slurry as affected by pit additives and facility disinfectants

Manure storage facilities are critical control points to reduce antibiotic resistance genes (ARGs) in swine manure slurry before the slurry is land applied. However, little is known about how exogenous chemicals entering the manure storage facilities may affect the fate of ARGs. The objective of this study was to analyze the impact of six commonly used pit additives and four facility disinfectants on the concentration of ARGs in swine manure slurry. Bench scale reactors, each containing approximately 50 L of liquid swine manure, were dosed with additives or disinfectants and were sampled for 40 days. Seven antibiotic resistance genes along with the intI1 gene and the 16S rRNA gene were monitored. Out of the six additives tested, Sludge Away significantly reduced the time-averaged absolute abundance of erm(C), erm(F), tet(Q), and the 16S rRNA gene as compared to the no additive control. Out of the four disinfectants tested, Tek-Trol significantly reduced the time-averaged absolute abundance of erm(B), erm(C), erm(F), intI1, tet(Q), and tet(X) than did the no-disinfectant control. According to Spearman\u27s rank correlation, three genes erm(F), tet(Q), and tet(X) showed a strong to perfectly positive correlation and the two genes erm(B) and tet(O) showed a moderate to strong correlation in both the additive and disinfectant tests. Overall, the disinfectants were more effective in controlling the absolute abundance of ARGs than were the pit additives

Effect of Swine Manure Pit Additives and Facility Disinfectants on the Fate of Antibiotics and Manure Composition During Simulated Swine Manure Slurry Storage

This thesis investigates the effect of time and of swine manure slurry treatment on the physical properties, nutrient content, and the concentrations of antibiotics chlortetracycline, lincomycin, and tiamulin in simulated storage of swine manure. In one experiment the slurry was treated with six additive products. In a second experiment a set of four disinfectant products were used. Control consisted of unamended slurry. Manure was stored in 60 liter stainless steel bioreactors to simulate deep pit storage and was sampled 7 times over a 40 day incubation. From an ANOVA of the results, it was concluded that evaporation may be contributing a significant effect in concentration change of the manure constituents. With this in mind, each time series was normalized by its final time point and a second ANOVA was performed along with a growth curve analysis on the means and slopes over time of the normalized data. This further analysis resulted in a large decrease in treatment effects in the additives experiment but demonstrated no reduction in treatment effects in the disinfectants experiment. With the additives experiment the Coban 90 treatment produced an increase in mean total suspended solids relative to the control. Five of the additive products produced a slower decrease in electrical conductivity relative to the control. Only one additive treatment was found to produce a greater mean tiamulin concentration relative to the control over the course of the experiment. With the disinfectants experiment, two treatments increased mean concentrations of total nitrogen. Another two treatments caused an increase in mean phosphorus concentration. Once again, only one treatment produced an increase in mean tiamulin concentrations. The antibiotics concentrations reported in both experiments were highly variable and could not be fit accurately to decay models. Advisors: Shannon Bartelt-Hunt and Amy Schmid

Swine slurry characteristics as affected by selected additives and disinfectants

Current swine industry practice is to house animals in confinement facilities which capture and store feces and urine as slurry in pits below the production area. Additives and disinfectants may be introduced into the manure pits. This study was conducted to measure the effects of additives and disinfectants on temporal changes in swine slurry characteristics. Slurry from a commercial swine production facility in southeast Nebraska, USA was collected and transferred to 57 L reactors located within a greenhouse. Selected additives and disinfectants were added to the reactors and physical properties, chemical characteristics, and antibiotic concentrations were monitored for 40 days. Concentrations of dry matter (DM), total nitrogen (TN), phosphorus pentoxide (P2O5), calcium (Ca), magnesium (Mg), zinc (Zn), iron (Fe), manganese (Mn), and copper (Cu) were significantly greater than the Control in each of the reactors containing additives. The reactors in which the additives MOC-7, More Than Manure®, Sludge Away, and Sulfi-Doxx were introduced had significantly greater values of chemical oxygen demand (COD), total volatile solids (TVS), total suspended solids (TSS), total solids (TS), dry matter (DM), TN, P2O5, Ca, Mg, Zn, Fe, Mn, Cu and chlortetracycline than the other additive treatments. Concentrations of TVS and TSS were significantly lower in the reactors containing Clorox® and Virkon™ than the other disinfectant treatments. The total dissolved solids (TDS) concentration of 26,500 mg/ L and pH value of 7.27 obtained for the reactors containing Tek-Trol were significantly greater than measurements obtained for the other treatments. Concentrations of chlortetracycline and tiamulin of 8840 and 28.8 ng/g , respectively, were significantly lower for the treatments containing Tek-Trol. The sodium (Na) concentration of 1070 mg/ Lmeasured in the reactors containing Clorox® was significantly greater than values for the other disinfectant treatments. The introduction of selected additives and disinfectants may influence certain physical properties, chemical characteristics, and antibiotic concentrations of swine slurry

Swine slurry characteristics as affected by selected additives and disinfectants

Current swine industry practice is to house animals in confinement facilities which capture and store feces and urine as slurry in pits below the production area. Additives and disinfectants may be introduced into the manure pits. This study was conducted to measure the effects of additives and disinfectants on temporal changes in swine slurry characteristics. Slurry from a commercial swine production facility in southeast Nebraska, USA was collected and transferred to 57 L reactors located within a greenhouse. Selected additives and disinfectants were added to the reactors and physical properties, chemical characteristics, and antibiotic concentrations were monitored for 40 days. Concentrations of dry matter (DM), total nitrogen (TN), phosphorus pentoxide (P2O5), calcium (Ca), magnesium (Mg), zinc (Zn), iron (Fe), manganese (Mn), and copper (Cu) were significantly greater than the Control in each of the reactors containing additives. The reactors in which the additives MOC-7, More Than Manure®, Sludge Away, and Sulfi-Doxx were introduced had significantly greater values of chemical oxygen demand (COD), total volatile solids (TVS), total suspended solids (TSS), total solids (TS), dry matter (DM), TN, P2O5, Ca, Mg, Zn, Fe, Mn, Cu and chlortetracycline than the other additive treatments. Concentrations of TVS and TSS were significantly lower in the reactors containing Clorox® and Virkon™ than the other disinfectant treatments. The total dissolved solids (TDS) concentration of 26,500 mg L–1 and pH value of 7.27 obtained for the reactors containing Tek-Trol were significantly greater than measurements obtained for the other treatments. Concentrations of chlortetracycline and tiamulin of 8840 and 28.8 ng g–1, respectively, were significantly lower for the treatments containing Tek-Trol. The sodium (Na) concentration of 1070 mg L–1 measured in the reactors containing Clorox® was significantly greater than values for the other disinfectant treatments. The introduction of selected additives and disinfectants may influence certain physical properties, chemical characteristics, and antibiotic concentrations of swine slurry

Swine slurry characteristics as affected by selected additives and disinfectants

Current swine industry practice is to house animals in confinement facilities which capture and store feces and urine as slurry in pits below the production area. Additives and disinfectants may be introduced into the manure pits. This study was conducted to measure the effects of additives and disinfectants on temporal changes in swine slurry characteristics. Slurry from a commercial swine production facility in southeast Nebraska, USA was collected and transferred to 57 L reactors located within a greenhouse. Selected additives and disinfectants were added to the reactors and physical properties, chemical characteristics, and antibiotic concentrations were monitored for 40 days. Concentrations of dry matter (DM), total nitrogen (TN), phosphorus pentoxide (P2O5), calcium (Ca), magnesium (Mg), zinc (Zn), iron (Fe), manganese (Mn), and copper (Cu) were significantly greater than the Control in each of the reactors containing additives. The reactors in which the additives MOC-7, More Than Manure®, Sludge Away, and Sulfi-Doxx were introduced had significantly greater values of chemical oxygen demand (COD), total volatile solids (TVS), total suspended solids (TSS), total solids (TS), dry matter (DM), TN, P2O5, Ca, Mg, Zn, Fe, Mn, Cu and chlortetracycline than the other additive treatments. Concentrations of TVS and TSS were significantly lower in the reactors containing Clorox® and Virkon™ than the other disinfectant treatments. The total dissolved solids (TDS) concentration of 26,500 mg/ L and pH value of 7.27 obtained for the reactors containing Tek-Trol were significantly greater than measurements obtained for the other treatments. Concentrations of chlortetracycline and tiamulin of 8840 and 28.8 ng/g , respectively, were significantly lower for the treatments containing Tek-Trol. The sodium (Na) concentration of 1070 mg/ Lmeasured in the reactors containing Clorox® was significantly greater than values for the other disinfectant treatments. The introduction of selected additives and disinfectants may influence certain physical properties, chemical characteristics, and antibiotic concentrations of swine slurry