Additional file 2: of Association between diet and gallstones of cholesterol and pigment among patients with cholecystectomy: a case-control study in Korea

Scree plot. (DOC 69 kb

Primer sequences for the generation of truncated mutants of the p37 protein.

<p>Primer sequences for the generation of truncated mutants of the p37 protein.</p

CA27 recognizes the residues 216–246 of the p37 protein.

<p>(A) Schematic diagram of recombinant p37 fragments (residues 183–219, 216–246, and 216–240). (B) Individual fusion proteins were expressed in <i>E</i>. <i>coli</i> as fusion proteins with GST tag at the N-terminus and stained with Coomassie Brilliant Blue R250 after SDS-PAGE. (C-D) Western blot analyses of GST-P37 fusion proteins with α-GST (C) and CA27 antibodies (D). Mp37 represents the mycoplasmal p37 protein from the extract of mycoplasma-infected cancer cells. The asterisks indicate partial degradation of GST-p37 fusion proteins.</p

CA27 recognizes the residues 183–246 of the mycoplasmal p37 protein expressed as GST-fusion proteins.

<p>(A) Schematic diagram of recombinant p37 fragments (residues 42–190, 183–246, 241–313, and 315–403). (B) Individual fusion proteins were expressed in <i>E</i>. <i>coli</i> as fusion proteins with GST tag at the N-terminus and stained with Coomassie Brilliant Blue R250 after SDS-PAGE. (C-D) Western blot analyses of GST-P37 fusion proteins with α-GST (C) and CA27 antibodies (D). Mp37 represents the mycoplasmal p37 protein from the extract of mycoplasma-infected cancer cells. The asterisks indicate partial degradation of GST-p37 fusion proteins.</p

CA27 recognizes the residues 226–246 of the p37 protein, and the binding is SDS-resistant but urea-sensitive.

<p>(A) Schematic diagram of recombinant p37 fragments (residues 216–246, 226–246, and 216–240). (B) Individual fusion proteins were expressed in <i>E</i>. <i>coli</i> as fusion proteins with GST tag at the N-terminus and stained with Coomassie Brilliant Blue R250 after SDS-PAGE. (C-D) Western blot analyses of GST-p37 fusion proteins with α-GST (C) and CA27 antibodies (D). Mp37 represents the mycoplasmal p37 protein from the extract of mycoplasma-infected cancer cells. The asterisks indicate partial degradation of GST-p37 fusion proteins. (E-F) Western blot analysis of the purified GST-p37 fusion proteins in the presence or absence of urea with α-GST (E) and CA27 antibodies (F).</p

List of 6 MAbs against the mycoplasma p37 protein.

<p>List of 6 MAbs against the mycoplasma p37 protein.</p

CA63 recognizes the residues 226–246 of the p37 protein.

<p>(A) Schematic diagram of recombinant p37 fragments (residues 216–246, 226–246, and 216–240). (B) Individual fusion proteins were expressed in <i>E</i>. <i>coli</i> as fusion proteins with GST tag at the N-terminus and stained with Coomassie Brilliant Blue R250 after SDS-PAGE. (C-D) Western blot analyses of GST-p37 fusion proteins with α-GST (C) and CA27 antibodies (D). Mp37 represents the mycoplasmal p37 protein from the extract of mycoplasma-infected cancer cells. The asterisks indicate partial degradation of GST-p37 fusion proteins.</p

CA279 recognizes the residues 226–246 of the p37 protein.

<p>(A) Schematic diagram of recombinant p37 fragments (residues 216–246, 226–246, and 216–240). (B) Individual fusion proteins were expressed in <i>E</i>. <i>coli</i> as fusion proteins with GST tag at the N-terminus and stained with Coomassie Brilliant Blue R250 after SDS-PAGE. (C-D) Western blot analyses of GST-p37 fusion proteins with α-GST (C) and CA27 antibodies (D). Mp37 represents the mycoplasmal p37 protein from the extract of mycoplasma-infected cancer cells. The asterisks indicate partial degradation of GST-p37 fusion proteins.</p