6 research outputs found
Structure and immunohistochemistry of the human lenticulostriate arteries
Background: Data about the structure and immunohistochemistry of the lenticulostriatearteries (LSAs), although very important for medical research and clinicalpractice, have been rarely reported in literature.Materials and methods: Fourty serially sectioned LSAs were stained with hematoxilinand eosin, and prepared for immunohistochemistry.Results: Our examination revealed a typical endothelial lining and a narrow subendothelialspace with subintimal smooth muscle cells occasionally. The internalelastic lamina was fragmented or absent in the smallest LSAs branches. The mediacoat, with a mean diameter of 148.5 Ī¼m, contained typical smooth muscle cellswhich formed 14.2 layers on average and showed a positive immune reactions foralfa-actin, desmine, laminin and collagen IV. The thin adventitial coat containedfibroblasts, collagen fibers, and nerve bundles, with the strongest immunopositivityto thyrosin hydroxilase. The immune reactions against CD31 and CD34 proteins,endothelial nitric oxide synthase, S 100 protein, neurofilament protein and synaptophysin,seem to be performed in the LSAs wall for the first time. Similarly,the thickness of the LSAs wall and its coats have never been reported, nor thenumber of the smooth muscle cell layers.Conclusions: Our results related to the structure and immunohistochemistry ofthe LSAs could be important in cerebrovascular pathology, neurology and neurosurgery
Age-related changes in the content of insulin: Like growth factor-l in rat brain
Although there has been extensive research on the effect of IGF-I on muscles and bone tissue, the effect on brain aging has received little attention. We investigated the IGF-I content in brains of differently aged rats. The IGF-I contents in cerebellar and cerebral cortex were found to be higher in immature rats (4-5 days old) compared to young adult (2.5 months old) and middle-aged (7.5-9 months old) rats. However, the decrease of mean IGF-I in middle-aged rats compared to immature animals was statistically significant only in the cerebellar codex. Our results indicate that IGF-I content decreases through the lifespan and maybe selectively in some brain regions.VrÅ”ena su istraživanja insulinu sliÄnog faktora rasta (IGF-I) na miÅ”iÄno i koÅ”tano tkivo, ali je posveÄena mala pažnja efektu na mozak u toku starenja. Mi smo ispitivali sadržaj IGF-I u moždanom tkivu pacova razliÄite starosti. NaÄeno je da su IGF-I koncentracije u kori malog mozga kao i velikog mozga mladih pacova (4-5 dana starih) viÅ”e u poreÄenju sa sadržajima grupe tek-odraslih pacova starosti 2,5 meseca i grupe neÅ”to starijih odraslih pacova (7,5-9 meseci starih). MeÄutim, smanjenje koncentracije IGF-I sadržaja samo u kori malog mozga neÅ”to starijih pacova (7,5-9 meseci) bilo je znaÄajno u odnosu na vrednosti u novoroÄenih (4-5 dana starih pacova). NaÅ”i rezultati ukazuju da IGF-I opada tokom života i moguÄe - selektivno u odreÄenim moždanim regionima.nul
Age-related changes in the content of insulin: Like growth factor-l in rat brain
Although there has been extensive research on the effect of IGF-I on muscles and bone tissue, the effect on brain aging has received little attention. We investigated the IGF-I content in brains of differently aged rats. The IGF-I contents in cerebellar and cerebral cortex were found to be higher in immature rats (4-5 days old) compared to young adult (2.5 months old) and middle-aged (7.5-9 months old) rats. However, the decrease of mean IGF-I in middle-aged rats compared to immature animals was statistically significant only in the cerebellar codex. Our results indicate that IGF-I content decreases through the lifespan and maybe selectively in some brain regions.VrÅ”ena su istraživanja insulinu sliÄnog faktora rasta (IGF-I) na miÅ”iÄno i koÅ”tano tkivo, ali je posveÄena mala pažnja efektu na mozak u toku starenja. Mi smo ispitivali sadržaj IGF-I u moždanom tkivu pacova razliÄite starosti. NaÄeno je da su IGF-I koncentracije u kori malog mozga kao i velikog mozga mladih pacova (4-5 dana starih) viÅ”e u poreÄenju sa sadržajima grupe tek-odraslih pacova starosti 2,5 meseca i grupe neÅ”to starijih odraslih pacova (7,5-9 meseci starih). MeÄutim, smanjenje koncentracije IGF-I sadržaja samo u kori malog mozga neÅ”to starijih pacova (7,5-9 meseci) bilo je znaÄajno u odnosu na vrednosti u novoroÄenih (4-5 dana starih pacova). NaÅ”i rezultati ukazuju da IGF-I opada tokom života i moguÄe - selektivno u odreÄenim moždanim regionima.nul
Expression of heat shock protein 70 (HSP70) in patients with colorectal adenocarcinoma - immunohistochemistry and Western blot analysis
The role of heat shock protein 70 (HSP70) expression has been investigated in various types of tumors. There are only little and controversial data about its clinical relevance in colorectal carcinoma, one of the most common carcinomas observed in humans. In this study we investigated expression of HSP70 in human colonic carcinoma and possible correlation with clinicopathology. To assess patterns (cytosolic and membrane) of HSP70 expression, the 48 surgically removed colorectal adenocarcinomas and 12 normal colonic and rectal mucosal samples were examined by immunohistochemistry and Western-blot. According to results of immunohistochemistry, expression of cytoplasmic HSP72 was significantly higher in colorectal carcinoma compared with normal and adjacent mucosa (p LT 0.01). In addition, there was significant increase in HSP72 expression in lymph node-positive compared to node-nevative group (p LT 0.001). Dukes C2 stage of colonic cancer showed significantly higher immunohistochemical score than Dukes B2 and B1 stage groups (p LT 0.05 i.e. p LT 0.02). There was no relation between expression of HSP72 and degree of tumor differentiation. Using Western blot analyses, we noticed elevated levels of cytosolic HSP70 in colorectal cancer cells compared to normal. Densitometric analysis of blots of plasma membrane HSP70 expression has shown decrease in colorectal cancer cells compared to normal mucosa. According to our results, overexpression of HSP72 in malignant tissues of patients with colorectal carcinoma is related to tumor progression, suggesting that these proteins could play an important role not only in tumorigenesis but also in the development of drug resistance. Further research is necessary to clarify the mechanisms responsible for differential HSP70 expression as well as its definitive role in colorectal cancer
Expression of heat shock protein 70 (HSP70) in patients with colorectal adenocarcinoma - immunohistochemistry and Western blot analysis
The role of heat shock protein 70 (HSP70) expression has been investigated in various types of tumors. There are only little and controversial data about its clinical relevance in colorectal carcinoma, one of the most common carcinomas observed in humans. In this study we investigated expression of HSP70 in human colonic carcinoma and possible correlation with clinicopathology. To assess patterns (cytosolic and membrane) of HSP70 expression, the 48 surgically removed colorectal adenocarcinomas and 12 normal colonic and rectal mucosal samples were examined by immunohistochemistry and Western-blot. According to results of immunohistochemistry, expression of cytoplasmic HSP72 was significantly higher in colorectal carcinoma compared with normal and adjacent mucosa (p LT 0.01). In addition, there was significant increase in HSP72 expression in lymph node-positive compared to node-nevative group (p LT 0.001). Dukes C2 stage of colonic cancer showed significantly higher immunohistochemical score than Dukes B2 and B1 stage groups (p LT 0.05 i.e. p LT 0.02). There was no relation between expression of HSP72 and degree of tumor differentiation. Using Western blot analyses, we noticed elevated levels of cytosolic HSP70 in colorectal cancer cells compared to normal. Densitometric analysis of blots of plasma membrane HSP70 expression has shown decrease in colorectal cancer cells compared to normal mucosa. According to our results, overexpression of HSP72 in malignant tissues of patients with colorectal carcinoma is related to tumor progression, suggesting that these proteins could play an important role not only in tumorigenesis but also in the development of drug resistance. Further research is necessary to clarify the mechanisms responsible for differential HSP70 expression as well as its definitive role in colorectal cancer
Age-related changes in the content of insulin: Like growth factor-l in rat brain
VrÅ”ena su istraživanja insulinu sliÄnog faktora rasta (IGF-I) na miÅ”iÄno i koÅ”tano tkivo, ali je posveÄena mala pažnja efektu na mozak u toku starenja. Mi smo ispitivali sadržaj IGF-I u moždanom tkivu pacova razliÄite starosti. NaÄeno je da su IGF-I koncentracije u kori malog mozga kao i velikog mozga mladih pacova (4-5 dana starih) viÅ”e u poreÄenju sa sadržajima grupe tek-odraslih pacova starosti 2,5 meseca i grupe neÅ”to starijih odraslih pacova (7,5-9 meseci starih). MeÄutim, smanjenje koncentracije IGF-I sadržaja samo u kori malog mozga neÅ”to starijih pacova (7,5-9 meseci) bilo je znaÄajno u odnosu na vrednosti u novoroÄenih (4-5 dana starih pacova). NaÅ”i rezultati ukazuju da IGF-I opada tokom života i moguÄe - selektivno u odreÄenim moždanim regionima.Although there has been extensive research on the effect of IGF-I on muscles and bone tissue, the effect on brain aging has received little attention. We investigated the IGF-I content in brains of differently aged rats. The IGF-I contents in cerebellar and cerebral cortex were found to be higher in immature rats (4-5 days old) compared to young adult (2.5 months old) and middle-aged (7.5-9 months old) rats. However, the decrease of mean IGF-I in middle-aged rats compared to immature animals was statistically significant only in the cerebellar codex. Our results indicate that IGF-I content decreases through the lifespan and maybe selectively in some brain regions