BICEPS: An improved characterization model for low- and intermediate-mass exoplanets

Context. The number of exoplanets with precise mass and radius measurements is constantly increasing thanks to novel ground- and space-based facilities such as HARPS, ESPRESSO, CHEOPS, and TESS. The accuracy and robustness of the planetary characterization largely depends on the quality of the data, but also requires a planetary structure model, capable of accurately modeling the interior and atmospheres of exoplanets over a large range of boundary conditions. Aims. Our goal is to provide an improved characterization model for planets with masses between 0.5 and 30 Earth masses, equilibrium temperatures below <2000 K, and a wide range of planetary compositions and physical phases. Methods. In this work, we present the Bayesian Interior Characterization of ExoPlanetS (BICEPS) model, which combines an adaptive Markov chain Monte Carlo sampling method with a state-of-the-art planetary structure model. BICEPS incorporates many recently developed equations of state suited for large ranges of pressures and temperatures, a description for solid and molten planetary cores and mantles, a gaseous envelope composed of hydrogen, helium, and water (with compositional gradients), and a non-gray atmospheric model. Results. We find that the usage of updated equations of state has a significant impact on the interior structure prediction. The impact varies, depending on the planetary composition. For dense rocky planets, BICEPS predicts radii a few percent different to prior internal structure models. For volatile rich planets, we find differences of 10% or even larger. When applying BICEPS to a particular exoplanet, TOI-130 b, we inferred a 25% larger water mass fraction and a 15% smaller core than previous models. Conclusions. The presented exoplanet characterization model is a robust method applicable over a large range of planetary masses, compositions, and thermal boundary conditions. We show the importance of implementing state-of-the-art equations of state for the encountered thermodynamic conditions of exoplanets. Hence, using BICEPS improves the predictive strength of the characterization process compared to previous methods.ISSN:0004-6361ISSN:1432-074

Mitotic chromosome length scales in response to both cell and nuclear size

Multicellular development requires that cells reduce in size as a result of consecutive cell divisions without increase in embryo volume. To maintain cellular integrity, organelle size adapts to cell size throughout development. During mitosis, the longest chromosome arm must be shorter than half of the mitotic spindle for proper chromosome segregation. Using high-resolution time-lapse microscopy of living Caenorhabditis elegans embryos, we have quantified the relation between cell size and chromosome length. In control embryos, chromosome length scaled to cell size. Artificial reduction of cell size resulted in a shortening of chromosome length, following a trend predicted by measurements from control embryos. Disturbing the RAN (Ras-related nuclear protein)-GTP gradient decoupled nuclear size from cell size and resulted in chromosome scaling to nuclear size rather than cell size; smaller nuclei contained shorter chromosomes independent of cell size. In sum, quantitative analysis relating cell, nuclear, and chromosome size predicts two levels of chromosome length regulation: one through cell size and a second in response to nuclear size

A theoretical model of cytokinesis implicates feedback between membrane curvature and cytoskeletal organization in asymmetric cytokinetic furrowing

During cytokinesis, the cell undergoes a dramatic shape change as it divides into two daughter cells. Cell shape changes in cytokinesis are driven by a cortical ring rich in actin filaments and nonmuscle myosin II. The ring closes via actomyosin contraction coupled with actin depolymerization. Of interest, ring closure and hence the furrow ingression are nonconcentric (asymmetric) within the division plane across Metazoa. This nonconcentricity can occur and persist even without preexisting asymmetric cues, such as spindle placement or cellular adhesions. Cell-autonomous asymmetry is not explained by current models. We combined quantitative high-resolution live-cell microscopy with theoretical modeling to explore the mechanistic basis for asymmetric cytokinesis in the Caenorhabditis elegans zygote, with the goal of uncovering basic principles of ring closure. Our theoretical model suggests that feedback among membrane curvature, cytoskeletal alignment, and contractility is responsible for asymmetric cytokinetic furrowing. It also accurately predicts experimental perturbations of conserved ring proteins. The model further suggests that curvature-mediated filament alignment speeds up furrow closure while promoting energy efficiency. Collectively our work underscores the importance of membrane–cytoskeletal anchoring and suggests conserved molecular mechanisms for this activity

Kinetochore alignment within the metaphase plate is regulated by centromere stiffness and microtubule depolymerases

During mitosis in most eukaryotic cells, chromosomes align and form a metaphase plate halfway between the spindle poles, about which they exhibit oscillatory movement. These movements are accompanied by changes in the distance between sister kinetochores, commonly referred to as breathing. We developed a live cell imaging assay combined with computational image analysis to quantify the properties and dynamics of sister kinetochores in three dimensions. We show that baseline oscillation and breathing speeds in late prometaphase and metaphase are set by microtubule depolymerases, whereas oscillation and breathing periods depend on the stiffness of the mechanical linkage between sisters. Metaphase plates become thinner as cells progress toward anaphase as a result of reduced oscillation speed at a relatively constant oscillation period. The progressive slowdown of oscillation speed and its coupling to plate thickness depend nonlinearly on the stiffness of the mechanical linkage between sisters. We propose that metaphase plate formation and thinning require tight control of the state of the mechanical linkage between sisters mediated by centromeric chromatin and cohesion

Bosonic String Quantization in Static Gauge

The bosonic string in D dimensional Minkowski space-time is quantized in static gauge. It is shown that the system can be described by D-1 massless free fields constrained on the surface L_m = 0, for m \neq 0, where L_m are the generators of conformal transformations. The free fields are quantized and the physical states are selected by the conditions L_m|phys>=0, for m>0. The Poincar\'e group generators on the physical Hilbert space are constructed and the critical dimension D=26 is recovered from the commutation relations of the boost operators. The equivalence with the covariant quantization is established. A possible generalization to the AdS string dynamics is discussed.Comment: 10 page

S. cerevisiae Chromosomes Biorient via Gradual Resolution of Syntely between S Phase and Anaphase

SummaryFollowing DNA replication, eukaryotic cells must biorient all sister chromatids prior to cohesion cleavage at anaphase. In animal cells, sister chromatids gradually biorient during prometaphase, but current models of mitosis in S. cerevisiae assume that biorientation is established shortly after S phase. This assumption is based on the observation of a bilobed distribution of yeast kinetochores early in mitosis and suggests fundamental differences between yeast mitosis and mitosis in animal cells. By applying super-resolution imaging methods, we show that yeast and animal cells share the key property of gradual and stochastic chromosome biorientation. The characteristic bilobed distribution of yeast kinetochores, hitherto considered synonymous for biorientation, arises from kinetochores in mixed attachment states to microtubules, the length of which discriminates bioriented from syntelic attachments. Our results offer a revised view of mitotic progression in S. cerevisiae that augments the relevance of mechanistic information obtained in this powerful genetic system for mammalian mitosis

Investigating the Regulation of Stem and Progenitor Cell Mitotic Progression by In Situ Imaging

Genome stability relies upon efficacious chromosome congression and regulation by the spindle assembly checkpoint (SAC). The study of these fundamental mitotic processes in adult stem and progenitor cells has been limited by the technical challenge of imaging mitosis in these cells in situ. Notably, how broader physiological changes, such as dietary intake or age, affect mitotic progression in stem and/or progenitor cells is largely unknown. Using in situ imaging of C. elegans adult germlines, we describe the mitotic parameters of an adult stem and progenitor cell population in an intact animal. We find that SAC regulation in germline stem and progenitor cells is distinct from that found in early embryonic divisions and is more similar to that of classical tissue culture models. We further show that changes in organismal physiology affect mitotic progression in germline stem and progenitor cells. Reducing dietary intake produces a checkpoint-dependent delay in anaphase onset, and inducing dietary restriction when the checkpoint is impaired increases the incidence of segregation errors in mitotic and meiotic cells. Similarly, developmental aging of the germline stem and progenitor cell population correlates with a decline in the rate of several mitotic processes. These results provide the first in vivo validation of models for SAC regulation developed in tissue culture systems and demonstrate that several fundamental features of mitotic progression in adult stem and progenitor cells are highly sensitive to organismal physiological changes

ERIS: revitalising an adaptive optics instrument for the VLT

ERIS is an instrument that will both extend and enhance the fundamental diffraction limited imaging and spectroscopy capability for the VLT. It will replace two instruments that are now being maintained beyond their operational lifetimes, combine their functionality on a single focus, provide a new wavefront sensing module that makes use of the facility Adaptive Optics System, and considerably improve their performance. The instrument will be competitive with respect to JWST in several regimes, and has outstanding potential for studies of the Galactic Center, exoplanets, and high redshift galaxies. ERIS had its final design review in 2017, and is expected to be on sky in 2020. This contribution describes the instrument concept, outlines its expected performance, and highlights where it will most excel.Comment: 12 pages, Proc SPIE 10702 "Ground-Based and Airborne Instrumentation for Astronomy VII