Additional file 8: of Variable-angle epifluorescence microscopy characterizes protein dynamics in the vicinity of plasma membrane in plant cells

Time-lapse recording for microtubular organization as revealed by VAEM. (AVI 1072 kb

Additional file 1: of Variable-angle epifluorescence microscopy characterizes protein dynamics in the vicinity of plasma membrane in plant cells

Methodology for determining incident angle and penetration depth. (PDF 569  kb

Additional file 6: of Variable-angle epifluorescence microscopy characterizes protein dynamics in the vicinity of plasma membrane in plant cells

Dual-color VAEM indicates clathrin light chain (CLC)–GFP and mCherry-Flot1a showing characteristic localization to separated punctate structures. (AVI 127 kb

Additional file 7: of Variable-angle epifluorescence microscopy characterizes protein dynamics in the vicinity of plasma membrane in plant cells

Rapid turnover of actin filaments in transgenic fABD2-GFP Arabidopsis seedlings in the proximity of plasma membrane. (AVI 1151 kb

Additional file 3: of Variable-angle epifluorescence microscopy characterizes protein dynamics in the vicinity of plasma membrane in plant cells

Dynamics of Golgi apparatuses in the proximity of plasma membrane. (AVI 23044 kb

Additional file 4: of Variable-angle epifluorescence microscopy characterizes protein dynamics in the vicinity of plasma membrane in plant cells

Dynamics of mitochondria in the proximity of plasma membrane. (AVI 23044 kb

Additional file 2: of Variable-angle epifluorescence microscopy characterizes protein dynamics in the vicinity of plasma membrane in plant cells

Figure S1. Organellar dynamics are resolved in high spatio-temporal manner under VAEM. Figure S2. VAEM is applicable to epidermal cells of different tissues from seedlings. Figure S3. CLC-GFP tagged punctate structures correspond to clathrin-coated vesicles and Golgi apparatuses, depending on the angle of incident light. Figure S4. Actin turnover resolved using VAEM. Figure S5. Microtubular organization as revealed by VAEM. Figure S6. SKU5-GFP localizes to plasma membrane and intracellular structures. Figure S7. Distribution of the fluorescence intensity of the diffraction-limited single pCLC2- myristoyl-mGFPA206K spots. Figure S8. Fluorescence correlation spectroscopy (FCS) to examine the fluorescence fluctuation in response to TyrA23 treatment. Figure S9. Analysis on MSD for different trajectories and categorization into various diffusion regimes. Figure S10. Representative kymographs for BOR1 and Lti6a. (PDF 1148 kb