Dothistroma septosporum: producción de esporas y condiciones climáticas

Dothistroma septosporum, the causal agent of Dothistroma needle blight is a widespread fungus which infects more than 80 species of coniferous trees through the entire world. Spreading of the infection is strongly affected by climatic factors of each locality where it is recorded. We attempt to describe the concrete limiting climatic factors necessary for the releasing of conidia of D. septosporum and to find out the timing of its spore production within the year. For this purpose we used an automatic volumetric spore trap and an automatic meteorological station. We found that a minimum daily average temperature of 10 °C was necessary for any spore production, as well as a long period of high air humidity. The values obtained in the present study were a little bit higher than those previously published, which may arise questions about a possible changing trend of the behaviour in the development of the Dothistroma needle blight causal agent. We used autoregressive integrated moving average (ARIMA) models to predict the spore counts on the base of previous values of spore counts and dew point. For a locality from Hackerovka, the best ARIMA model was 1,0,0; and for a locality from Lanzhot, the best was 3,1,0.El Dothistroma septosporum, el agente causal del tizón Dothistroma de las acículas, es un hongo ampliamente distribuido que infecta más de 80 especies de coníferas en el mundo. La propagación de la infección está fuertemente afectada por factores climáticos de cada localidad donde se registra. Tratamos de describir los factores limitantes necesarios para la liberación de los conidios de D. septosporum y averiguar el momento de la producción de esporas en el año. Para este fin se utilizó una trampa de esporas volumétrica y una estación meteorológica automáticas. Se ha encontrado que fue necesaria una temperatura media mínima diaria de 10 °C para cualquier producción de esporas, así como un largo período de alta humedad del aire. Los valores obtenidos en el presente estudio fueron un poco más altos que los publicados anteriormente, que pueden surgerir preguntas acerca de una posible tendencia cambiante de la conducta en el desarrollo del agente causal del tizón Dothistroma de las acículas. Se utilizaron modelos autorregresivos integrados media móvil (ARIMA) para predecir los conteos de esporas sobre la base de los valores anteriores de los recuentos de esporas y del punto de rocío. Para una localidad de Hackerovka, el mejor modelo ARIMA es 1.0.0 y para una localidad de Lanzhot, el mejor fue 3.1.0

Disciplinary Segregation’s Effects on Inmate Behavior: Institutional and Community Outcomes

Disciplinary segregation (DS) is practiced in a variety of correctional settings and a growing body of research explores its subsequent effects among offenders. The present study contributes to this literature by analyzing the impact of short-term DS on violent infractions and community recidivism among a sample of inmates in Washington State. We assessed the impact of DS on these outcomes from deterrence and stain theory perspectives while controlling for social support variables such as visitations and correctional programming. Mentally ill offenders were excluded, as their abilities to make rational choices may be inconsistent with deterrence theory. Results show DS does not significantly affect post-DS infractions. Social supports significantly reduced inmates’ odds of violent infractions while incarcerated. Community models indicate no substantive differences between the DS and non-DS groups on post-prison convictions 3 years after release. Overall, DS exhibited limited effects on offenders’ institutional or community outcomes

Understanding Offender Needs Over Forms of Isolation Using a Repeated Measures Design

A number of studies find that solitary confinement is associated with mental impairment. Yet, confinement dosage and which individual and exogenous variables lead to mental impairment have received less attention. This study of 2 years of data on disciplinary segregation male inmates employs a repeated measures design to examine how isolation affects mental health and psychological needs. The findings indicate that the duration of disciplinary segregation and incarceration, incidence of homelessness, and other individual-level factors had deleterious effects on mental health and psychological needs. Vocational programming and a high school education were found to be protective factors for psychological needs

NADK-mediated de novo NADP(H) synthesis is a metabolic adaptation essential for breast cancer metastasis

Metabolic reprogramming and metabolic plasticity allow cancer cells to fine-tune their metabolism and adapt to the ever-changing environments of the metastatic cascade, for which lipid metabolism and oxidative stress are of particular importance. NADPH is a central co-factor for both lipid and redox homeostasis, suggesting that cancer cells may require larger pools of NADPH to efficiently metastasize. NADPH is recycled through reduction of NADP+ by several enzymatic systems in cells; however, de novo NADP+ is synthesized only through one known enzymatic reaction, catalyzed by NAD+ kinase (NADK). Here, we show that NADK is upregulated in metastatic breast cancer cells enabling de novo production of NADP(H) and the expansion of the NADP(H) pools thereby increasing the ability of these cells to adapt to the challenges of the metastatic cascade and efficiently metastasize. Mechanistically, we found that metastatic signals lead to a histone H3.3 variant-mediated epigenetic regulation of the NADK promoter, resulting in increased NADK levels in cells with metastatic ability. Together, our work presents a previously uncharacterized role for NADK and de novo NADP(H) production as a contributor to breast cancer progression and suggests that NADK constitutes an important and much needed therapeutic target for metastatic breast cancers