3 research outputs found
Digestibilnost alergoida polena pelina u simuliranim uslovima gastrointestinalnog trakta
Chemically modified allergens (allergoids) have found use in both traditional and novel forms of immunotherapy of allergic disorders. Novel forms of immunotherapy include local allergen delivery, via the gastrointestinal tract. This study conveys the gastrointestinal stability of three types of mugwort pollen allergoids under simulated conditions of the gut. Allergoids of the pollen extract of Artemisia vulgaris were obtained by means of potassium cyanate, succinic and maleic anhydride. Gastrointestinal tract conditions (saliva, and gastric fluid) were simulated in accordance with the EU Pharmacopoeia. The biochemical and immunochemical properties of the derivatives following exposure to different conditions were monitored by determining the number of residual amino groups with 2,4,6-trinitrobenzenesulfonic acid, SDS PAGE, immunoblotting and inhibition of mugwort-specific IgE. Exposure to saliva fluid for 2 min did not influence the biochemical and immunochemical properties of the derivatives. In the very acidic conditions of the simulated gastric fluid, the degree of demaleylation and desuccinylation, even after 4 h exposure, was low, ranging from 10 to 30 %. The digestion patterns with pepsin proceeded rapidly in both the unmodified and modified samples. In all four cases, a highly resistant IgE-binding protein the Mwof which was about 28-35 kD, was present. Within the physiological conditions, no new IgE binding epitopes were revealed, as demonstrated by immunoblot and CAP inhibition of the mugwort specific IgE binding. An important conclusion of this study is the stability of the modified derivatives in the gastrointestinal tract of patients, within physiological conditions. The means that they are suitable for use in much higher concentrations in local forms of immunotherapy than unmodified ones.U ovom radu su prikazani rezultati ispitivanja stabilnosti tri tipa alergoida polena pelina u simuliranom želudaÄnom soku. KoristeÄi kalijum-cijanat anhidrid Äilibarne i anhidrid maleinske kiseline, napravljeni su alergoidi polena pelina (Artemisia vulgaris). Saliva i želudaÄni sok su simulirani na osnovu evropske farmakopeje. Biohemijske i imunohemijske osobine derivata posle izlaganja razliÄitim uslovima, praÄene su: odreÄivanjem broja slobodnih amino grupa u reakciji sa TNBS, SDS PAG elektroforezom, imunoblotom i odreÄivanjem pelin-specifiÄnog imunoglobulina E (IgE). Izlaganje salivi u trajanju od 2 minuta ne utiÄe na biohemijske i imunohemijske osobine derivata. U kiseloj sredini želudaÄnog soka ne dolazi do znaÄajnog demaleilovawa i desukcinilovanja. Äak i posle ÄetvoroÄasovnog izlaganja, taj procenat je u opsegu 10-30 %. Alergoidi pelina se trenutno digestuju pepsinom, sa izuzetkom visoko rezistentne proteinske trake molekulske mase 28-35 kD, koja odgovara važnom IgE-vezujuÄem proteinu polena pelina. Imunoblotom i CAP-inhibicijom je pokazano da, u okviru fizioloÅ”kih uslova, ne dolazi do stvaranja novih IgE-vezujuÄih epitopa. Hemijska stabilnost modifikovanih derivata u simuliranim uslovima želudaÄnog soka omoguÄuje da se tokom imunoterapije mogu primenjivati veÄe doze alergoida nego nemodifikovanog ekstrakta polena pelina
Goodās syndrome with increasing Ī³Ī“ T-lymphocyte subpopulation: A case report
Introduction. Goodās syndrome is a rare cause of adultonset immunodeficiency
associated with thymoma. Goodās syndrome should be considered in patients
older than 40 years with the history of frequent infections. An abnormal
immunoglobulin profile needs further investigation and flow cytometry which
is crucial for establishing the diagnosis of Goodās syndrome. Case report. We
present a 56- year-old men with Goodās syndrome diagnosed after a twoyear
history of recurrent infections. Examination of immune status of the patient
showed decreased serum levels of all immunoglobulins. Flow cytometry of
peripheral blood lymphocyte revealed markedly reduced peripheral B cells, CD4
T-cell lymphopenia, inverted CD4/CD8 T-cell-ratio 0.37 (CD4 - 20.82%, CD8 -
70.7%). Analysis of the subpopulations of T-lymphocytes showed relative
increasing Ī³Ī“ T cell receptor (TCR) T lymphocytes. Computed tomography scan
of the chest showed a mediastinal mass compatible with thymoma of the
diameter of 40 mm. After initiation of intravenous immunoglobulins the
patient was in the good clinical condition and without bacterial
complications. As the patient refused the operative treatment we continued to
control the mediastinal tumor mass which did not increase during a 3-year
follow-up. Conclusion. The presented patient had a typical immunological
finding for Goodās syndrome, but also the increase in Ī³Ī“ TCR T-lymphocyte
subpopulation for which it is difficult to determine whether this is
pathogenetic or secondary reactive event
Artemisia vulgaris pollen allergoids digestibility in the simulated conditions of the gastrointestinal tract
Chemically modified allergens (allergoids) have found use in both traditional and novel forms of immunotherapy of allergic disorders. Novel forms of immunotherapy include local allergen delivery, via the gastrointestinal tract. This study conveys the gastrointestinal stability of three types ofmugwort pollen allergoids under simulated conditions of the gut. Allergoids of the pollen extract of Artemisia vulgaris were obtained by means of potassium cyanate, succinic and maleic anhydride. Gastrointestinal tract conditions (saliva, and gastric fluid) were simulated in accordance with the EU Pharmacopoeia. The biochemical and immunochemical properties of the derivatives following exposure to different conditions were monitored by determining the number of residual amino groups with 2,4,6-trinitrobenzenesulfonic acid, SDS PAGE, immunoblotting and inhibition of mugwort-specific IgE. Exposure to saliva fluid for 2 min did not influence the biochemical and immunochemical properties of the derivatives. In the very acidic conditions of the simulated gastric fluid, the degree of demaleylation and desuccinylation, even after 4 h exposure, was low, ranging from 10 to 30 %. The digestion patterns with pepsin proceeded rapidly in both the unmodified and modified samples. In all four cases, a highly resistant IgE-binding protein theMwof which was about 28 ā 35 kD, was present. Within the physiological conditions, no new IgE binding epitopes were revealed, as demonstrated by immunoblot and CAP inhibition of the mugwort specific IgE binding. An important conclusion of this study is the stability of the modified derivatives in the gastrointestinal tract of patients, within physiological conditions. The means that they are suitable for use inmuch higher concentrations in local forms of immunotherapy than unmodified ones