Multicolor FISH using tándem probes to detect Chromosome alterations in humans cells and populations exposed to genotoxic agents

12 páginas, 2 figuras y 2 tablas estadísticasFluorescence in situ hybridization (FISH) with chromosome- or region-specific DNA probes is being increasingly used in cytogenetic studies to detect aneuploidy in interphase human cells. This technique utilizes chemically modified DNA sequences (probes) which hybridize to distinct regions, often blocks of repetitive DNA, located on specific chromosomes. Hybridization with these probes in situ results in the staining of a compact chromosomal región which can be easily detected on metaphase chromosomes or within interphase nuclei. The number of chromosomes within a given cell is then determined by counting the number of hybridized regions. Where conventional cytogenetics is limited to actively proliferating cells or those which could be stimulated to divide in vitro such as peripheral blood lymphocytes, FISH studies with centromeric probes can be conducted on interphase cells, significantly increasing the types of cells and tissues available for analysis.Peer reviewe