MOESM1 of A novel β-glucosidase from Saccharophagus degradans 2-40T for the efficient hydrolysis of laminarin from brown macroalgae

Additional file 1: Table S1. Kinetic parametersa of Bgl1B in the hydrolysis of different types of substrates

MOESM3 of A novel β-glucosidase from Saccharophagus degradans 2-40T for the efficient hydrolysis of laminarin from brown macroalgae

Additional file 3: Figure S2. TLC analysis for the hydrolysis of pustulan substrate using Bgl1B. The reaction was performed at 40 °C in 20 mM sodium phosphate buffer (pH 6.0) with 0.2% (w/v) pustulan. Abbreviations: Std, standard; Glc, glucose; −, substrate only; +, substrate incubated with 0.2 U of Bgl1B/mg of pustulan for 12 h

MOESM2 of A novel β-glucosidase from Saccharophagus degradans 2-40T for the efficient hydrolysis of laminarin from brown macroalgae

Additional file 2: Figure S1. The phylogenetic tree of Bgl1B and other characterized bacterial β-glucosidases in glycoside hydrolase family 1 (GH1). Thermotoga neapolitana DSM 4359 in GH3 was used to compare between β-glucosidases from GH1 and GH3. The nucleotide sequences of the enzymes were obtained from UniProt and the CAZy database, and aligned using RDP release 11 tool. The phylogenetic tree was drawn using the MEGA 7 program. The accession numbers are indicated after the microorganism names

MOESM4 of A novel β-glucosidase from Saccharophagus degradans 2-40T for the efficient hydrolysis of laminarin from brown macroalgae

Additional file 4: Table S2. Comparison of optimum conditions and substrate specificities of various characterized β-glucosidases (EC 3.2.1.21) in GH1

Additional file 3: Figure S3. of Efficacy and safety of aflibercept in in vitro and in vivo models of retinoblastoma

In Vivo and In vitro experiment with WERI-Rb-1 cell. (A) MICRON image of orthotopic xenotransplantation model with WERI-Rb-1 cells. Five weeks after WERI-Rb-1 cell intravitreal injection, vitreous haziness became worse. (B) Representative H&E staining of the orthotopic xenotransplantation model with WERI-Rb-1 cell. Though WERI-Rb-1 cell was proliferated in the vitreous cavity, there was no subretinal invasion in the orthotopic xenotransplantation model with WERI-Rb-1 cell (saline = 9, aflibercept = 13). (TIF 2898 kb

Additional file 1: Figure S1. of Efficacy and safety of aflibercept in in vitro and in vivo models of retinoblastoma

Automated quantification of angiogenesis in the xenotransplantation model. Upper row showed Lectin staining (green) of microvessels in tumor sections. Lower row represented binarized image (black and white) of Lectin staining. Images were binarized to black and white with a common threshold level, such that white pixels represent lectin-positive cells. The fraction of white pixels, representing lectin-stained blood vessels, was automatically quantified by histogram analysis using Adobe Photoshop CS4. (TIF 706 kb

Additional file 1: Table S1. of Comparison of pressure-controlled ventilation with volume-controlled ventilation during one-lung ventilation: a systematic review and meta-analysis

Search strategies of each database. (DOCX 23 kb

Additional file 1: of Low recurrence rate of anchored conjunctival rotation flap technique in pterygium surgery

Video S1. Surgical procedure of anchored conjunctival rotation flap surgery. (WMV 61807 kb

Additional file 2: of Dysnatremia, its correction, and mortality in patients undergoing continuous renal replacement therapy: a prospective observational study

Subject number according to the change of serum sodium during 24 or 72 hours. (DOC 38 kb

Additional file 1: Table S1. of Clinical implications of carotid artery intima media thickness assessment on cardiovascular risk stratification in hyperlipidemic Korean adults with diabetes: the ALTO study

Mean cIMT values that were used as cutoff values for determining subclinical atherosclerosis. Table S2. Correlation between 10-year cardiovascular risk and cIMT measures UKPDS Framingham Risk Score. Table S3. cIMT measures between UKPDS coronary heart disease risk groups by gender and age. Table S4. Univariate predictors of subclinical atherosclerosis. Table S5. Multivariate independent predictors of subclinical atherosclerosis. Table S6. Mean cIMT according to age and the status of statin use. Figure S1. Receiver operating characteristic curve analysis of conventional risk scores in predicting subclinical atherosclerosis (p = 0.189). (DOCX 787 kb