MOESM1 of Engineering Corynebacterium glutamicum for violacein hyper production

Additional file 1: Table S1. Strains, plasmids and oligonucleotides used in this study. Figure S1. The linear relationship between Absorbance at 570 nm a nd concentration of crude violacein. Figure S2. Batch cultivations of C. glutamicum in LBHIS broth

Macro- and meso-mechanic investigations on the mechanical properties of heterostructured Al matrix composites featuring intragranular reinforcement

Aluminum matrix composites (AMCs) reinforced with intragranular nano-sized Al2O3 were used as model material to investigate the effects of intragranular nano reinforcement on the mechanical properties at macro- and meso- levels. The results revealed that intragranular Al2O3 effectively facilitates the dislocation multiplication, which in turn enables the grain interior to endure high levels of plastic strain and consequently alleviates stress concentration at the grain boundary. Furthermore, intragranular Al2O3 leads to a collective enhancement in the intrinsic mechanical properties across domains of varying sizes, thereby contributing to the coordinated plastic deformation of heterogeneous grains, and enhancing the creep resistance of composite.</p

MOESM1 of Genome editing of Ralstonia eutropha using an electroporation-based CRISPR-Cas9 technique

Additional file 1: Figure S1. rfp editing identified by agarose gel electrophoresis and sequencing. Figure S2. pBBR1-Cas9-rfpF-rfpR clearance. Figure S3. Four genes edited by CRISPR-Cas9. Table S1. Putative restriction endonuclease genes in R. eutropha H16. Table S2. Genes related to putative NHEJ in R. eutropha. Table S3. List of plasmids used in this study. Table S4. List of main primers used in this study

MOESM2 of Genome editing of Ralstonia eutropha using an electroporation-based CRISPR-Cas9 technique

Additional file 2. Profile and sequence of rfp editing plasmid pBBR1-Cas9-rfpF-rfpR

<i>In Situ</i> Carbon-Coated Yolk–Shell V₂O₃ Microspheres for Lithium-Ion Batteries

Metal oxide-based materials with yolk–shell morphology have been intensively investigated as important anodes for Li-ion batteries due to their large ion storage ability, high safety, and excellent cycling stability. In this work, <i>in situ</i> carbon-coated yolk–shell V₂O₃ microspheres were synthesized via a template-free polyol solvothermal method. The growth of yolk–shell microspheres underwent coordination and polymerization, followed by an inside–out Ostwald-ripening process and further calcination in N₂ atmosphere. The thin amorphous carbon layers coating on the microspheres’ surface came from polyol frameworks which could protect V₂O₃ during the charge–discharge process and led to a better stability in Li-ion batteries. The <i>in situ</i> carbon-coated yolk–shell V₂O₃ microspheres showed a capacity of 437.5 mAh·g^–1 after 100 cycles at a current density of 0.1 A·g^–1, which was 92.6% of its initial capability (472.5 mAh·g^–1). They were regarded as excellent electrode materials for lithium-ion batteries and exhibit good electrochemistry performance and stability

MOESM2 of Development of a fast and easy method for Escherichia coli genome editing with CRISPR/Cas9

Additional file 2. Plasmids profiles and sequences

Poly(d‑amino acid) Nanoparticles Target <i>Staphylococcal</i> Growth and Biofilm Disassembly by Interfering with Peptidoglycan Synthesis

d-Amino acids are signals for biofilm disassembly. However, unexpected metabolic pathways severely attenuate the utilization of d-amino acids in biofilm disassembly, resulting in unsatisfactory efficiency. Herein, three-dimensional poly(d-amino acid) nanoparticles (NPs), which possess the ability to block intracellular metabolism, are constructed with the aim of disassembling the biofilms. The obtained poly(α-N-acryloyl-d-phenylalanine)-block-poly(β-N-acryloyl-d-aminoalanine NPs (denoted as FA NPs) present α-amino groups and α-carboxyl groups of d-aminoalanine on their surface, which guarantees that FA NPs can effectively insert into bacterial peptidoglycan (PG) via the mediation of PG binding protein 4 (PBP4). Subsequently, the FA NPs trigger the detachment of amyloid-like fibers that connect to the PG and reduce the number of polysaccharides and proteins in extracellular polymeric substances (EPS). Finally, FA NPs damage the structural stability of EPS and lead to the disassembly of the biofilm. Based on this feature, FA NPs significantly enhance the killing efficacy of encapsulated sitafloxacin sesquihydrate (Sita) by facilitating the penetration of Sita within the biofilm, achieving complete elimination of Staphylococcal biofilm in mice. Therefore, this study strongly demonstrates that FA NPs can effectively improve biofilm disassembly efficacy and provide great potential for bacterial biofilm infection treatment

Additional file 1 of Long-term outcomes of left atrial appendage closure with or without concomitant pulmonary vein isolation:a propensity score matching analysis based on CLACBAC study

Additional file 1: Supplemental table 1. Baseline characteristics of PVI alone groups versus combined group