Kaplan-Meier overall survival curves of NPC patients with expression of p-Mnk1 and p-eIF4E protein and different clinicopathological characteristics.

<p>Kaplan-Meier analysis to plot the survival curve of all 272 NPC patients with expression of p-Mnk1 and p-eIF4E and different clinicopathological characteristics and statistical significance was assessed using the log-rank test. Fig. 3A. Kaplan-Meier curves showed worse overall survival for p-Mnk1–positive patients compared with p-Mnk1–negative patients (P<0.001, two sided). Fig. 3B. Kaplan-Meier curves showed worse survival for p-eIF4E–positive patients compared with p-eIF4E–negative patients (P = 0.004, two sided). Fig. 3C. NPC patients with clinical stage III and IV were significantly related to poor prognosis compared to those patients with clinical stage I and II (P = 0.003, two sided). Fig. 3D. NPC patients with lymph node metastasis were significantly related to poor prognosis compared to those patients without lymph node metastasis (P<0.001, two sided). Fig. 3E NPC patients with combination chemotherapy and radiotherapy were significantly related to good prognosis compared to patients with chemotherapy and radiotherapy alone (P = 0.006, two sided). Fig. 3F. Histological types of NPC patients were no significantly related to their prognosis (P>0.05, two sided).</p

Association between expression of p-Mnk1 and p-eIF4E protein and clinicopathological features of NPC (n = 272).

<p>Abbreviations: NS, non significant; DNKC: Differentiated non-keratinized carcinoma, UDC: Undifferentiated carcinoma; LN, lymph node; LN, LNM, lymph node metastasis.</p

Expression of p-Mnk1 and p-eIF4E protein in NPC and non-cancerous nasopharyngeal epithelial tissues was detected by immunohistochemistry.

<p>The expression of p-Mnk1 and p-eIF4E protein was detected by immunohistochemistry using specific antibodies as described in the section of materials and methods. Strong positive expression of p-Mnk1 was found in nuclei of NPC (Fig. 1A, 20×, IHC, AEC staining). Low and weak nuclear positive expression of p-Mnk1 was showed in the non-cancerous nasopharyngeal epithelial cells (Fig. 1B, 20×, IHC, AEC staining). Strong positive expression of p-eIF4E in the cytoplasm of NPC (Fig. 1C, 20×, IHC, AEC staining). Weak positive expression of p-eIF4E in the cytoplasm of the non-cancerous nasopharyngeal epithelial cells (Fig. 1D, 20×, IHC, AEC staining).</p

The pairwise association between expression of p-Mnk1 and p-eIF4E protein in the 272 cases of NPC.

<p>The pairwise association between expression of p-Mnk1 and p-eIF4E protein in the 272 cases of NPC.</p

Summary of multivariate analysis of Cox proportional hazards model for overall survival in 272 cases of NPC patients.

<p>Abbreviations: DNKC: Differentiated non-keratinized carcinoma, UDC: Undifferentiated carcinoma; LN, lymph node; LNM, lymph node metastasis. Chemotherapy: CT, Radiotherapy: RT.</p

Additional file 1: Table S1. of Genome-wide analyses of long noncoding RNA expression profiles correlated with radioresistance in nasopharyngeal carcinoma via next-generation deep sequencing

Primer sequences of the lncRNAs and mRNAs. (XLS 26 kb

Kaplan-Meier overall survival curves of NPC patients with expression of p-Akt, p-4EBP1 and p-p70S6K proteins and different clinicopathological characteristics.

<p>Kaplan-Meier analysis to plot the survival curve of 248 cases of NPC with expression of p-Akt, p-4EBP1 and p-p70S6K and clinicopathological characteristics and statistical significance were assessed using the log-rank test. Fig. 2A: Positive expression of p-Akt had no significantly correlation with overall survival rates of NPC patients (<i>P</i>>0.05, two sided). Fig. 2B: Kaplan-Meier curves showed worse overall survival rates for NPC patients with positive expression of p-p70S6K protein compared to patients with p-p70S6K negative staining (<i>P</i> = 0.038, two sided). Fig. 2C: Kaplan-Meier curves showed worse overall survival rates for NPC patients with positive expression of p-4EBP1 protein compared to patients with p-4EBP1 negative staining (<i>P</i> = 0.037, two sided). Fig. 2D: Kaplan-Meier curves showed worse overall survival rates for NPC patients with either of p-Akt, p-p70S6K and p-4EBP1 positive expression compared to patients with common negative staining of three proteins above (<i>P</i> = 0.001, two sided).</p

Association between expression of p-Akt, p-p70S6K and p-4EBP1 and NPC clinical pathological features (N = 248).

<p><b>Abbreviations:</b> DNKC: Differentiated non-keratinized nasopharyngeal carcinoma; UDNC: Undifferentiated non-keratinized nasopharyngeal carcinoma; LN: lymph node; LNM, lymph node metastasis; P: positive; N: negative.</p><p><b>*</b>:P⁺: either of positive expression of p-Akt, p-p70S6K and p-4EBP1; N⁻: common negative staining of three proteins.</p><p>Association between expression of p-Akt, p-p70S6K and p-4EBP1 and NPC clinical pathological features (N = 248).</p

Expression of Flot-2 protein in NPC, atypical hyperplasia epithelial cells and control nasopharyngeal epithelial cells was detected by IHC.

<p>The expression of Flot-2 protein was detected by immunohistochemistry using specific antibody as described in the section of materials and methods. Strong positive expression of Flot-2 protein was found in NPC cell membranes (Fig 1A, 20×, IHC, DAB staining). Weak Flot-2 membrane-staining was showed in the atypical hyperplasia epithelial cells (Fig 1B, 20×, IHC, DAB staining). Negative staining of Flot-2 was showed that in the control nasopharyngeal epithelial cells (Fig 1C, 20×, IHC, DAB staining). Negative control showed no Flot-2 staining in the NPC cells (Fig 1D, 20×, IHC, DAB staining).</p

Summary of multivariate analysis of Cox proportional hazard regression for overall survival in 248 cases of NPC.

<p><b>Abbreviations:</b> LNM, lymph node metastasis; CI, confidence interval.</p><p><b>Note:</b> multivariate analysis of Cox regression,*:p<0.05.</p><p>Summary of multivariate analysis of Cox proportional hazard regression for overall survival in 248 cases of NPC.</p