Reducing running costs by lowering the probe concentration in a LED Roche LightCycler® 480.

<p>Reducing running costs by lowering the probe concentration in a LED Roche LightCycler® 480.</p

Primers and probes used.

<p>Primers and probes used.</p

Relation of the Cq value and the level of fluorescence in the three different types of Roche LightCycler® 480.

<p>The mean and standard deviation of ten analyses per target is showed by the Cq value and fluorescence in units.</p

Fluorescence intensity in units of gastro-intestinal multiplex rtPCRs (A, B, C, D), measured by LightCycler® 480 instruments type I, II and III.

<p>The mean of ten analyses per target is showed by fluorescence in units. The X-axis represents the types of the PCR instrument and the Y-axis represents the fluorescence intensity in units. MFP: molecular fecal panel. <i>Synechococcus</i> is used as internal control (IC) of the targets and was detected with a probe labelled with a Cy5 dye included in the multiplex rtPCRs MFP1, MFP2, MFP3 and MFP4.</p

Pathogen targets of the gastro-enteritis multiplex rtPCRs with accompanying dyes and probe concentration used in the final rtPCR reaction.

<p>Pathogen targets of the gastro-enteritis multiplex rtPCRs with accompanying dyes and probe concentration used in the final rtPCR reaction.</p

Detection dyes with different excitation and emission spectra are shown for the LightCycler® 480 type I, II and III.

<p>Detection dyes with different excitation and emission spectra are shown for the LightCycler® 480 type I, II and III.</p

Correlation of the probe concentration and the intensity of fluorescence in two different types of Roche LightCycler® 480.

<p>The mean of five analyses per target is showed by fluorescence in units.</p