A derivative of Zymomonas mobilis ATCC 10988 with impared ethanol production

A derivative of Zymomonas mobilis ATCC 10988 has been isolated from cells treated with acridine orange. This derived strain, designated CU1, was found to have markedly decreased ethanol production and concomitant glucose utilisation capabilities when grown on high concentrations of glucose. Additionally, it was found that CU1 had altered alcohol dehydrogenase activity and also lacks at least one of the natural plasmids of Z.mobilis, the 3kb species. © 1984 Science and Technology Letters

Growth requirements and the establishment of a chemically defined minimal medium in Zymomonasmobilis

A chemically defined minimal medium which fulfils the growth requirements of different Zymomonasmobilis strains has been established. The kinetics of ethanol production of the strains ATCC 10988, CU1, CP4 and 11163 grown on the minimal medium at different glucose concentrations were measured. All strains produced ethanol at rates similar to those on complete medium. The minimal medium described is suitable to study spontaneous metabolic deficiciencies and regulation of enzyme activities in Z.mobilis. © 1985 Kluwer Academic Publishers

Glutamine uptake in Zymomonas mobilis

Glutamine was transported in Zymomonas mobilis by a mechanism following Michaelis-Menten kinetics with a Km value for glutamine 8 x 10-5 M and a Vmax value of 15.4 nmol.mg-1, min-1 or 40 nmol.mg-1.min-1 for cells growing on complete medium or minimal medium respectively. The transport of glutamine was energy-dependent and more or less specific for glutamine when cell were grown on rich media. Evidence provided via spheroplasts suggests the possible involvement of a periplasmic component in this transport system. © 1990 Kluwer Academic Publishers

Bioconversion of fruit and sugar beet extracts by Zymomonas mobilis to ethanol and other fine chemicals

Zymomonas mobilis can grow and produce ethanol on extracts of apples, oranges, peaches, watermelons, and sugar beets without the addition of any nutrients. The best substrate for growth was watermelon extract. Strain CP4 showed the best growth kinetics on all the extracts tested, but strain NCIB 11163 failed to grow on sugar beet extracts. The highest ethanol production and yield was obtained by strain ATCC 10988 when grown on apple, orange, and watermelon extracts. Isopentanol, acetic acid, and low amounts of histidine were the major by-products produced. Traces of formaldehyde, methanol, phenol, and other higher alcohols were also detected, but H2S was not detectable. © 1989