Immunohistochemistry of the TH-positive cells in the substantia nigra of the monkeys in two groups.

<p>Scale bar: 100 µm.</p

Detction of the expression of Lmx1α by western blot.

<p>Lane 1, was loaded with the cell lysis infected with Ad-Lmx1α. Lane 2, was loaded with the medium infected with Ad-Lmx1α. Lane 3, was loaded with the cell lysis from control hUC-MSCs. Lane 4, was loaded with the medium from control hUC-MSCs.</p

Expansion of hUC-MSCs cultured <i>in vitro</i>.

<p>(A) Morphology of hUC-MSCs primary cultured for 20 days. Scale bar: 40 µm. (B) Morphology of hUC-MSCs cultured at the 7^th generation. Scale bar: 100 µm.</p

The expression of neural-specific marker containing TH, β-tubulinIII, MAP-2, NSE, Nestin and Lmx1α in hUC-MSCs induced 21 days was analyzed by immunofluorescence.

<p>Scale bar: 100 µm.</p

Detection of NTN secretion in 24 h, 48 h, 72 h and 96 h of hUC-MSCs infected with Ad-NGFpreproNTN and control hUC-MSCs by ELISA (* p<0.05).

<p>Detection of NTN secretion in 24 h, 48 h, 72 h and 96 h of hUC-MSCs infected with Ad-NGFpreproNTN and control hUC-MSCs by ELISA (* p<0.05).</p

The expression of neural-specific marker containing TH, β-tubulinIII, MAP-2, NSE, Nestin and Lmx1α in hUC-MSCs induced 7 days was analyzed by immunofluorescence.

<p>Scale bar: 100 µm.</p

Differentiation of hUC-MSCs into osteoblasts <i>in vitro</i>.

<p>(A, D) Morphology of hUC-MSCs cultured in osteogenic medium for 21 days. (B, E) <i>In vitro</i> differentiation of hUC-MSCs into osteoblasts, were shown by positive alizarin red (B) and ALP staining (E) of calcified extracellular matrix. (C, F) Staining was not observed in control hUC-MSCs cultured in growth medium. Scale bar: 100 µm.</p

Immunohistochemistry of the BrdU-ir cells in the substantia nigra of the monkeys in two groups.

<p>Scale bar: 100 µm.</p

The transcription of NTN and Lmx1α in hUC-MSCs was analyzed by RT-PCR.

<p>Lane 1, the PCR product of Lmx1α in Ad-Lmx1α infected hUC-MSCs. Lane 2, the PCR product of β-actin in Ad-Lmx1α infected hUC-MSCs. Lane 3, the product of NTN in Ad-NGFpreproNTN infected hUC-MSCs. Lane 4, the product of β-actin in Ad-NGFpreproNTN infected hUC-MSCs. Lane 5, the product of Lmx1α in control hUC-MSCs. Lane 6, the product of NTN in control hUC-MSCs. Lane 7, the product of β-actin in control hUC-MSCs, DNA marker DL,2000.</p

The specific mRNA of induced hUC-MSCcs.

<p>Transcription of neural-specific genes like TH, Msx-1, Nestin, MAP-2, Lmx1β, Foxα2, Pitx3, DAT, β-tubulin III, Nurrl, EN1 and NES was analyzed by real-time PCR. All samples were normalized with β-actin (* p<0.05 compared with non-induecd cells; # p<0.05 compared with cells induced for 7 days).</p