Thioredoxin reductase enzyme activity measurements showed as nmolDTNB reduced per min per mg protein

<p><b>Copyright information:</b></p><p>Taken from "Thioredoxin reductase is a key factor in the oxidative stress response of WCFS1"</p><p>http://www.microbialcellfactories.com/content/6/1/29</p><p>Microbial Cell Factories 2007;6():29-29.</p><p>Published online 28 Aug 2007</p><p>PMCID:PMC2174512.</p><p></p> The strain NZ7601 was grown (black bars) aerobically and (gray bars) anaerobically at 37°C and was induced with 0 or 50 ng/ml nisinZ. After 5 hours of induction cell free extracts were prepared and TR activity was measured. The data shown above is the result of three independent experiments

Nonhierarchical Flux Regulation Exposes the Fitness Burden Associated with Lactate Production in <i>Synechocystis</i> sp. PCC6803

Cyanobacteria are mostly engineered to be sustainable cell-factories by genetic manipulations alone. Here, by modulating the concentration of allosteric effectors, we focus on increasing product formation without further burdening the cells with increased expression of enzymes. Resorting to a novel 96-well microplate cultivation system for cyanobacteria, and using lactate-producing strains of <i>Synechocystis</i> PCC6803 expressing different l-lactate dehydrogenases (LDH), we titrated the effect of 2,5-anhydro-mannitol supplementation. The latter acts in cells as a nonmetabolizable analogue of fructose 1,6-bisphosphate, a known allosteric regulator of one of the tested LDHs. In this strain (SAA023), we achieved over 2-fold increase of lactate productivity. Furthermore, we observed that as carbon is increasingly deviated during growth toward product formation, there is an increased fixation rate in the population of spontaneous mutants harboring an impaired production pathway. This is a challenge in the development of green cell factories, which may be countered by the incorporation in biotechnological processes of strategies such as the one pioneered here

Weblogo representation of conserved promotor regions in peroxide affected genes found using bioinformatics tools A) Regulatory motif -and B) uncharacterized regulatory motif

<p><b>Copyright information:</b></p><p>Taken from "Thioredoxin reductase is a key factor in the oxidative stress response of WCFS1"</p><p>http://www.microbialcellfactories.com/content/6/1/29</p><p>Microbial Cell Factories 2007;6():29-29.</p><p>Published online 28 Aug 2007</p><p>PMCID:PMC2174512.</p><p></p

Additional file 3: of Molecular assessment of bacterial vaginosis by Lactobacillus abundance and species diversity

Country of origin of 20 women with BV and 20 women without BV. (PDF 12 kb

Additional file 2: of Molecular assessment of bacterial vaginosis by Lactobacillus abundance and species diversity

The 16S rRNA amplicon sequencing data (DNA sequence, and number of reads) and the family, genus and species identification by the match with the RDP (ribosomal database project) for each 16S rRNA amplicon (V5-V7 region). (XLS 2681 kb