SCA-PVNet: Self-and-Cross Attention Based Aggregation of Point Cloud and Multi-View for 3D Object Retrieval

To address 3D object retrieval, substantial efforts have been made to generate highly discriminative descriptors of 3D objects represented by a single modality, e.g., voxels, point clouds or multi-view images. It is promising to leverage the complementary information from multi-modality representations of 3D objects to further improve retrieval performance. However, multi-modality 3D object retrieval is rarely developed and analyzed on large-scale datasets. In this paper, we propose self-and-cross attention based aggregation of point cloud and multi-view images (SCA-PVNet) for 3D object retrieval. With deep features extracted from point clouds and multi-view images, we design two types of feature aggregation modules, namely the In-Modality Aggregation Module (IMAM) and the Cross-Modality Aggregation Module (CMAM), for effective feature fusion. IMAM leverages a self-attention mechanism to aggregate multi-view features while CMAM exploits a cross-attention mechanism to interact point cloud features with multi-view features. The final descriptor of a 3D object for object retrieval can be obtained via concatenating the aggregated features from both modules. Extensive experiments and analysis are conducted on three datasets, ranging from small to large scale, to show the superiority of the proposed SCA-PVNet over the state-of-the-art methods

Listen to genes : dealing with microarray data in the frequency domain

Background: We present a novel and systematic approach to analyze temporal microarray data. The approach includes normalization, clustering and network analysis of genes. Methodology: Genes are normalized using an error model based uniform normalization method aimed at identifying and estimating the sources of variations. The model minimizes the correlation among error terms across replicates. The normalized gene expressions are then clustered in terms of their power spectrum density. The method of complex Granger causality is introduced to reveal interactions between sets of genes. Complex Granger causality along with partial Granger causality is applied in both time and frequency domains to selected as well as all the genes to reveal the interesting networks of interactions. The approach is successfully applied to Arabidopsis leaf microarray data generated from 31,000 genes observed over 22 time points over 22 days. Three circuits: a circadian gene circuit, an ethylene circuit and a new global circuit showing a hierarchical structure to determine the initiators of leaf senescence are analyzed in detail. Conclusions: We use a totally data-driven approach to form biological hypothesis. Clustering using the power-spectrum analysis helps us identify genes of potential interest. Their dynamics can be captured accurately in the time and frequency domain using the methods of complex and partial Granger causality. With the rise in availability of temporal microarray data, such methods can be useful tools in uncovering the hidden biological interactions. We show our method in a step by step manner with help of toy models as well as a real biological dataset. We also analyse three distinct gene circuits of potential interest to Arabidopsis researchers

Intracoronary artery retrograde thrombolysis combined with percutaneous coronary interventions for ST-segment elevation myocardial infarction complicated with diabetes mellitus: A case report and literature review

BackgroundThe management of a large thrombus burden in patients with acute myocardial infarction and diabetes is still a worldwide problem.Case presentationA 74-year-old Chinese woman presented with ST-segment elevation myocardial infarction (STEMI) complicated with diabetes mellitus and hypertension. Angiography revealed massive thrombus formation in the mid-segment of the right coronary artery leading to vascular occlusion. The sheared balloon was placed far from the occlusion segment and urokinase (100,000 u) was administered for intracoronary artery retrograde thrombolysis, and thrombolysis in myocardial infarction (TIMI) grade 3 blood flow was restored within 7 min. At last, one stent was accurately implanted into the culprit’s vessel. No-reflow, coronary slow flow, and reperfusion arrhythmia were not observed during this process.ConclusionIntracoronary artery retrograde thrombolysis (ICART) can be effectively and safely used in patients with STEMI along with diabetes mellitus and hypertension, even if the myocardial infarction exceeds 12 h (REST or named ICART ClinicalTrials.gov number, ChiCTR1900023849)

31st Annual Meeting and Associated Programs of the Society for Immunotherapy of Cancer (SITC 2016) : part two

Background The immunological escape of tumors represents one of the main ob- stacles to the treatment of malignancies. The blockade of PD-1 or CTLA-4 receptors represented a milestone in the history of immunotherapy. However, immune checkpoint inhibitors seem to be effective in specific cohorts of patients. It has been proposed that their efficacy relies on the presence of an immunological response. Thus, we hypothesized that disruption of the PD-L1/PD-1 axis would synergize with our oncolytic vaccine platform PeptiCRAd. Methods We used murine B16OVA in vivo tumor models and flow cytometry analysis to investigate the immunological background. Results First, we found that high-burden B16OVA tumors were refractory to combination immunotherapy. However, with a more aggressive schedule, tumors with a lower burden were more susceptible to the combination of PeptiCRAd and PD-L1 blockade. The therapy signifi- cantly increased the median survival of mice (Fig. 7). Interestingly, the reduced growth of contralaterally injected B16F10 cells sug- gested the presence of a long lasting immunological memory also against non-targeted antigens. Concerning the functional state of tumor infiltrating lymphocytes (TILs), we found that all the immune therapies would enhance the percentage of activated (PD-1pos TIM- 3neg) T lymphocytes and reduce the amount of exhausted (PD-1pos TIM-3pos) cells compared to placebo. As expected, we found that PeptiCRAd monotherapy could increase the number of antigen spe- cific CD8+ T cells compared to other treatments. However, only the combination with PD-L1 blockade could significantly increase the ra- tio between activated and exhausted pentamer positive cells (p= 0.0058), suggesting that by disrupting the PD-1/PD-L1 axis we could decrease the amount of dysfunctional antigen specific T cells. We ob- served that the anatomical location deeply influenced the state of CD4+ and CD8+ T lymphocytes. In fact, TIM-3 expression was in- creased by 2 fold on TILs compared to splenic and lymphoid T cells. In the CD8+ compartment, the expression of PD-1 on the surface seemed to be restricted to the tumor micro-environment, while CD4 + T cells had a high expression of PD-1 also in lymphoid organs. Interestingly, we found that the levels of PD-1 were significantly higher on CD8+ T cells than on CD4+ T cells into the tumor micro- environment (p < 0.0001). Conclusions In conclusion, we demonstrated that the efficacy of immune check- point inhibitors might be strongly enhanced by their combination with cancer vaccines. PeptiCRAd was able to increase the number of antigen-specific T cells and PD-L1 blockade prevented their exhaus- tion, resulting in long-lasting immunological memory and increased median survival

Risk prediction of bronchopulmonary dysplasia in preterm infants by the nomogram model

Backgrounds and AimsBronchopulmonary dysplasia (BPD) has serious immediate and long-term sequelae as well as morbidity and mortality. The objective of this study is to develop a predictive model of BPD for premature infants using clinical maternal and neonatal parameters.MethodsThis single-center retrospective study enrolled 237 cases of premature infants with gestational age less than 32 weeks. The research collected demographic, clinical and laboratory parameters. Univariate logistic regression analysis was carried out to screen the potential risk factors of BPD. Multivariate and LASSO logistic regression analysis was performed to further select variables for the establishment of nomogram models. The discrimination of the model was assessed by C-index. The Hosmer-Lemeshow test was used to assess the calibration of the model.ResultsMultivariate analysis identified maternal age, delivery option, neonatal weight and age, invasive ventilation, and hemoglobin as risk predictors. LASSO analysis selected delivery option, neonatal weight and age, invasive ventilation, hemoglobin and albumin as the risk predictors. Both multivariate (AUC = 0.9051; HL P = 0.6920; C-index = 0.910) and LASSO (AUC = 0.8935; HL P = 0.7796; C-index = 0.899) - based nomograms exhibited ideal discrimination and calibration as confirmed by validation dataset.ConclusionsThe probability of BPD in a premature infant could be effectively predicted by the nomogram model based on the clinical maternal and neonatal parameters. However, the model required external validation using larger samples from multiple medical centers

EFFECT OF DIFFERENT MAINTENANCE DOSES OF CAFFEINE CITRATE ON EARLY-STAGE PULMONARY FUNCTION AND COMPLICATIONS IN PRETERM INFANTS

Objective To investigate the effect of different maintenance doses of caffeine citrate treatment on early-stage pulmonary function and complications in preterm infants. Methods A total of 78 preterm infants with a gestational age of 28 weeks to 33+6 weeks who were admitted to our hospital from April 2019 to January 2022 and did not need invasive mechanical ventilation after birth were enrolled as subjects, and they were divided into high-maintenance-dose caffeine citrate group with 40 infants and low-maintenance-dose caffeine citrate group with 38 infants using a random number table. Both groups were given peripheral intravenous pumping of caffeine citrate at a loading dose of 20 mg/(kg·d) at 2 h after birth, and then the high and low maintenance dose groups were given caffeine citrate at a maintenance dose of 10 mg/(kg·d) and 5 mg/(kg·d), respectively, after 24 h. The two groups were compared in terms of pulmonary function, complications (necrotizing enterocolitis, retinopathy of prematurity, and intraventricular hemorrhage), and adverse reactions (feeding intolerance, hyperglycemia, and tachycardia) on days 7 and 14 after birth and at the corrected gestational age of 40 weeks. Results Compared with the low maintenance dose group, the high maintenance dose group had significantly higher tidal volume, ratio of time to peak tidal expiratory flow to total expiratory time, and ratio of volume to peak tidal expiratory flow to total expiratory volume on days 7 and 14 after birth and at the corrected gestational age of 40 weeks, as well as a significantly higher respiratory rate on days 7 and 14 after birth (t=2.107-4.128,P&lt;0.05). There were no significant differences in the incidence rates of complications and adverse reactions between the high and low maintenance dose groups (P&gt;0.05). Conclusion A high maintenance dose of caffeine citrate can significantly improve early-stage pulmonary function in preterm infants without increasing related complications and adverse reactions

Optimization of Steam Distillation Process for Volatile Oils from <i>Forsythia suspensa</i> and <i>Lonicera japonica</i> according to the Concept of Quality by Design

In this study, the process of steam distillation to collect volatile oils from Forsythia suspensa (F. suspensa) and Lonicera japonica (L. japonica) was optimized according to the concept of quality by design. First, the liquid/material ratio, distillation time, and collection temperature were identified as critical process parameters by a review of the literature and single-factor experiments. Then, a Box–Behnken design was used to study the quantitative relationship between the three process parameters, two raw material properties, and the yield of volatile oil. A mathematical model was established with an R2 value exceeding 0.90. Furthermore, the design space of the volatile oil yield was calculated by a probability-based method. The results of a verification experiment showed that the model was accurate and the design space was reliable. A total of 16 chemical constituents were identified in the volatile oil from mixtures of F. suspensa and L. japonica. The content of β-pinene was the highest (54.75%), and the composition was similar to that of the volatile oil of F. suspensa. The results showed that when F. suspensa and L. japonica were distilled together, the main contribution to the volatile oil was from F. suspensa. The volatile oil yield from the combination of F. suspensa and L. japonica was not higher than that from L. japonica