Bacterial replication in lungs and spleen of C57BL/6 mice vaccinated with MGM1991, HMA or BCG and challenged with 10⁴ CFU of virulent luminescent <i>M. tuberculosis</i>.

<p>Mean number of bacteria in lungs and spleen of mice vaccinated with 5x10⁴ MGM1991, HMA or BCG or unvaccinated mice (naïve), challenged 12 weeks later with 10⁴ CFU of virulent <i>M.tb</i> by the intratracheal route and sacrificed 4 and 12 weeks after challenge. Results are reported as mean log₁₀ relative light units (RLU) ± SD of 5 mice tested individually; * p < 0.05; ** p < 0.01 (Mann-Whitney test) .</p

Mycobacteria specific intracellular IFN-γ production in CD4⁺ and CD8⁺ T spleen cells of mice vaccinated with MGM1991, HMA or BCG vaccine at 4 weeks after immunization.

<div><p><u>Upper </u><u>panel </u>(A): ICS staining of CD4⁺ spleen cells from C57BL/6 mice vaccinated 4 weeks before with 5x10⁴ CFU of MGM1991, HMA or <i>M. bovis</i> BCG by the subcutaneous route or from unvaccinated (naïve) mice. Mean % of positive cells in each quadrant (of 3 mice tested individually in each group).</p> <p><u>Lower 2 </u><u>panels </u>(B): ICS staining of CD8⁺ spleen cells from C57BL/6 mice vaccinated 4 weeks before with 5x10⁴ CFU of MGM1991, HMA or <i>M. bovis</i> BCG by the subcutaneous route or from unvaccinated (naïve) mice. Results show results obtained in 2 individual mice.</p> <p>^*<i>p</i><0,05 MGM1991 or HMA vs BCG; ^Ŧ<i>P</i><0,05 MGM1991 vs HMA; (Mann-Whitney test).</p></div

Bacterial replication in lungs and spleen of BALB/c mice vaccinated with MGM1991, or BCG and challenged with 10⁵ CFU of virulent luminescent <i>M. tuberculosis</i>.

<p>Mean number of bacteria in lungs and spleen of C57BL/6 mice vaccinated with 5x10⁵CFU of MGM1991 mutant or BCG or unvaccinated mice (naïve), challenged 12 weeks later with 10⁵CFU of virulent <i>M.tb</i> by the intratracheal route and sacrificed 6 or 12 weeks after challenge. Results are reported as mean log₁₀ relative light units (RLU) ± SD of 5 mice tested individually; * <i>P</i> < 0.05; ** <i>P</i> < 0.01 (Mann-Whitney test).</p

Cytokine production of draining lymph node cells of mice vaccinated with MGM1991, HMA or BCG vaccine.

<p>IL-2, IFN-γ TNF-α and IL-17A levels in culture supernatant of pooled inguinal lymph node cells of C57BL/6 mice vaccinated 4 weeks before with 5x10⁴ CFU of MGM1991, HMA or <i>M. bovis</i> BCG by the subcutaneous route or from unvaccinated (naïve) mice and stimulated in vitro with recombinant Ag85A (5 µg/ml), with I-A^b restricted immunodominant peptides spanning aa 241-260 of Ag85A or aa 1-20 of ESAT-6, with purified protein derivative PPD or with culture filtrate from <i>M.tb</i> culture filtrate CF (10 µg/ml). Cytokine levels are expressed in pg/ml (pooled cells from 5 animals/group).</p

IFN-γ and IL-17A ELISPOT cells in spleen and draining lymph nodes of mice vaccinated with MGM1991 or BCG.

<p>IFN-γ and IL-17A producing cells in spleen and inguinal lymph nodes of C57BL/6 mice vaccinated 4 weeks before with 5x10⁴ CFU of MGM1991 or <i>M. bovis</i> BCG by the subcutaneous route or from unvaccinated (naïve) mice and stimulated in vitro with recombinant Ag85A (5 µg/ml), with I-A^b restricted immunodominant peptide spanning aa 1-20 of ESAT-6 or with purified protein derivative PPD from <i>M.tb</i> (10 µg/ml). Cytokine levels are expressed as mean number of spot forming cells/ 10⁶ spleen cells (5 mice tested individually) or as mean number of spot forming cells/ 5x10⁵ lymph node cells (pooled cells from 5 animals/group). * <i>p</i> < 0.05 (Mann-Whitney test).</p

Spleen cell cytokine production in mice vaccinated with MGM1991, HMA or BCG vaccine.

<div><p>IL-2, IFN-γ, TNF-α and IL-17A levels in spleen cell culture supernatant of C57BL/6 mice vaccinated 4 weeks before with 5x10⁴ CFU of MGM1991, HMA or <i>M. bovis</i> BCG by the subcutaneous route or from unvaccinated (naïve) mice and stimulated in vitro with recombinant Ag85A (5 µg/ml) or with I-A^b restricted immunodominant peptides spanning aa 241-260 of Ag85A or aa 1-20 of ESAT-6 (10 µg/ml). Cytokine levels are expressed in pg/ml (mean ± SD of 5 mice tested individually).</p> <p>* <i>p</i> < 0.05; *** <i>p</i> < 0.001; (Mann-Whitney test).</p></div