Calmodulin-like proteins localized to the conoid regulate motility and cell invasion by Toxoplasma gondii

Toxoplasma gondii contains an expanded number of calmodulin (CaM)-like proteins whose functions are poorly understood. Using a combination of CRISPR/Cas9-mediated gene editing and a plant-like auxin-induced degron (AID) system, we examined the roles of three apically localized CaMs. CaM1 and CaM2 were individually dispensable, but loss of both resulted in a synthetic lethal phenotype. CaM3 was refractory to deletion, suggesting it is essential. Consistent with this prediction auxin-induced degradation of CaM3 blocked growth. Phenotypic analysis revealed that all three CaMs contribute to parasite motility, invasion, and egress from host cells, and that they act downstream of microneme and rhoptry secretion. Super-resolution microscopy localized all three CaMs to the conoid where they overlap with myosin H (MyoH), a motor protein that is required for invasion. Biotinylation using BirA fusions with the CaMs labeled a number of apical proteins including MyoH and its light chain MLC7, suggesting they may interact. Consistent with this hypothesis, disruption of MyoH led to degradation of CaM3, or redistribution of CaM1 and CaM2. Collectively, our findings suggest these CaMs may interact with MyoH to control motility and cell invasion

Regime of the Filchner-Ronne Ice Shelves, Antarctica

Seismic, radio-echo, TWERLE balloon and oceanographic data of the Filchner-Ronne ice shelves are analysed. A large thin area in the central Ronne Ice Shelf is found to differ from the morphology of the Ross and Filchner ice shelves. This area is partly filled by basal saline ice, which can be attributed to regional regelation caused by oceanic circulation

Miocene isotope zones, paleotemperatures, and carbon maxima events at intermediate water-depth, Site 593, Southwest Pacific

Oxygen and carbon isotopic stratigraphies are presented from both benthic and planktic foraminifera for the late early Miocene to earliest Pliocene interval (c. 19–5 Ma) of intermediate water-depth DSDP Site 593 in the southern Tasman Sea. The benthic values are interpreted as recording Miocene Southern Component Intermediate Water, while the planktic species record the Miocene mode and surface water signals. Comparisons are made between temperate Site 593 and the intermediate-depth polar Site 747 in the southern Indian Ocean. Glacial Mi zones Mi1b–Mi6, representing extreme glacial events, are evident in both the Site 593 intermediate and surface water records. Miocene Southern Component Intermediate Water δ18O values are generally lighter than the Holocene equivalent (Antarctic Intermediate Water), indicating slightly warmer intermediate waters and/or less global ice volume. The benthic-planktic gradient is interpreted as indicating a less stratified Tasman Sea during the Miocene. The benthic δ13C record contains most of the global carbon maxima (CM) events, CM1–7 (CM1–6 = the Monterey Excursion). Like global deep-water records, the Tasman Sea intermediate water δ13C values indicate that most CM events correspond with Mi glacials, including Mi4 at Site 593, not reported previously. Intermediate waters play an important role in propagating climatic changes from the polar regions to the tropics, and the Site 593 dataset provides a full water column record of the structure of Miocene intermediate to surface watermasses prior to the modern situation