Плоскоклеточный рак у пациентки с эрозивно-язвенной формой красного плоского лишая слизистой оболочки полости рта

Lichen planus refers to dermatoses with frequent, often isolated, lesions of the oral mucosa. In the presence of rashes only on the oral mucosa, the diagnosis is difficult. At the same time, erosive-ulcerative forms of the disease present the greatest differential diagnostic difficulties. Cases that do not heal for a long time and are not amenable to treatment should be especially alert to the doctor due to the risk of malignancy. The article describes a rare clinical case of squamous cell carcinoma of the oral mucosa in a 52-year-old patient with an erosive-ulcerative form of lichen planus of the oral cavity, characterized by a chronic long-term course, torpidity to therapy and simultaneous damage to the genital mucosa. The presented case emphasizes the importance of a thorough clinical examination, with a histological examination of the biopsy in order to monitor changes in the lesions.Красный плоский лишай относится к дерматозам с частым, нередко изолированным поражением слизистой оболочки полости рта. При наличии высыпаний только на слизистой оболочке полости рта диагностика отличается сложностью. При этом наибольшие дифференциально-диагностические трудности представляют эрозивно-язвенные формы заболевания. Особенно должны настораживать врача длительно не заживающие и не поддающиеся лечению случаи в связи с опасностью озлокачествления. В статье описан редкий клинический случай плоскоклеточного рака слизистой оболочки полости рта у пациентки 52 лет с эрозивно-язвенной формой красного плоского лишая полости рта, отличающейся хроническим длительным течением, торпидностью к терапии и одновременным поражением слизистой оболочки гениталий. Представленный случай подчеркивает важность тщательного клинического осмотра с проведением гистологического исследования биоптата с целью контроля за изменениями очагов поражения

Estudio de la etiología, patogenia y diagnóstico de las dermatomicosis del cuero cabelludo, uñas, pies, manos, piel lisa y dermatofitosis inguinal

Introduction: The increased incidence and preponderance of dermatomycosis cause the issue of determining antimycotic agents of systemic and external action to be particularly pertinent, considering the etiology, clinical form, and majority of mycoses, the sensitivity of pathogens to them, and the existence of concurrent conditions. The study aims to analyze the etiology, pathogenesis, and diagnosis of dermatomycosis of the scalp, nails, feet, hands, smooth skin, and inguinal dermatophytosis. Patients and methods: To meet the aim of the study, it is attempted to generate an algorithm for pharmaceutical counseling of patients suffering from various types of dermatomycosis. Over the span of five months, a survey was carried out of 25 visitors to the pharmacy with a diagnosis of dermatomycosis. Results: Based on the results, it was revealed that most of the visitors suffer from dermatomycosis of the feet - 10 people (40%), the second in terms of incidence is onychomycosis - 5 people (19%), the third in terms of incidence is dermatomycosis of the groin area - 4 people (15%), the fourth in the incidence is dermatomycosis of the scalp - 4 people (14%). Conclusion: To treat different types of dermatomycosis, various antifungal drugs in different dosage forms can be used. For each type of disease, a specific course of treatment is selected. The duration of the course of treatment depends on the skin lesion area, the duration of the disease, and the presence of complications.Introducción: El aumento de la incidencia y preponderancia de las dermatomicosis hace que el tema de la determinación de agentes antimicóticos de acción sistémica y externa sea particularmente pertinente, considerando la etiología, forma clínica y mayoría de las micosis, la sensibilidad de los patógenos a las mismas y la existencia de condiciones. El estudio tiene como objetivo analizar la etiología, patogenia y diagnóstico de las dermatomicosis del cuero cabelludo, uñas, pies, manos, piel lisa y dermatofitosis inguinal. Pacientes y métodos: Para cumplir con el objetivo del estudio, se intenta generar un algoritmo para el asesoramiento farmacéutico de pacientes que padecen diversos tipos de dermatomicosis. Durante cinco meses se realizó una encuesta a 25 visitantes de la farmacia con diagnóstico de dermatomicosis. Resultados: Con base en los resultados, se reveló que la mayoría de los visitantes padecen dermatomicosis de los pies - 10 personas (40%), el segundo en cuanto a incidencia es la onicomicosis - 5 personas (19%), el tercero en cuanto a la incidencia es dermatomicosis del área de la ingle - 4 personas (15%), el cuarto en la incidencia es dermatomicosis del cuero cabelludo - 4 personas (14%). Conclusión: Para tratar diferentes tipos de dermatomicosis, se pueden utilizar diversos fármacos antifúngicos en diferentes formas de dosificación. Para cada tipo de enfermedad, se selecciona un curso de tratamiento específico. La duración del curso del tratamiento depende del área de la lesión cutánea, la duración de la enfermedad y la presencia de complicaciones

Insights into the structure and function of Est3 from the Hansenula polymorpha telomerase

© 2020, The Author(s). Telomerase is a ribonucleoprotein enzyme, which maintains genome integrity in eukaryotes and ensures continuous cellular proliferation. Telomerase holoenzyme from the thermotolerant yeast Hansenula polymorpha, in addition to the catalytic subunit (TERT) and telomerase RNA (TER), contains accessory proteins Est1 and Est3, which are essential for in vivo telomerase function. Here we report the high-resolution structure of Est3 from Hansenula polymorpha (HpEst3) in solution, as well as the characterization of its functional relationships with other components of telomerase. The overall structure of HpEst3 is similar to that of Est3 from Saccharomyces cerevisiae and human TPP1. We have shown that telomerase activity in H. polymorpha relies on both Est3 and Est1 proteins in a functionally symmetrical manner. The absence of either Est3 or Est1 prevents formation of a stable ribonucleoprotein complex, weakens binding of a second protein to TER, and decreases the amount of cellular TERT, presumably due to the destabilization of telomerase RNP. NMR probing has shown no direct in vitro interactions of free Est3 either with the N-terminal domain of TERT or with DNA or RNA fragments mimicking the probable telomerase environment. Our findings corroborate the idea that telomerase possesses the evolutionarily variable functionality within the conservative structural context