Exploring Attitudes of Future Educators About Sexual Minority Youth

Fifty-two secondary teacher candidates from a Canadian university completed questionnaires assessing levels of homoprejudice, knowledge of homosexuality, and perceptions of professional issues related to sexual minority youth. The level of homoprejudice in this sample was lower than in earlier studies with teachers, and lower homoprejudice was found to be significantly related to higher professional commitment and willingness to assist sexual minority students. As well greater knowledge of homosexual issues was significantly related to lower levels of homoprejudice, and men had significantly more homoprejudiced attitudes than women.Cinquante-deux stagiaires d’une université canadienne ont complété des questionnaires évaluant les préjugés contre l’homosexualité, les connaissances sur l’homosexualité et les perceptions des enjeux professionnels liés aux jeunes appartenant à une minorité sexuelle. Cet échantillon a démontré moins de préjugés face à l’homosexualité que ceux des études précédentes impliquant des enseignants; or, un rapport significatif existe entre des niveaux peu élevés de préjugés face à l’homosexualité d’une part, et un plus grand engagement des les jeunes membres d’une minorité sexuelle et une volonté accrue de leur venir en aide d’autre part. Un lien significatif existe également entre des connaissances plus importantes relatives aux enjeux touchant les homosexuels et des niveaux moins élevés de préjugés face à l’homosexualité. Finalement, les hommes ont démontré, de manière significative, plus d’attitudes homophobes que les femmes

Acute-Phase Serum Amyloid A: An Inflammatory Adipokine and Potential Link between Obesity and Its Metabolic Complications

BACKGROUND: Obesity is associated with low-grade chronic inflammation, and serum markers of inflammation are independent risk factors for cardiovascular disease (CVD). However, the molecular and cellular mechanisms that link obesity to chronic inflammation and CVD are poorly understood. METHODS AND FINDINGS: Acute-phase serum amyloid A (A-SAA) mRNA levels, and A-SAA adipose secretion and serum levels were measured in obese and nonobese individuals, obese participants who underwent weight-loss, and persons treated with the insulin sensitizer rosiglitazone. Inflammation-eliciting activity of A-SAA was investigated in human adipose stromal vascular cells, coronary vascular endothelial cells and a murine monocyte cell line. We demonstrate that A-SAA was highly and selectively expressed in human adipocytes. Moreover, A-SAA mRNA levels and A-SAA secretion from adipose tissue were significantly correlated with body mass index ( r = 0.47; p = 0.028 and r = 0.80; p = 0.0002, respectively). Serum A-SAA levels decreased significantly after weight loss in obese participants ( p = 0.006), as well as in those treated with rosiglitazone ( p = 0.033). The magnitude of the improvement in insulin sensitivity after weight loss was significantly correlated with decreases in serum A-SAA ( r = −0.74; p = 0.034). SAA treatment of vascular endothelial cells and monocytes markedly increased the production of inflammatory cytokines, e.g., interleukin (IL)-6, IL-8, tumor necrosis factor alpha, and monocyte chemoattractant protein-1. In addition, SAA increased basal lipolysis in adipose tissue culture by 47%. CONCLUSIONS: A-SAA is a proinflammatory and lipolytic adipokine in humans. The increased expression of A-SAA by adipocytes in obesity suggests that it may play a critical role in local and systemic inflammation and free fatty acid production and could be a direct link between obesity and its comorbidities, such as insulin resistance and atherosclerosis. Accordingly, improvements in systemic inflammation and insulin resistance with weight loss and rosiglitazone therapy may in part be mediated by decreases in adipocyte A-SAA production

MRI-guided prostate radiation therapy planning: Investigation of dosimetric accuracy of MRI-based dose planning

Background and purpose: Dose planning requires a CT scan which provides the electron density distribution for dose calculation. MR provides superior soft tissue contrast compared to CT and the use of MR-alone for prostate planning would provide further benefits such as lower cost to the patient. This study compares the accuracy of MR-alone based dose calculations with bulk electron density assignment to CT-based dose calculations for prostate radiotherapy

Organoid cultures derived from patients with advanced prostate cancer

The lack of in vitro prostate cancer models that recapitulate the diversity of human prostate cancer has hampered progress in understanding disease pathogenesis and therapy response. Using a 3D organoid system, we report success in long-term culture of prostate cancer from biopsy specimens and circulating tumor cells. The first seven fully characterized organoid lines recapitulate the molecular diversity of prostate cancer subtypes, including TMPRSS2-ERG fusion, SPOP mutation, SPINK1 overexpression, and CHD1 loss. Whole-exome sequencing shows a low mutational burden, consistent with genomics studies, but with mutations in FOXA1 and PIK3R1, as well as in DNA repair and chromatin modifier pathways that have been reported in advanced disease. Loss of p53 and RB tumor suppressor pathway function are the most common feature shared across the organoid lines. The methodology described here should enable the generation of a large repertoire of patient-derived prostate cancer lines amenable to genetic and pharmacologic studies