1,868 research outputs found

    Cultivating diversity and food quality. Proceedings of Diversifood EU Forum, Brussels, 11 April 2018

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    To tackle this issue, Diversifood team organised a forum with policy makers and stakeholders on the 11th of April 2018, in Brussels. Diversifood’s aim is to share results and key lessons including new approaches for the management of cultivated biodiversity, for plant breeding for sustainable farming systems, and new relationships among actors of food systems. In the afternoon, there was time for discussion, knowledge sharing, collecting feedback and extending current policies to include cultivating diversity and food quality (for FP9, CAP 2020, The outputs of this workshop will feed Diversifood’s final recommendations. The forum was kindly hosted by the European Committee of the Regions (Rue Belliard/Belliardstraat 101, 1040 Brussels)

    Characterization of the monocyte-specific esterase (MSE) gene

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    Carboxylic esterases are widely distributed in hematopoietic cells. Monocytes express the esterase isoenzyme (termed 'monocyte-specific esterase', MSE) that can be inhibited by NaF in the alpha-naphthyl acetate cytochemical staining. We examined the expression of MSE in normal cells and primary and cultured leukemia-lymphoma cells. The MSE protein was demonstrated by isoelectric focusing (IEF); MSE mRNA expression was investigated by Northern blotting and reverse transcriptase-polymerase chain reaction (RT-PCR). The following samples were positive for MSE protein and Northern mRNA expression: 20/24 monocytic, 4/32 myeloid, and 1/20 erythroid-megakaryocytic leukemia cell lines, but none of the 112 lymphoid leukemia or lymphoma cell lines; of the normal purified cell populations only the monocytes were positive whereas, T, B cells, and granulocytes were negative; of primary acute (myelo) monocytic leukemia cells (CD14-positive, FAB M4/M5 morphology) 14/20 were Northern mRNA and 11/14 IEF protein positive. RT-PCR revealed MSE expression in 29/49 Northern-negative lymphoid leukemia-lymphoma cell lines. The RT-PCR signals in monocytic cell lines were on average 50-fold stronger than the mostly weak trace expression in lymphoid specimens. On treatment with various biomodulators, only all-trans retinoic acid significantly upregulated MSE message and protein levels but could not induce new MSE expression in several leukemia cell lines; lipopolysaccharide and interferon-gamma increased MSE expression in normal monocytes. Analysis of DNA methylation with sensitive restriction enzymes showed no apparent regulation of gene expression by differential methylation; the MSE gene is evolutionarily conserved among mammalian species; the half-life of the human MSE transcripts was about 5-6 h. The extent of MSE expression varied greatly among different monocytic leukemia samples. However, the MSE overexpression in a significant number of specimens was not associated with gene amplification, gross structural rearrangements or point mutations within the cDNA region. Taken together, the results suggest that MSE expression is not absolutely specific for, but strongly associated with cells of the monocytic lineage; MSE is either not expressed at all or expressed at much lower levels in cells from other lineages. The biological significance, if any, of rare MSE messages in lymphoid cells detectable only by the hypersensitive RT-PCR remains unclear. Further studies on the regulation of this gene and on the physiological function of the enzyme will no doubt be informative with respect to its striking overexpression in some malignant cells and to a possible role in the pathobiology of monocytic leukemias

    High intensity study of THz detectors based on field effect transistors

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    Terahertz power dependence of the photoresponse of field effect transistors, operating at frequencies from 0.1 to 3 THz for incident radiation power density up to 100 kW/cm^2 was studied for Si metal-oxide-semiconductor field-effect transistors and InGaAs high electron mobility transistors. The photoresponse increased linearly with increasing radiation power up to kW/cm^2 range. The saturation of the photoresponse was observed for all investigated field effect transistors for intensities above several kW/cm^2. The observed signal saturation is explained by drain photocurrent saturation similar to saturation in direct currents output characteristics. The theoretical model of terahertz field effect transistor photoresponse at high intensity was developed. The model explains quantitatively experimental data both in linear and nonlinear (saturation) range. Our results show that dynamic range of field effect transistors is very high and can extend over more than six orderd of magnitudes of power densities (from 0.5 mW/cm^2 to 5 kW/cm^2)

    How to make a 350-m-thick lowstand systems tract in 17,000 years: The Late Pleistocene Po River (Italy) lowstand wedge

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    The 350-m-thick succession of the Po River lowstand wedge (Italy) associated with the Last Glacial Maximum (deposited over ~17 k.y) contains stratal architecture at a physical scale commonly attributed to much longer time scales, with complex, systematically varying internal clinothem characteristics. This study investigated clinothem stacking patterns and controls through the integration of seismic reflection data with sediment attributes, micropaleontology, regional climate, eustacy, and high-resolution age control possible only in Quaternary sequences. Three clinothem types are differentiated based on topset geometry, shelf-edge and onlap-point trajectory, internal seismic facies, and interpreted bottomset deposits: type A has moderate topset aggradation, ascending shelf-edge trajectory, and mass-transport bottomset deposits; type B has eroded topset, descending shelf-edge trajectory, and bottomset distributary channel-lobe complexes; and type C has maximal topset aggradation, ascending shelf-edge trajectory, and concordant bottomsets. Type A and C clinothems exhibit reduced sediment bypass and delivery to the basin, whereas type B clinothems are associated with short intervals of increased sediment export from the shelf to deeper water. Clinothems individually span a range of 0.4–4.7 k.y., contemporaneous with significant eustatic and climate changes, but their stacking patterns resemble those found in ancient successions and ascribed to significantly longer durations, indicating that (1) the response time of ancient continental margin–scale systems to high-frequency variations in accommodation and sediment supply could be as short as centuries, (2) even millennial- to centennial-scale stratal units can record substantial influence of allogenic controls, and (3) sandy deposits can be compartmentalized even in a short-duration lowstand systems tract

    Recent Decisions

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    Comments on recent decisions by William C. Rindone, Ray F. Drexler, Eugene G. Griffin, Ronald Patrick Smith, and John G. Curran

    Ionic conductivity in Li2O-Al2O3-SiO2 based glasses and glass ceramics

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    The complex conductivity of lithium aluminosilicate based glasses and glass-ceramics (Zerodur from Schott) has been investigated in a broad range of temperatures (200 K &lt; T &lt; 700 K) and frequencies (10 mHz&lt;v&lt;2.5 THz). The data are presented in terms of the conductivity and the electrical modulus formalisms. The width of the modulus loss peak as measured for the ceramic sample is broader than that determined for its precursor glass. This result is shown to be associated with the considerably smaller dc conductivity of this material.</jats:p
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