Distribution of Free Marine Viruses of Lower Chesapeake Bay and Their Effects on Life-History Parameters of the Estuarine Copepod \u3ci\u3eAcartia tonsa\u3c/i\u3e Dana

Naturally occurring viruses are very abundant in fresh, estuarine, and marine waters, with densities on the order of 105-108 viruses ml-1. Research has focused on virus effects on bacteria, cyanobacteria, and phytoplankton, as well as mechanisms of virus production and decay. However, little is known about the distribution of viruses in benthic environments or virus effects on organisms in higher trophic levels. To determine the distribution of virus-like particles (VLPs) and bacteria in the lower Chesapeake Bay, vertical profiles of VLPs and bacteria were determined through the water column and 15-25 cm into the sediment at five stations. VLPs were about ten times more abundant in sediment pore water (3.7 x 108 VLPs ml-1) than in the water column (2.4 x 106 bacteria ml-1). In the water column, VLP and bacteria counts exhibited significant differences among stations, with highest values on the southern side of the Bay mouth. In the sediment pore water, VLP abundance varied with depth and was negatively correlated with grain size. Bacteria abundance was highest at the sediment-water-interface, decreased in the first cm of sediment, was uniform in the deeper horizons, and showed no significant relationship with grain size. These are the first data indicating the abundance of VLPs below the surface layer of sediment in aquatic systems and demonstrate that VLPs are components of the sediment microbial community. To evaluate virus effects on zooplankton, concentrated VLPs were added to cultures of Acartia tonsa, then egg production, egg hatching success, and mortality of copepods were measured. Elevated VLP concentrations were obtained by concentrating the virus-size fraction of fresh seawater or water from copepod cultures. Across six experiments, no detrimental effects of viruses on copepods were demonstrated. Similarly, adding pulverized copepods and copepod exudates to water containing healthy copepods yielded no measurable detrimental effect. Therefore, there was no support for the hypothesis that viruses infect and negatively affect the longevity and fecundity of A. tonsa

Four-way regulation of mosquito yolk protein precursor genes by juvenile hormone-, ecdysone-, nutrient-, and insulin-like peptide signaling pathways.

Anautogenous mosquito females require a meal of vertebrate blood in order to initiate the production of yolk protein precursors by the fat body. Yolk protein precursor gene expression is tightly repressed in a state-of-arrest before blood meal-related signals activate it and expression levels rise rapidly. The best understood example of yolk protein precursor gene regulation is the vitellogenin-A gene (vg) of the yellow fever mosquito Aedes aegypti. Vg-A is regulated by (1) juvenile hormone signaling, (2) the ecdysone-signaling cascade, (3) the nutrient sensitive target-of-rapamycin signaling pathway, and (4) the insulin-like peptide (ILP) signaling pathway. A plethora of new studies have refined our understanding of the regulation of yolk protein precursor genes since the last review on this topic in 2005 (Attardo et al., 2005). This review summarizes the role of these four signaling pathways in the regulation of vg-A and focuses upon new findings regarding the interplay between them on an organismal level

Do Viruses Affect Fecundity and Survival of the Copepod Acartia tonsa Dana?

Naturally occurring viruses are extremely abundant in aquatic systems, and they infect bacteria, cyanobacteria, prokaryotic and eukaryotic phytoplankton, heterotrophic nanoflagellates, fish and mammals. Viral infections of single-celled organisms have been studied intensively in the past decade, but little is known about the effects of viruses on aquatic metazoans, other than for some economically important species. Because zooplankton assemblages are often dominated in number and biomass by copepods, we used them as model organisms to study the effects of naturally occurring viruses on higher trophic levels. We attempted to induce viral infection in laboratory-reared cultures of the estuarine copepod Acartia tonsa Dana by exposing them to elevated concentrations of natural viruses in seawater. We found no negative effects of such exposure on copepod fecundity, larval survival or adult survival

Effects of Epiphyte Load on Optical Properties and Photosynthetic Potential of the Seagrasses Thalassia Testudinum Banks ex König and Zostera Marina L

The biomass and optical properties of seagrass leaf epiphytes were measured to evaluate their potential impact on the photosynthetic performance of the seagrasses Thalassia testudinum Banks ex König (turtlegrass) and Zostera marina L. (eelgrass). Turtlegrass was obtained from oligotrophic waters near Lee Stocking Island, Bahamas; eelgrass was collected from a eutrophic environment in Monterey Bay, California. Leaf–epiphyte loads were characterized visually and quantified using measurements of their phospholipid biomass. Light absorption and reflectance of the intact epiphyte layer were determined spectrophotometrically. Turtlegrass epiphytes from the oligotrophic site absorbed a maximum of 36% of incident light in peak chlorophyll absorption bands, whereas higher epiphyte loads on eelgrass from the more eutrophic Monterey Bay absorbed 60% of incident light in peak chlorophyll absorption bands. The combination of intact epiphyte–leaf complexes and spectral measurements enabled us to construct a quantitative relationship between epiphyte biomass and light attenuation, and, by extension, between epiphyte bio- mass and seagrass photosynthesis. The model yielded a robust, positive relationship between epiphyte biomass and the absorption of photons in photosynthetically important wavelengths, and it generated a strong negative relationship between epiphyte biomass and spectral photosynthesis of their seagrass hosts. Furthermore, the calculations of photosynthesis highlighted the significant differences between PAR and spectral models of photosynthesis, illustrating that the spectral quality of the incident flux must be considered when evaluating the effects of epiphyte load on seagrass leaf photosynthesis. Verification of the model—using direct measurements of photosynthesis and a variety of epiphyte and macrophyte combinations from different locations--is warranted

Allele-specific expression and eQTL analysis in mouse adipose tissue.

BackgroundThe simplest definition of cis-eQTLs versus trans, refers to genetic variants that affect expression in an allele specific manner, with implications on underlying mechanism. Yet, due to technical limitations of expression microarrays, the vast majority of eQTL studies performed in the last decade used a genomic distance based definition as a surrogate for cis, therefore exploring local rather than cis-eQTLs.ResultsIn this study we use RNAseq to explore allele specific expression (ASE) in adipose tissue of male and female F1 mice, produced from reciprocal crosses of C57BL/6J and DBA/2J strains. Comparison of the identified cis-eQTLs, to local-eQTLs, that were obtained from adipose tissue expression in two previous population based studies in our laboratory, yields poor overlap between the two mapping approaches, while both local-eQTL studies show highly concordant results. Specifically, local-eQTL studies show ~60% overlap between themselves, while only 15-20% of local-eQTLs are identified as cis by ASE, and less than 50% of ASE genes are recovered in local-eQTL studies. Utilizing recently published ENCODE data, we also find that ASE genes show significant bias for SNPs prevalence in DNase I hypersensitive sites that is ASE direction specific.ConclusionsWe suggest a new approach to analysis of allele specific expression that is more sensitive and accurate than the commonly used fisher or chi-square statistics. Our analysis indicates that technical differences between the cis and local-eQTL approaches, such as differences in genomic background or sex specificity, account for relatively small fraction of the discrepancy. Therefore, we suggest that the differences between two eQTL mapping approaches may facilitate sorting of SNP-eQTL interactions into true cis and trans, and that a considerable portion of local-eQTL may actually represent trans interactions

Observations of Extrasolar Planets During the non-Cryogenic Spitzer Space Telescope Mission

Precision infrared photometry from Spitzer has enabled the first direct studies of light from extrasolar planets, via observations at secondary eclipse in transiting systems. Current Spitzer results include the first longitudinal temperature map of an extrasolar planet, and the first spectra of their atmospheres. Spitzer has also measured a temperature and precise radius for the first transiting Neptune-sized exoplanet, and is beginning to make precise transit timing measurements to infer the existence of unseen low mass planets. The lack of stellar limb darkening in the infrared facilitates precise radius and transit timing measurements of transiting planets. Warm Spitzer will be capable of a precise radius measurement for Earth-sized planets transiting nearby M-dwarfs, thereby constraining their bulk composition. It will continue to measure thermal emission at secondary eclipse for transiting hot Jupiters, and be able to distinguish between planets having broad band emission versus absorption spectra. It will also be able to measure the orbital phase variation of thermal emission for close-in planets, even non-transiting planets, and these measurements will be of special interest for planets in eccentric orbits. Warm Spitzer will be a significant complement to Kepler, particularly as regards transit timing in the Kepler field. In addition to studying close-in planets, Warm Spitzer will have significant application in sensitive imaging searches for young planets at relatively large angular separations from their parent stars.Comment: 12 pages, 7 figures, to appear in "Science Opportunities for the Warm Spitzer Mission

Vertical Profiles of Virus-Like Particles and Bacteria in the Water Column and Sediments of Chesapeake Bay, USA

Vertical profiles of virus-like particles (VLPs) and bacteria were determined by near-synoptic sampling through the water column and 15 to 25 cm into the sediment at 5 stations across the mouth of Chesapeake Bay, USA. VLPs were about 10 times more abundant in the pore water (grand mean = 3.6 x 10(8) VLPs ml(-1)) than in the water column (grand mean = 3.8 x 10(7) VLPs ml(-1)). Similarly, bacteria counts were about 3 times higher in the pore water (grand mean = 6.5 x 10(6) bacteria ml(-1)) than in the water column (grand mean = 2.4 x 10(6) bacteria ml(-1)). The virus to bacteria ratio (VBR) was greater in the pore water (range = 29 to 85) than in the water column (range = 12 to 17). The VER was lowest in the water-over-boxcore samples and variable in the pore water. Counts of VLPs and bacteria were positively correlated in the water column, although neither was correlated to chlorophyll a. In the water column, VLPs and bacteria counts exhibited significant differences among stations, with the highest values on the southern side of the Bay mouth. In the pore water, VLP abundance varied with depth and was negatively correlated to grain size. Bacteria abundance was highest at the sediment-water interface, decreased in the first cm of sediment, was uniform in the deeper horizons, and showed no significant relationship with grain size. Bacteria counts in pore water were not significantly different among stations. In contrast, VLP abundances in pore water were significantly different among stations, although they did not increase in abundance from north to south across the Bay mouth. as did counts of water-column VLPs. These are the first data indicating the abundance of VLPs below the surface layer of sediment in aquatic systems and demonstrate that VLPs are components of the sedimentary microbial community to at least 25 cm depth

Potential Export of Unattached Benthic Macroalgae to the Deep Sea Through Wind Driven Langmuir Circulation

Carbon export to the deep sea is conventionally attributed to the sinking of open ocean phytoplankton. Here, we report a Langmuir supercell event driven by high winds across the shallow Great Bahama Bank that organized benthic non-attached macroalgae, Colpomenia sp., into visible windrows on the seafloor. Ocean color satellite imagery obtained before and after the windrows revealed a 588 km2 patch that rapidly shifted from highly productive macroalgae to bare sand. We assess a number of possible fates for this macroalgae and contend that this event potentially transported negatively buoyant macroalgae to the deep Tongue of the Ocean in a pulsed export of \u3e 7 x 1010 g of carbon. This is equivalent to the daily carbon flux of phytoplankton biomass in the pelagic tropical North Atlantic and 0.2-0.8% of daily carbon flux from the global ocean. Coastal banks and bays are highly productive ecosystems that may contribute substantially to carbon export to the deep sea. Citation: Dierssen, H. M., R. C. Zimmerman, L. A. Drake, and D. J. Burdige (2009), Potential export of unattached benthic macroalgae to the deep sea through wind-driven Langmuir circulation, Geophys. Res. Lett., 36, L04602, doi: 10.1029/2008GL036188

Benthic Ecology From Space: Optics and Net Primary Production in Seagrass and Benthic Algae Across the Great Bahama Bank

Development of repeatable and quantitative tools are necessary for determining the abundance and distribution of different types of benthic habitats, detecting changes to these ecosystems, and determining their role in the global carbon cycle. Here we used ocean color remote sensing techniques to map different major groups of primary producers and estimate net primary productivity (NPP) across Great Bahama Bank (GBB). Field investigations on the northern portion of the GBB in 2004 revealed 3 dominant types of benthic primary producers: seagrass, benthic macroalgae, and microalgae attached to sediment. Laboratory measurements of NPP ranged from barely net autotrophic for grapestone sediment with thin microalgal biofilm to highly productive for dense accumulations of brown macroalgae. A logarithmic relationship between NPP and green seafloor reflectance described the general trend in NPP across various benthic constituents. Using a radiative transfer-based approach, satellite-derived estimates of NPP for the region totaled similar to ~2 x 1013 gC yrˉ¹ across the GBB. The prevailing benthic habitat was mapped as sediment with little to no microalgal biofilm. Moderate to dense seagrass meadows of Thalassia testudinumwere the dominant primary producers and contributed over 80% of NPP in the region. If the vast majority of seagrass leaves decompose in the primarily carbonate sediments, carbonate dissolution processes associated with this decomposition may result in sequestration of seagrass above- and below-ground carbon into the bicarbonate pool (2.4 x 1013 gC yrˉ¹), where it has a residence time on the order of tens of thousands of years