LPS诱导的肺纤维细胞PTEN基因表达和Akt磷酸化蛋白表达的研究

Objective: To investigate PTEN gene expression and the Akt phosphorylation of protein expression in the LPS-induced lung fibroblast, to initially reveal the relation between PTEN gene and the Akt phosphorylated proteins to LPS-induced lung fibroblast proliferation mechanism. Methods: BrdU experiments was performed to evaluate the LPS-induced lung fibroblast proliferation,  RT-PCR and Western Blot analysis were used to analyze the PTEN gene expression and Western blot was performed to analyze Akt phosphorylated protein expression. Results: PTEN mRNA level of the experimental group were significantly lower than the control group (P<0.05) with LPS simulation for 24h and 72h , and there were no significant difference between the experimental group and control group the experimental group and control group (P>0.05) . PTEN protein expression levels of the experimental group were significantly lower than the control group (P<0.05) , at 72h, and PTEN mRNA levels had no significant differences between these of the experimental and control group at 6h,12h and 24h(p>0.05). Phosphorylation Akt protein level (relative to total Akt protein) was significantly higer than the control group (P<0.05) at 24h and 72h, and phosphorylation Akt protein levels had no significant differences between these of the experimental and control group at 6h and 12h (P>0.05) .Conclusion: PTEN gene and phosphorylation Akt protein involve in LPS-induced lung fibroblast proliferation signal transduction pathway.目的  探讨LPS诱导肺成纤维细胞PTEN基因表达和Akt磷酸化蛋白表达，初步揭示LPS诱导的肺成纤维细胞增殖机制中PTEN基因和Akt磷酸化蛋白的变化情况。方法  通过BrdU实验评价LPS诱导肺成纤维细胞增殖情况，通过RT-PCR和Western Blot分析LPS诱导LPS诱导肺成纤维细胞增殖后细胞内PTEN mRNA水平和蛋白表达情况，通过Western Blot分析LPS诱导肺成纤维细胞增殖后细胞Akt磷酸化蛋白表达情况。结果  在6h、12h时实验组和对照组PTEN mRNA转录水平差异无显著性（P>0.05），24h和72h时实验组PTEN mRNA转录水平显著低于对照组（P<0.05）。在6h、12h和24h时实验组和对照组PTEN mRNA转录水平差异无显著性（P>0.05），在72h时实验组PTEN蛋白表达水平显著低于对照组（P<0.05）。在6h、12h时实验组和对照组Akt磷酸化蛋白（相对总Akt蛋白）差异无显著性（P>0.05），在24h和72h实验组Akt磷酸化蛋白（相对总Akt蛋白）显著低于对照组（P<0.05）。结论  PTEN基因和Akt磷酸化蛋白参与了LPS诱导的肺成纤维细胞增殖信号转导途径

Which digit is larger? Brain responses to number and size interactions in a numerical Stroop task

When comparing the digits of different physical sizes, the processing of numerical value interacts with the processing of physical size. Given the universal use of Arabic numbers in mathematics and daily life, this study aims to elucidate the cognitive processes involved in the interactions of task-relevant and task-irrelevant features during information processing. We investigated this question by examining event-related potential (ERP) using a modified version of the size congruity comparison, which is a Stroop-like task. Numerical value and physical size were varied independently under task-relevant and task-irrelevant conditions. To better examine how the task-irrelevant features modulated the processing of the task-relevant attributes, a neutral condition was included in both tasks. For the physical task, congruent trials showed a less negative N200 response than neutral trials (indicating a facilitation effect), and incongruent trials elicited a larger N450 and smaller late positive complex (LPC) response than neutral trials (indicating an interference effect). For the numerical task, congruent trials showed a larger LPC response than neutral trials (indicating a facilitation effect). These ERP findings indicate that the sources of the facilitation and interference effects appear in different cognitive processes for each task. We further suggest that language characteristics may be a factor in the superior numerical processing exhibited in this study

Optimization of supercritical carbon dioxide extraction of flaxseed oil using response surface methodology

The optimal conditions for the supercritical carbon dioxide (SC-CO2) extraction of flaxseed oil from flaxseed were determined using response surface methodology (RSM). A second-order regression for rotation-orthogonal composite design was used to study the effects of three independent variables: extraction pressure (MPa), extraction temperature (oC) and CO2 flow rate (L/h) on the yield of flaxseed oil. The independent variables were coded at five levels and their actual values selected on the basis of preliminary experiments. The results indicated that the yield of flaxseed oil was beyond 29% at a probability of 95% in the range of extraction pressure: 38.6-42.3 MPa, extraction temperature: 52.3-57.0 oC, and CO2 flow rate: 27.8-31.2 L/h. The optimal extraction conditions were extraction pressure of 41 MPa, extraction temperature of 56 oC and CO2 flow rate of 31 L/h according to the analysis of response surface. In this condition, the experimental yield of flaxseed oil was 29.96%, which was close to the predicted value of 30.52%

Toxicities comparison of rotenone and acetone extract of Tephrosiavogelii and Derris trifoliate against Solenopsis invicta

The high rotenone content and the rotenone crude extract of Tephrosia vogelii and Derris trifoliata were evaluated for its efficacy in the control of red imported fire (RIFA), Solenopsis invicta under both laboratory and field conditions. The acetone extracts of D. trifoliata roots and T. vogelii leaves exhibited strong toxicity to macroergate and micrergate of RIFA. When active ingredients of the crude extracts were convert to rotenone, the activity of the acetone extracts were higher than that of rotenone technical material. At the same time, the extracts showed significant inhibitory effect on walking ability and grasping ability of worker ants and stronger than the effect of 98.6% rotenone technical material. Under field conditions, the 0.01% rotenone-bait, formulated with the acetone extract of D. trifoliata roots and T. vogelii leaves, had the same control effect on RIFA as that of 0.01% fipronil-bait when treated after 30 d. The bait formulated with the extract of D. trifoliata exhibited quicker and higher effect on RIFA than that of rotenone technical material. It was showed that the acetone extracts of D. trifoliata roots and T. vogelii leaves are able to control S. invicta under both laboratory and field conditions and can be used as an effective agent against RIFA

Optical heterodyne micro-vibration detection based on all-fiber acousto-optic superlattice modulation

We propose a configuration of optical heterodyne micro-vibration detection based on an all-fiber acousto-optic superlattic modulation structure that acts as both frequency shifter and reflector, simultaneously. The vibration information within the frequency range between 1 Hz to 150 kHz of a piezoelectric mirror (PZM) has been experimentally measured by using this all-fiber optical heterodyne detection configuration. The minimal measurable vibration amplitude and the resolution are around 0.013 nm and 10 pm in the region of tens to hundreds of kilohertz, respectively. The configuration not only has advantages of compact size, easy alignment and non-contact measurement, but also gains high accuracy, which provides a promising alternative and could be applied in the compact and portable instruments based on optical heterodyne detection