A regression method for EEG-based cross-dataset fatigue detection

Introduction: Fatigue is dangerous for certain jobs requiring continuous concentration. When faced with new datasets, the existing fatigue detection model needs a large amount of electroencephalogram (EEG) data for training, which is resource-consuming and impractical. Although the cross-dataset fatigue detection model does not need to be retrained, no one has studied this problem previously. Therefore, this study will focus on the design of the cross-dataset fatigue detection model.Methods: This study proposes a regression method for EEG-based cross-dataset fatigue detection. This method is similar to self-supervised learning and can be divided into two steps: pre-training and the domain-specific adaptive step. To extract specific features for different datasets, a pretext task is proposed to distinguish data on different datasets in the pre-training step. Then, in the domain-specific adaptation stage, these specific features are projected into a shared subspace. Moreover, the maximum mean discrepancy (MMD) is exploited to continuously narrow the differences in the subspace so that an inherent connection can be built between datasets. In addition, the attention mechanism is introduced to extract continuous information on spatial features, and the gated recurrent unit (GRU) is used to capture time series information.Results: The accuracy and root mean square error (RMSE) achieved by the proposed method are 59.10% and 0.27, respectively, which significantly outperforms state-of-the-art domain adaptation methods.Discussion: In addition, this study discusses the effect of labeled samples. When the number of labeled samples is 10% of the total number, the accuracy of the proposed model can reach 66.21%. This study fills a vacancy in the field of fatigue detection. In addition, the EEG-based cross-dataset fatigue detection method can be used for reference by other EEG-based deep learning research practices

Study on Rules of Fault Stress Variation Based on Microseismic Monitoring and Numerical Simulation at the Working Face in the Dongjiahe Coal Mine

Microseismic monitoring technology was used to study the real-time evolution of rock mass damage generated by a working face as it approached a fault in Dongjiahe Coal Mine. The influence of vertical zoning of overlying strata on damage at the fault was analyzed. Numerical simulation using finite element method based on meso-statistical damage theory was used to investigate the nonlinear and nonuniform failure behaviour of the rock mass near the fault. The response of the fault stress to excavation activity and the rule of fault activation were examined. The results show that the fault damage has segmental characteristics. Microcracks are first generated at the fractured zone that is divided into lower, middle, and upper sections, located 30∼70 m, 120∼180 m, and 230∼280 m above the coal seam, respectively. There was also a segmentation phenomenon in the stress response of fault. The risk of fault activation was evaluated by using the ratio of shear stress to the maximum principal stress. When the working face was 260 m and 140 m away from the fault, the activation risk at the upper-middle and lower sections began to increase, respectively. When the fault was within 60 m, the risk of fault activation was highest

Study on a Large-Scale Persistent Strong Dense Fog Event in Central and Eastern China

A large-scale persistent strong dense fog (SDF) event that occurred from December 30, 2016, to January 5, 2017, in central and eastern China is analyzed by using a variety of data, including high-resolution satellite and surface observations, meteorological tower observations, fine-resolution sounding observations, and NCEP/NCAR reanalysis data. The results show the following: (1) The SDF event has the characteristics of long duration, wide influence range, large intensity, and serious air pollution. During the study period, there are 531 stations with SDF events, covering an area of over 360,000 km2. There were five stations in Hebei province where the fog lasted for more than 77 hours, and even some stations did not dissipate during the day. (2) Radiation fog and advection fog alternate in this SDF event, namely, radiation fog (20:00 BT on December 30 to 14:00 on January 1), advection radiation fog (20:00 on January 1 to 08:00 on January 2), radiation fog (night on January 2 to daytime on January 3), and advection radiation fog (night on January 3 to January 5). The characteristic of radiation fog is that the central and eastern part of China was controlled by “L” type high pressure. In the stage of advection radiation fog, the combined effect of weak cold advection and radiation cooling leads to the occurrence of SDF. (3) Regarding the duration of the fog event, the inversion structure is continuously maintained at night and in the morning near the stratum, and when the fog intensity is strong, the inversion intensity is correspondingly large, the fog top is lower than the inversion layer top, and the top of the SDF is between 80 and 400 meters

The role of cytochrome c on apoptosis induced by Anagrapha falcifera multiple nuclear polyhedrosis virus in insect Spodoptera litura cells.

There are conflicting reports on the role of cytochrome c during insect apoptosis. Our previous studies have showed that cytochrome c released from the mitochondria was an early event by western blot analysis and caspase-3 activation was closely related to cytochrome c release during apoptosis induced by baculovirus in Spodoptera litura cells (Sl-1 cell line). In the present study, alteration in mitochondrial morphology was observed by transmission electron microscopy, and cytochrome c release from mitochondria in apoptotic Sl-1 cells induced with Anagrapha falcifera multiple nuclear polyhedrosis virus (AfMNPV) has further been confirmed by immunofluoresence staining protocol, suggesting that structural disruption of mitochondria and the release of cytochrome c are important events during Lepidoptera insect cell apoptosis. We also used Sl-1 cell-free extract system and the technique of RNA interference to further investigate the role of cytochrome c in apoptotic Sl-1 cells induced by AfMNPV. Caspase-3 activity in cell-free extracts supplemented with exogenous cytochrome c was determined and showed an increase with the extension of incubation time. DsRNA-mediated silencing of cytochrome c resulted in the inhibition of apoptosis and protected the cells from AfMNPV-induced cell death. Silencing of expression of cytochrome c had a remarkable effect on pro-caspase-3 and pro-caspase-9 activation and resulted in the reduction of caspase-3 and caspase-9 activity in Sl-1 cells undergoing apoptosis. Caspase-9 inhibitor could inhibit activation of pro-caspase-3, and the inhibition of the function of Apaf-1 with FSBA blocked apoptosis, hinting that Apaf-1 could be involved in Sl-1 cell apoptosis induced by AfMNPV. Taken together, these results strongly demonstrate that cytochrome c plays an important role in apoptotic signaling pathways in Lepidopteran insect cells

Frailty Factors and Outcomes in Patients Undergoing Orthopedic Surgery: Protocol for a Systematic Review and Meta-analysis

BackgroundFrailty is an aggregate expression of susceptibility to adverse health outcomes because of age- and disease-related deficits that accumulate across multiple domains. Previous studies have found the presence of preoperative frailty is associated with an increased risk of adverse outcomes. The number of older adults undergoing orthopedic surgery is rapidly increasing. However, there has been no evidence-based study on the relationship between frailty and outcomes in patients undergoing orthopedic surgery. ObjectiveThe aims of this study are to investigate the association between frailty and outcomes in patients who underwent orthopedic surgery as well as patient factors associated with frailty. MethodsThe methods to be used for this systematic review are reported according to the PRISMA-P (Preferred Reporting Items for Systematic Review and Meta-analysis Protocols) 2015 checklist. An extensive search will be conducted in PubMed, Embase, the Cochrane Library, and other mainstream databases. Any study where patients undergoing orthopedic surgery were assessed using a defined or validated measure of frailty and the association of frailty with patient factors and/or outcomes was reported will be included. A total of 2 researchers will independently screen articles for inclusion, with disagreements resolved by a third reviewer. We will perform a narrative synthesis of the factors associated with frailty, prevalence of frailty, effect of frailty on patient outcomes, and interventions for patients who are frail. A meta-analysis focusing on individual factors associated with frailty and the effect of frailty on patient outcomes will be performed, if applicable. The risk of bias will be evaluated. A subgroup analysis and sensitivity analysis will be performed. ResultsLiterature searches were conducted in September 2021 and the review is anticipated to be completed by the end of July 2022. ConclusionsThis systematic review and meta-analysis will provide an overview of frailty and investigate the relationship between frailty and patient outcomes as well as the relationship between patient factors and frailty in patients undergoing orthopedic surgery. This study could potentially increase patients’ awareness of the outcomes associated with frailty, compel clinical specialties to further acknowledge the concept of frailty, and enhance the development of assessment instruments and tools for frailty. Trial RegistrationPROSPERO CRD42020181846; https://www.crd.york.ac.uk/prospero/display_record.php?RecordID=181846 International Registered Report Identifier (IRRID)DERR1-10.2196/2833

Changes of mitochondria in apoptotic cells induced with AfMNPV under confocal microscopy.

<p>(A) Control cells stained with Mito-Tracker Green, showing mitochondrial normal distribution; (B) Infection of AfMNPV caused mitochondria to aggregate at 4 h post-infection; (C) Mito-Tracker Green staining showed a diffusion of fluorescence at 8 h post-infection, suggesting the disruption and depolarization of mitochondria. Bar = 10 <b>µ</b>m.</p

Pathway of apoptosis mediated by cytochrome c in AfMNPV-infected Sl-1 cells.

<p>MPT: mitochondrial permeability transition.</p

Down-regulation of cytochrome c resulted in the reduction of caspase 3 and caspase-9 activity in AfMNPV-induced Sl-1 cell.

<p>(A) Sl-1 cells treated with dsRNA for different time points, apoptosis was induced with AfMNPV, and then caspase-3 activity was measured at 10 h post-infection.1. Control cells treated alone with AfMNPV; 2. Cells treated with GFP dsRNA and virus; 3. Cells without any treatment; 4. Cells treated with dsRNA, 24 h later, infected by AfMNPV for 10 h; 5. Cells treated alone with dsRNA for 24 h; 6. Cells treated with dsRNA, 48 h later, infected by AfMNPV for 10 h; 7. Cells treated alone with dsRNA for 48 h; 8. Cells treated with dsRNA, 72 h later, infected by AfMNPV for 10 h; 9. Cells treated alone with dsRNA for 72 h. (B) Caspase-9 activity in cytochrome c dsRNA-treated Sl-1 cells after infection with AfMNPV for 10 h, compared with control cells.1. Normal cells; 2. Cells infected with AfMNPV; 3. Cells infected with AfMNPV for 10 h after GFP dsRNA treatment for 48 h; 4. Cells infected with AfMNPV for 10 h after cyt c dsRNA treatment for 48 hr. *, <i>p</i><0.05.</p