Expression of <i>RBBP6</i> in colon tumorous tissues and adjacent normal mucosa.

<p><b>A.</b> Relative expression of <i>RBBP6</i> gene in a series of 40 matched colon cancerous tissue specimens compared with that in normal mucosa specimens. A logarithmic scale of 2− ^ΔΔCT was used to represent the fold change in quantitative real-time PCR detection. <b>B.</b> Western blotting analysis of RBBP6 protein expression in representative 4 paired colon tumor tissues. GAPDH was used as the loading control.</p

Immunohistochemical staining for RBBP6 expression in normal and colon cancer tissues.

<p>(A) Negative RBBP6 expression in normal colonic epithelium and (B) well-differentiated tumor. (C) Weak RBBP6 staining in a well-differentiated colon tumor. (D) Diffused, intense RBBP6 staining in moderately- and (E) poorly differentiated colon tumors. (F) Strong RBBP6 staining in a colon cancer lymph node metastasis sample. Original magnification ×200 (×400 for inset images).</p

Kaplan-Meier analyses with a log rank test of survival.

<p><b>A.</b> Disease-free survival (DFS) and overall survival (OS) of patients in relation with RBBP6 expression levels that were determined by immunohistochemical staining. <b>B.</b> DFS was significantly better in patients with TP53-negative tumors than in those with TP53-positive tumors (<i>P = </i>0.036). <b>C.</b> DFS and OS were significantly lower in patients with RBBP6− and TP53-positive tumors than in those with RBBP6− and TP53-negative tumors (<i>P</i><0.001 for both).</p

RBBP6 and TP53 immunohistochemical staining for protein expression in normal colonic mucosa, cancerous tissue, and lymph node metastasis.

*<p><i>P</i>-value is based on chi-square test; LNM: Lymph node metastasis.</p

Expression of RBBP6 and

<p><b>TP53.</b> Representative photographs of RBBP6 and TP53 expression in normal colon, colon tumor, and nodal metastasis specimens. RBBP6 expression was more frequently detected in specimens that stained positively for mutant TP53. Original magnification ×400 (×50 for inset images).</p

Univariate and multivariate Cox proportional hazard models for overall survival (OS) and disease-free survival (DFS).

<p>HR: Hazard ratio; CI: Confidence interval.</p>*<p><i>P</i><0.05 indicated that 95% CI of HR was not including 1.</p

Association between clinicopathologic features and RBBP6 or TP53 protein expression.

*<p><i>P</i><0.05 indicates a significant association among the variables.</p>**<p>The significant difference of correlation between RBBP6 and TP53 based on Spearman test.</p

Additional file 5: Table S5. of miR-4775 promotes colorectal cancer invasion and metastasis via the Smad7/TGFβ-mediated epithelial to mesenchymal transition

Correlations between miR-4775 expression and E-cadherin, N-cadherin and vimentin staining in tumor tissues from 544 CRC patients. (DOCX 16 kb

Additional file 4: Table S4. of miR-4775 promotes colorectal cancer invasion and metastasis via the Smad7/TGFβ-mediated epithelial to mesenchymal transition

Multivariate Cox proportional hazard analyses of miR-4775 expression, T stage, Lymph node metastasis and distant metastasis in association with DFS and OS in 544 CRC patients. (DOCX 16 kb