Central lymphocyte counts at time of necropsy.

<p><b>a</b>) Representative immunohistochemistry pictures (20× magnification) of anti-CD3 staining of normal, control, and FTY720 mesenteric lymph node and spleen tissue. <b>b</b>) Quantification of anti-CD3 reactivity by ACIS. * indicates p<0.05 compared to normal, unmanipulated tissue; † indicates p<0.05 compared to control. Data is depicted as mean ± SEM.</p

Peripheral neutrophil counts during hemorrhage and reperfusion periods.

<p>Dashed line indicates end of hemorrhage and beginning of reperfusion period. Data is depicted as mean ± SEM.</p

Hemodynamic profiles during hemorrhage period for FTY720 and vehicle control (in this and subsequent figures control is used for vehicle control) groups.

<p><b>a</b>) Heart rate. <b>b</b>) Mean arterial pressure. <b>c</b>) Cardiac output. There were no statistical differences between the control and experimental hemodynamic profiles.</p

Peripheral leukocyte counts during hemorrhage and reperfusion periods.

<p>Dashed line indicates end of hemorrhage and beginning of reperfusion period. Leukocytes were significantly decreased during the reperfusion period in the FTY720 group (p = 0.03). Data is depicted as mean ± SEM.</p

RT-PCR targets with primer and probe sequences.

<p>RT-PCR targets with primer and probe sequences.</p

Experimental design.

<p>Liver injury was initiated at t = 0. Uncontrolled hemorrhage occurred until 1 hour, at which time the abdomen was packed and temporarily closed (pre-hospital phase). The animal was observed until 2 hours when hospital care was initiated. The liver was repaired and the abdomen definitively closed. The animal was then observed for a total of 72 hours. Blood transfusion was administered as indicated. Necropsy was performed when the animal expired or at 72 hours following euthanasia. The uncontrolled hemorrhage and pre-hospital phases were considered the hemorrhage period, while the hospital care phase was considered the reperfusion period. In the experimental group, FTY720 (0.3 mg/kg diluted in 250 mL of NS)) was administered 15 minutes following liver injury. Vehicle controls were treated with 250 mL of NS.</p

Inflammatory gene transcript expression in liver tissue of FTY720 relative to the control group.

<p>Mean value of relative fold expression is depicted on a logarithmic scale. * indicates p<0.05 compared to control group.</p