Characterization of Human Coronavirus Etiology in Chinese Adults with Acute Upper Respiratory Tract Infection by Real-Time RT-PCR Assays

BACKGROUND: In addition to SARS associated coronaviruses, 4 non-SARS related human coronaviruses (HCoVs) are recognized as common respiratory pathogens. The etiology and clinical impact of HCoVs in Chinese adults with acute upper respiratory tract infection (URTI) needs to be characterized systematically by molecular detection with excellent sensitivity. METHODOLOGY/PRINCIPAL FINDINGS: In this study, we detected 4 non-SARS related HCoV species by real-time RT-PCR in 981 nasopharyngeal swabs collected from March 2009 to February 2011. All specimens were also tested for the presence of other common respiratory viruses and newly identified viruses, human metapneumovirus (hMPV) and human bocavirus (HBoV). 157 of the 981 (16.0%) nasopharyngeal swabs were positive for HCoVs. The species detected were 229E (96 cases, 9.8%), OC43 (42 cases, 4.3%), HKU1 (16 cases, 1.6%) and NL63 (11 cases, 1.1%). HCoV-229E was circulated in 21 of the 24 months of surveillance. The detection rates for both OC43 and NL63 were showed significantly year-to-year variation between 2009/10 and 2010/11, respectively (P<0.001 and P = 0.003), and there was a higher detection frequency of HKU1 in patients aged over 60 years (P = 0.03). 48 of 157(30.57%) HCoV positive patients were co-infected. Undifferentiated human rhinoviruses and influenza (Flu) A were the most common viruses detected (more than 35%) in HCoV co-infections. Respiratory syncytial virus (RSV), human parainfluenza virus (PIV) and HBoV were detected in very low rate (less than 1%) among adult patients with URTI. CONCLUSIONS/SIGNIFICANCE: All 4 non-SARS-associated HCoVs were more frequently detected by real-time RT-PCR assay in adults with URTI in Beijing and HCoV-229E led to the most prevalent infection. Our study also suggested that all non-SARS-associated HCoVs contribute significantly to URTI in adult patients in China

Kinematics of the Tengchong Terrane in SE Tibet from the late Eocene to early Miocene: Insights from coeval mid-crustal detachments and strike-slip shear zones.

It is generally believed that the extrusion of SE Tibet was bounded by the dextral Gaoligong and the sinistral Ailaoshan-Red River strike-slip shear zones from the Oligocene to early Miocene. This study integrates field map- ping, structural analysis and geochronology in western Yunnan (China), where foliated Precambrian basement rocks and late Cretaceous to early Eocene plutons are exposed to the west of the Gaoligong shear zone. We found that late Eocene to early Miocene flat-lying ductile shear zones were kinematically related to steeply dip- ping strike-slip shear zones. Four elongated gneiss domes (Donghe, Guyong, Yingjiang and Sudian) are cored by high-grade metamorphic rocks and pre-kinematic granite plutons, and bounded by top-to-NE detachments and NE-trending dextral strike-slip shear zones. Zircon U-Pb ages from LA-ICP-MS analysis and 40Ar/39Ar ages of micas and hornblende demonstrate that the flat-lying Donghe Detachment (N 35–15 Ma) and the Nabang dextral strike-slip shear zone (41–19 Ma) were sites of prolonged, mostly coeval ductile deformation from amphibolite to greenschist facies metamorphism. The Gaoligong shear zone experienced dextral shearing under similar meta- morphic conditions between 32 and 10 Ma. Consistent 40Ar/39Ar ages of hornblende from the three shear zones indicate their contemporaneity at mid-crustal depth, causing the rapid exhumation and SW-ward extrusion of the Tengchong Terrane. The strain geometry and shear zone kinematics in the Tengchong Terrane are interpreted with folding of the anisotropic lithosphere around a vertical axis, i.e., the northeast corner of the Indian Plate since 41 Ma. The newly discovered NE-trending Sudian, Yingjiang, and Lianghe strike-slip shear zones are subordinate ductile faults accommodating the initially rapid clockwise rotation of the Tengchong Terrane. The detachments caused mid-crustal decoupling and faster SW-ward extrusion below the sedimentary cover, whereas the strike-slip shear zones accommodated extrusion and clockwise rotation of the Tengchong Terrane around the proto-Eastern Himalayan syntaxis since the late Eocene.ISSN:0040-1951ISSN:1879-326

Prevalence and Genetic Diversity Analysis of Human Coronavirus OC43 among Adult Patients with Acute Respiratory Infections in Beijing, 2012

<div><p>To determine the prevalence, epidemiology and genetic diversity of human coronavirus OC43 (HCoV-OC43) among adult patients with acute respiratory infections (ARI) in Beijing,five hundred and fifty-nine nasopharyngeal swab samples were collected from adult patients with ARI in Beijing. The prevalence of HCoV-OC43 infection among these patients was assessed using two different OneStep reverse transcription polymerase chain reaction (RT-PCR) assays. The epidemiological profiles of the patients with HCoV-OC43 infection were described. Partial S and N genes of HCoV-OC43 circulating strains were sequenced followed by phylogenetic analysis and amino acid alignment. Our results showed that the prevalence of HCoV-OC43 infection was 12.52% (95% CI: 9.78–15.26%), and the epidemic peak occurred in autumn. Fifty partial S and 40 partial N fragments were obtained from these patients. Phylogenetic analysis based on neighbour-joining method showed that at least three distinct clusters (A, B, C/D) of HCoV-OC43 strains were circulating among adult patients with ARI in Beijing. In addition, some novel unique clusters (UNT) of HCoV-OC43 were found in the S- and N-based phylogenetic trees. Furthermore, consensus amino acids substitutes for each cluster were also found after alignment of partial S or N sequence coding region in this study. In conclusion, we herein describe the prevalence of HCoV-OC43 among adult patients and provide substantial evidence for the genetic diversity of HCoV-OC43 circulating in Beijing.</p></div

Summary on consensus amino acid changes for each cluster among S coding region of HCoV-OC43.

<p>Summary on consensus amino acid changes for each cluster among S coding region of HCoV-OC43.</p

Frequency of HCoV-OC43 infection, as determined by two molecular assays, and coinfection.

<p>Frequency of HCoV-OC43 infection, as determined by two molecular assays, and coinfection.</p

Improved temporal resolution in ultrafast electron diffraction measurements through THz compression and time-stamping

We present an experimental demonstration of ultrafast electron diffraction (UED) with THz-driven electron bunch compression and time-stamping that enables UED probes with improved temporal resolution. Through THz-driven longitudinal bunch compression, a compression factor of approximately four is achieved. Moreover, the time-of-arrival jitter between the compressed electron bunch and a pump laser pulse is suppressed by a factor of three. Simultaneously, the THz interaction imparts a transverse spatiotemporal correlation on the electron distribution, which we utilize to further enhance the precision of time-resolved UED measurements. We use this technique to probe single-crystal gold nanofilms and reveal transient oscillations in the THz near fields with a temporal resolution down to 50 fs. These oscillations were previously beyond reach in the absence of THz compression and time-stamping

Co-Detections of HCoVs and other Respiratory Virus in the study population.

*<p>Be only counted once in the total.</p

Temporal distribution of the 4 HCoV species between March 2009 and February 2011 inclusive.

<p>Temporal distribution of the 4 HCoV species between March 2009 and February 2011 inclusive.</p