Microfluidics: reframing biological enquiry

The underlying physical properties of microfluidic tools have led to new biological insights through the development of microsystems that can manipulate, mimic and measure biology at a resolution that has not been possible with macroscale tools. Microsystems readily handle sub-microlitre volumes, precisely route predictable laminar fluid flows and match both perturbations and measurements to the length scales and timescales of biological systems. The advent of fabrication techniques that do not require highly specialized engineering facilities is fuelling the broad dissemination of microfluidic systems and their adaptation to specific biological questions. We describe how our understanding of molecular and cell biology is being and will continue to be advanced by precision microfluidic approaches and posit that microfluidic tools - in conjunction with advanced imaging, bioinformatics and molecular biology approaches - will transform biology into a precision science

Characterization and comparative analyses of zebrafish intelectins: Highly conserved sequences, diversified structures and functions

Intelectin family, also called the X-lectin family, is a newly discovered gene family involved in development and innate immunity. However, no research was carried out for this gene family in the model organism zebrafish. Here we present the first characterization of seven zebrafish intelectins (zINTLs) and the first systematic comparative analysis of intelectins from various species in order to provide some clues to the function and evolution of this gene family. We examined the expression patterns of zINTLs in various development stages, normal adults, and Aeromonas salmonicida infected adults. Results showed that zINTL1-3 were highly expressed in one or several adult tissues. zINTL4-7, however, were expressed at quite low levels both in adults and various development stages, and some of them showed relaxation of functional constrains as revealed by Ka/Ks calculation. Of the seven zINTLs, zINTL3 was expressed predominantly in the liver and highly up-regulated upon infection, suggesting its important roles in immunity. Based on the characterization of zebrafish intelectins, we then conducted a systematic survey of intelectin members in various species and made comparative analyses. We found out that intelectin family may be a deuterostome specific gene family; and their expression patterns, quaternary structures and glycosylations vary considerably among various species, though their sequences are highly conserved. Moreover, these varied features have evolved multiple times independently in different species, resulting in species-specific protein structures and expression patterns. © 2008 Elsevier Ltd. All rights reserved.link_to_subscribed_fulltex